Thermodynamic studies of ligand binding to the human homopentameric glycine receptor using isothermal titration calorimetry

References

• BetzH. 1991. Glycine receptors: Heterogeneous and widespread in the mammalian brain. Trends Neurosci14:458–461.
   PubMed Web of Science ®Google Scholar
• BetzH, LaubeB. 2006. Glycine receptors: Recent insights into their structural organization and functional diversity. J Neurochem97:1600–1610.
   PubMed Web of Science ®Google Scholar
• BizouarnT, van BoxelGI, BhaktaT, JacksonJB. 2005. Nucleotide binding affinities of the intact proton-translocating transhydrogenase from Escherichia coli. Biochim Biophys Acta1708:404–410.
   PubMed Web of Science ®Google Scholar
• BundschuhFA, HannappelA, AnderkaO, LudwigB. 2009. Surf1, associated with leigh syndrome in humans, is a heme-binding protein in bacterial oxidase biogenesis. J Biol Chem284:25735–25741.
   PubMed Web of Science ®Google Scholar
• CascioM, ShenkelS, GrodzickiRL, SigworthFJ, FoxRO. 2001. Functional reconstitution and characterization of recombinant human alpha 1-glycine receptors. J Biol Chem276:20981–20988.
   PubMed Web of Science ®Google Scholar
• ChairesJB. 2008. Calorimetry and thermodynamics in drug design. Annu Rev Biophys37:135–151.
   PubMed Web of Science ®Google Scholar
• ChaoY, FuD. 2004. Thermodynamic studies of the mechanism of metal binding to the Escherichia coli zinc transporter YiiP. J Biol Chem279:17173–17180.
   PubMed Web of Science ®Google Scholar
• CreggJM, TolstorukovI, KusariA, SungaJ, MaddenK, ChappellT. 2009. Chapter 13, expression in the yeast Pichia pastoris. Academic Press; San Diego, California, United States.
   Google Scholar
• DalyR, HearnMT. 2005. Expression of heterologous proteins in Pichia pastoris: A useful experimental tool in protein engineering and production. J Mol Recognit18:119–138.
   PubMed Web of Science ®Google Scholar
• Destoumieux-GarzonD, DuquesneS, PeduzziJ, GoulardC, DesmadrilM, LetellierL, 2005. The iron-siderophore transporter FhuA is the receptor for the antimicrobial peptide microcin J25: Role of the microcin Val11-Pro16 beta-hairpin region in the recognition mechanism. Biochem J389:869–876.
   PubMed Web of Science ®Google Scholar
• FangY, Kolmakova-PartenskyL, MillerC. 2007. A bacterial arginine-agmatine exchange transporter involved in extreme acid resistance. J Biol Chem282:176–182.
   PubMed Web of Science ®Google Scholar
• FreireE. 2008. Do enthalpy and entropy distinguish first in class from best in class?Drug Discov Today13:869–874.
   PubMed Web of Science ®Google Scholar
• Garcia-CalvoM, Ruiz-GomezA, VazquezJ, MoratoE, ValdiviesoF, MayorF. 1989. Functional reconstitution of the glycine receptor. Biochemistry28:6405–6409.
   PubMed Web of Science ®Google Scholar
• GentetLJ, ClementsJD. 2002. Binding site stoichiometry and the effects of phosphorylation on human alpha1 homomeric glycine receptors. J Physiol544:97–106.
   PubMed Web of Science ®Google Scholar
• GrenninglohG, SchmiedenV, SchofieldPR, SeeburgPH, SiddiqueT, MohandasTK, 1990. Alpha subunit variants of the human glycine receptor: Primary structures, functional expression and chromosomal localization of the corresponding genes. EMBO J9:771–776.
   PubMed Web of Science ®Google Scholar
• GriffonN, ButtnerC, NickeA, KuhseJ, SchmalzingG, BetzH. 1999. Molecular determinants of glycine receptor subunit assembly. EMBO J18:4711–4721.
   PubMed Web of Science ®Google Scholar
• GrudzinskaJ, SchemmR, HaegerS, NickeA, SchmalzingG, BetzH, 2005. The beta subunit determines the ligand binding properties of synaptic glycine receptors. Neuron45:727–739.
   PubMed Web of Science ®Google Scholar
• HarveyRJ, DepnerUB, WassleH, AhmadiS, HeindlC, ReinoldH, 2004. GlyR alpha3: An essential target for spinal PGE2-mediated inflammatory pain sensitization. Science304:884–887.
   PubMed Web of Science ®Google Scholar
• HenryCM. 2001. Structure-based drug design. Chem Eng News79:69–74.
   Web of Science ®Google Scholar
• HibbsRE, GouauxE. 2011. Principles of activation and permeation in an anion-selective cys-loop receptor. Nature474:54–60.
   PubMed Web of Science ®Google Scholar
• HilfRJ, DutzlerR. 2008. X-ray structure of a prokaryotic pentameric ligand-gated ion channel. Nature452:375–379.
   PubMed Web of Science ®Google Scholar
• HousdenNG, LoftusSR, MooreGR, JamesR, KleanthousC. 2005. Cell entry mechanism of enzymatic bacterial colicins: Porin recruitment and the thermodynamics of receptor binding. Proc Natl Acad Sci USA102:13849–13854.
   PubMed Web of Science ®Google Scholar
• HwangTL, ShakaAJ. 1995. Water suppression that works. excitation sculpting using arbitrary wave-forms and pulsed-field gradients. J Magnetic Reson Ser A112:275–279.
   Web of Science ®Google Scholar
• InoueK, UenoS, YamadaJ, FukudaA. 2005. Characterization of newly cloned variant of rat glycine receptor alpha1 subunit. Biochem Biophys Res Commun327:300–305.
   PubMed Web of Science ®Google Scholar
• KrellT, Renauld-MongenieG, NicolaiMC, FraysseS, ChevalierM, BerardY, 2003. Insight into the structure and function of the transferrin receptor from Neisseria meningitidis using microcalorimetric techniques. J Biol Chem278:14712–14722.
   PubMed Web of Science ®Google Scholar
• KumamotoE, MurataY. 1996. Glycine current in rat septal cholinergic neuron in culture: Monophasic positive modulation by Zn2+. J Neurophysiol76:227–241.
   PubMed Web of Science ®Google Scholar
• LadburyJE, KlebeG, FreireE. 2010. Adding calorimetric data to decision making in lead discovery: A hot tip. Nat Rev Drug Discov9:23–27.
   PubMed Web of Science ®Google Scholar
• LegendreP. 2001. The glycinergic inhibitory synapse. Cell Mol Life Sci58:760–793.
   PubMed Web of Science ®Google Scholar
• LynchJW. 2009. Native glycine receptor subtypes and their physiological roles. Neuropharmacology56:303–309.
   PubMed Web of Science ®Google Scholar
• LynchJW. 2004. Molecular structure and function of the glycine receptor chloride channel. Physiol Rev84:1051–1095.
   PubMed Web of Science ®Google Scholar
• MarvizonJC, Garcia CalvoM, VazquezJ, MayorFJr, Ruiz GomezA, ValdiviescoF, 1986. Activation and inhibition of 3H-strychnine binding to the glycine receptor by eccles' anions: Modulatory effects of cations. Mol Pharmacol30:598–602.
   PubMed Web of Science ®Google Scholar
• PfeifferF, BetzH. 1981. Solubilization of the glycine receptor from rat spinal cord. Brain Res226:273–279.
   PubMed Web of Science ®Google Scholar
• PfeifferF, GrahamD, BetzH. 1982. Purification by affinity chromatography of the glycine receptor of rat spinal cord. J Biol Chem257:9389-9393.
   PubMed Web of Science ®Google Scholar
• RiquelmeG, MoratoE, LopezE, Ruiz-GomezA, FerragutJA, Gonzalez RosJM, 1990. Agonist binding to purified glycine receptor reconstituted into giant liposomes elicits two types of chloride currents. FEBS Lett276:54–58.
   PubMed Web of Science ®Google Scholar
• RosenbaumDM, CherezovV, HansonMA, RasmussenSG, ThianFS, KobilkaTS, 2007. GPCR engineering yields high-resolution structural insights into beta2-adrenergic receptor function. Science318:1266–1273.
   PubMed Web of Science ®Google Scholar
• SchaggerH, von JagowG. 1991. Blue native electrophoresis for isolation of membrane protein complexes in enzymatically active form. Anal Biochem199:223–231.
   PubMed Web of Science ®Google Scholar
• SikoraCW, TurnerRJ. 2005a. Investigation of ligand binding to the multidrug resistance protein EmrE by isothermal titration calorimetry. Biophys J88:475–482.
   PubMed Web of Science ®Google Scholar
• SikoraCW, TurnerRJ. 2005b. SMR proteins SugE and EmrE bind ligand with similar affinity and stoichiometry. Biochem Biophys Res Commun335:105–111.
   PubMed Web of Science ®Google Scholar
• SineSM, EngelAG. 2006. Recent advances in cys-loop receptor structure and function. Nature440:448–455.
   PubMed Web of Science ®Google Scholar
• UnwinN. 2003. Structure and action of the nicotinic acetylcholine receptor explored by electron microscopy. FEBS Lett555:91–95.
   PubMed Web of Science ®Google Scholar
• VogelN, KluckCJ, MelzerN, SchwarzingerS, BreitingerU, SeeberS, 2009. Mapping of disulfide bonds within the amino-terminal extracellular domain of the inhibitory glycine receptor. J Biol Chem284:36128–36136.
   PubMed Web of Science ®Google Scholar
• WarneT, Serrano-VegaMJ, BakerJG, MoukhametzianovR, EdwardsPC, HendersonR, 2008. Structure of a beta1-adrenergic G-protein-coupled receptor. Nature454:486–491.
   PubMed Web of Science ®Google Scholar
• WeberPC, SalemmeFR. 2003. Applications of calorimetric methods to drug discovery and the study of protein interactions. Curr Opin Struct Biol13:115–121.
   PubMed Web of Science ®Google Scholar
• WeiY, FuD. 2005. Selective metal binding to a membrane-embedded aspartate in the Escherichia coli metal transporter YiiP (FieF). J Biol Chem280:33716–33724.
   PubMed Web of Science ®Google Scholar
• WittigI, BeckhausT, WumaierZ, KarasM, SchaggerH. 2010. Mass estimation of native proteins by blue native electrophoresis: Principles and practical hints. Mol Cell Proteomics9:2149–2161.
   PubMed Web of Science ®Google Scholar
• ZhangW, Hywood PotterKJ, PlantzBA, SchlegelVL, SmithLA, MeagherMM. 2003. Pichia pastoris fermentation with mixed-feeds of glycerol and methanol: growth kinetics and production improvement. J Ind Microbiol Biotechnol30:210–215.
   PubMed Web of Science ®Google Scholar
• ZimmermannK, HeckM, FrankJ, KernJ, VassI, ZouniA. 2006. Herbicide binding and thermal stability of photosystem II isolated from Thermosynechococcus elongatus. Biochim Biophys Acta1757:106–114.
   PubMed Web of Science ®Google Scholar
• ZimmermannP. 2006. The prevalence and significance of PDZ domain-phosphoinositide interactions. 7Biochim Biophys Acta1761:947–956.
   PubMed Web of Science ®Google Scholar