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Original Article

Isolation and Sequencing of Rhizobium Leguminosarum Bv. Trifolii PssN, PssO and PssP Genes Encoding the Proteins Involved in Polymerization and Translocation of Exopolysaccharide

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Pages 1-12 | Received 03 Aug 2000, Published online: 11 Jul 2009

References

  • Altschul S. F., Madden T. L., Schaffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Research 1997; 25: 3389–3402
  • Bairoch A., Bucher P., Hofmann K. The PROSITE database, its status in 1997. Nucleic Acids Research 1997; 25: 217–221
  • Becker A., Niehaus K., Pühler A. Low molecular weight succinoglycan is predominantly produced by Rhizobium meliloti strains carrying a mutated ExoP protein characterized by a periplasmic N-terminal and a missing C-terminal domain. Molecular Microbiology 1995; 16: 191–203
  • Becker A., Pühler A. Specific amino acid substitutions in the proline-rich motif of the Rhizobium meliloti ExoP protein result in enhanced production of low-molecular-weight succinoglycan at the expense of high-molecular-weight succinoglycan. Journal of Bacteriology 1998a; 180: 395–399
  • Becker A., Pühler A. Production of exopolysac-charides. Rhizobiaceae, H. P. Spaink, A. Kondorosi, P. J.J Hooykaas. Kluwer Academic Publishers, Dordrecht, Boston, London 1998b; 97–118
  • Borthakur D., Barker R. F., Latchford J. W., Rossen L., Johnston A. W.B. Analysis of pss genes in Rhizobium leguminosarum required for exopolysaccharide synthesis and nodulation of peas: their primary structure and their interaction with psi and other nodulation genes. Molecular General Genetics 1988; 213: 155–162
  • Borthakur D., Johnston A. W.B. Sequence of psi, a gene on the symbiotic plasmid of Rhizobium phaseoli which inhibits exopolysaccharide synthesis and nodulation and demonstration that its transcription is inhibited by psr, another gene on the symbiotic plasmid. Molecular General Genetics 1987; 207: 149–154
  • Canter Cremers H. C.J., Batley M., Redmond J. W., Wijfjes A. H.M., Lugtenberg B. J.J, Wijffelman C. A. Distribution of O-acetyl groups in the exopolysaccharide synthesized by Rhizobium leguminosarum strain is not determined by the Sym plasmid. Journal of Biological Chemistry 1991; 266: 9556–9564
  • Chung J., Zhang Y., Adler B. The capsule biosyn-thetic locus of Pasteurella multocida A:l. FEMS Microbiology Letters 1998; 15: 289–296
  • Doublet P., Vincent C, Grangeasse C, Cozzone A. J., Duclos B. On the binding of ATP to the autophos-phorylating protein, Ptk, of the bacterium Acinetobacter johnosonii. FEBS Letters 1999; 445: 137–143
  • Frosch M., Müller D., Bousset K., Müller A. Conserved outer membrane protein of Neiseria meningitidis involved in capsule expression. Infection and Immunity 1992; 60: 798–803
  • Gamier J., Gibrat J.-F., Robson B. GOR secondary structure prediction method version IV. Methods in Enzymology, R. F. Doolittle, 1996; vol. 266: 540–553
  • Gonzales J. E., Semino C, Wang L.-X., Castellano-Torres L. E., Walker G. C. Biosynthetic control of molecular weight in the polymerization of the octasaccharide subunits of succinoglycan, a symbiotically important exopolysaccharide of Rhizobium meliloti. Proceedings of National Academy of Science of the United States of America 1998; 95: 13477–13482
  • Hofmann K., Stoffel W. TMbase - A database of membrane spanning proteins segments. Biological Chemistry Hoppe-Seyler 1993; 347: 166
  • Hollingsworth R. I., Dazzo F. B., Hallenga K., Musselman B. The complete structure of the trifoliin A lec-tin-binding capsular polysaccharide of Rhizobium trifolii 843. Carbohydrate Research 1988; 172: 97–112
  • Huang J., Schell M. Molecular characterization of the eps gene cluster of Pseudomonas solanacearum and its transcriptional regulation at a single promoter. Molecular Microbiology 1995; 16: 977–989
  • Ilan O., Bloch Y., Frankel G., Ullrich H., Geider K., Rosen-Shine I. Protein tyrosine kinases in bacterial pathogens are associated with virulence and production of exopolysaccharide. EMBO Journal 1999; 18: 3241–3248
  • Ivashina T. V., Khmelnitsky M. I., Shlyapnikov M. G., Kanapin A. A., Ksenzenko V. N. The pss4 gene from Rhizobium leguminosarum bv viciae VF39: Cloning, sequence and the posssible role in polysaccharide production and nodule formation. Gene 1994; 150: 111–116
  • Janczarek M., Król J., Skorupska A. The pssB gene product of Rhizobium leguminosarum bv trifolii is homologous to a family of inositol monophosphatases. FEMS Microbiology Letters 1999; 173: 319–325
  • Kovach M. E., Elzer P. H., Hill D. S., Robertson G. T., Farris M. A., Roop R. M., II, Peterson K. M. Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes. Gene 1995; 166: 175–176
  • Kroll J. S., Loynds B., Brophy L. N., Moxon R. The bex locus in encapsulate Haemophilus influenzae: a chromosomal region involved in capsule polysaccharide export. Molecular Microbiology 1990; 4: 1853–1862
  • Król J., Skorupska A. Identification of genes in Rhizobium leguminosarum bv trifolii whose products are homologous to a family of ATP-binding proteins. Microbiology 1997; 143: 1389–1394
  • Król J. E., Wielbo J., Mazur A., Kopciñska J., õocka B., Golinowski W., Skorupska A. Molecular charcterization of pssCDE genes of Rhizobium leguminosarum bv trifolii strain TA1: pssD mutant is affected in exopolysaccharide synthesis and endocytosis of bacteria. Molecular Plant-Microbe Interactions 1998; 11: 1142–1148
  • Kyte J., Doolittle R. F. A simple method for displaying the hydropathic character of a protein. Journal of Molecular Biology 1982; 157: 105–132
  • Leigh J. A., Walker G. C. Exopolysaccharides of Rhizobium: synthesis, regulation and symbiotic function. Trends in Genetics 1994; 10: 63–67
  • Lupas A., Van Dyke M., Stock J. Predicting coiled coils from protein sequences. Science 1991; 252: 1162–1164
  • Morona R., Van Den Bosch L., Daniels C. Evaluation of Wzz/MPA1/MPA2 proteins based on the presence of coiled-coils region. Microbiology 2000; 146: 1–4
  • Nielsen H., Engelbrecht J., Brunak S., von Heijne G. Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Protein Engineering 1997; 10: 1–6
  • Paulsen I. T., Beness A. M., Saier M. H., Jr. Computer-based analyses of the protein constituents of transport systems catalysing export of complex carbohydrates in bacteria. Microbiology 1997; 143: 2685–2699
  • Pearson W. R., Lipman D. J. Improved Tools for Biological Sequence Analysis. Proceedings of National Academy of Science of the United States of America 1988; 85: 2444–2448
  • Sadykov M. R., Ivashina T. V., Kanapin A. A., Shlyapnikov M. G., Ksenzenko V. N. Structural and functional organization of the exopolysaccharide biosynthesis genes in Rhizobium leguminosarum bv viciae VF39. Molecular Biology (Russ.) 1998; 5: 665–671
  • Sambrook J., Fritsch E. F., Maniatis T. Molecular Cloning: a Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 1989
  • Stingele F., Neeser J.-R., Mollet B. Identification of the eps (exopolysaccharide) gene cluster from Streptococcus thermophilus Sfi6. Journal of Bacteriology 1996; 178: 1680–1690
  • van Workum W. A.T., Canter Cremers H. C.J., Wijfies A. H.M., van der Kolk C, Wijffelman C. A., Kijne J. W. Cloning and characterization of four genes of Rhizobium leguminosarum bv trifolii involved in exopolysaccharide production and nodulation. Molecular Plant-Microbe Interactions 1997; 10: 290–301
  • Vincent C, Doublet P., Grangeasse C, Vaganay E., Cozzone A., Duclos B. Cells of Escherichia coli contain a protein-tyrosine kinase, Wzc, and a phosphotyro-sine-protein phosphatase, Wzb. Journal of Bacteriology 1999; 181: 3472–3477
  • Vojnov A. A., Zorreguieta A., Dow M., Daniels M. J., Dankert M. A. Evidence for a role for the gumB and gumC gene products in the formation of xanthan from its pentasaccharide repeating unit by Xanthomonas campestris. Microbiology 1998; 144: 1487–1493
  • Walker J. E., Saraste M., Runswick M. J., Gay N. J. Distantly related sequences in the α- and β-subunits of ATP synthase myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold. EMBO Journal 1982; 1: 945–951
  • Ward C. K., Inzana T. J. Identification and characterization of a DNA region involved in the export of capsular polysaccharide by Actinobacillus pleuropneumoniae serotype 5a. Infection and Immunity 1997; 65: 2491–2496
  • Whitfield C, Amor P. A., Köplin R. Modulation of the surface architecture of Gram-negative bacteria by the action of surface polymer: lipidA-core ligase and by determinants of polymer chain length. Molecular Microbiology 1997; 23: 629–638

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