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Original Article

Light Microscopic Detection of BCR-ABL Transcripts after In-Cell RT-PCR: Fusion Gene Expression Might Correlate with Clinical Evolution of Chronic Myeloid Leukemia

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Pages 383-396 | Accepted 10 May 1999, Published online: 01 Jul 2009

References

  • Nowell P. C., Hungerfold D. A. A minute chromosome in human granulocytic leukemia. Science 1960; 32: 1497–1501
  • Rowley J. D. A novel consistent chromosome abnormality in chronic myelogenous leukemia detected by quinacrine fluorescence and Giemsa staining. Nature 1973; 243: 290–293
  • Melo J. The diversity of BCR-ABL fusion proteins and their relationship to leukemia phenotype. Blood 1996; 88: 2375–2384
  • de Klein A., van Kessel A. G., Grosveld G., Bartram C. R., Hagemeijer A., Bootsma D., Spurr N. K., Heisterkamp N., Groffen J., Stepherson J. R. A cellular oncogene is translocated to the Philadelphia chromosome in chronic myelocytic leukemia. Nature 1982; 300: 765–767
  • Heisterkamp N., Stephenson J. R., Groffen J., Hansen P. R, de Klein A., Bertran C. R. Localisation of the c-abl oncogene adjacent to a translocation breakpoint in chronic myelocytic leukemia. Nature 1983; 306: 239–242
  • Shtivelman E., Lifshitz B., Gale R. P., Roe B. A., Canaani E. Fused transcript of abl and bcr genes in chronic myeloid leukemia. Nature 1985; 315: 550–554
  • Chan L. L., Karhi K. K., Rayter S. I., Heisterkamp N., Eridani S., Powles R., Lawler S. D., Groffen J., Foulkes J. G., Greaves M. F., Wiedemann L. M. A novel ABL protein expressed in Philadelphia positive acute lymphoblastic leukemia. Nature 1987; 325: 631–635
  • Hochhaus A., Reiter A., Sklapny H., Melo J. V., Sick C., Berger U., Guo J. Q., Arlinghaus R. B., Hehlmann R., Goldman J. M., Cross N. C.P. A novel BCR-ABL fusion gene (e6a2) in a patient with Philapelphia chromosome negative chronic myelogeneous leukemia. Blood 1996; 88: 2236–2240
  • Okamoto K., Karasawa M., Sakai H., Ogura H., Morita K., Naruse K. A novel acute lymphoid leukemia type BCR/ABL transcript in chronic myelogenous leukemia. British Journal of Haematology 1997; 96: 611–613
  • Saglio G., Guerrasio A., Rosso C., Zaccaria A., Tassinari A., Serra A., Rege-Cambrin G., Mazza U., Cavosto F. New type of BCR/ABL junction in Philadelphia chromosome positive leukemia. Blood 1990; 76: 1819–1824
  • Guerrasio A. Molecular insights in chronic myeloid leukemia and in other Ph positive human leukemias. Reviews in Clinical and Experimental Hematology 1997; 2: 52–75
  • Sklar J. L., Costa J. G. Principles of cancer management: Molecular pathology. Principles and practice of oncology, V. De Vita, S. Hellman, S. Rosenberg. Lippincott-Raven Publishers, Philadelphia 1997; 59–84
  • Radich J., Gehly G., Cooley T., Bryant E., Clift R. A., Collins S., Edmans S., Kirk J., Lee A., Kessler P., Schoch G., Buchner C. D., Sullivan K. M., Appelbaum F. R., Thomas E. D. PCR detection of the BCR-ABL fusion transcript after allogeneic marrow transplantation for chronic myeloid leukemia: results and implications in 346 patients. Blood 1995; 13: 1704–1713
  • Nolte M., Werner M., Ewig M., et al. Megakaryocytes carry the fused BCR-ABL gene in chronic myeloid leukaemia: a fluorescence in situ hybridization analysis from bone marrow biopsies. Virchows Archives 1996; 427: 561–565
  • Sinclair P. B., Green A. R., Grace C. D., Proffitt J., Wilber K., Nacheva E. P. (1997) Triple probe three colour FISH system for BCR-ABL detection – further improvements. Proceedings of the 39th Annual Meeting of the American Society of Hematology, San Diego, 1997, Abstract 355
  • Gabert J., Lafage M., Maraninchi D., Thuret I., Carcassonne Y., Manoni P. Detection of residual bcr/abl translocation by polymerase chain reaction in myeloid leukemia patients after bone-marrow transplantation. Lancet 1989; 11: 1125–1128
  • Melo J. V., Hochhaus A., Yan X. H., Goldman J. M. Lack of correlation between ABL-BCR expression and response to interferon-alpha in chronic myeloid leukaemia. British Journal of Haematology 1996; 92: 684–686
  • Costello R., Kirk J., Gabert J. Value of PCR analysis for long term survivors after allogeneic bone marrow transplant for chronic myelogenous leukemia: A comparative study. Leukemia and Lymphoma 1996; 20: 239–243
  • van Rhee F., Lin F., Cross N. C., Reid C. D., Lakhani A. K., Szydlo R. M., Goldman J. M. Detection of residual leukaemia more than 10 years after allogeneic bone marrow transplantation for chronic myelogenous leukaemia. Bone Marrow Transplantation 1994; 14: 609–612
  • Lion T. Clinical implications of qualitative and quantitative polymerase chain reaction analysis in the monitoring of patients with chronic myelogenous leukemia. The European Investigators on Chronic Myeloid Leukemia Group. Bone Marrow Transplantation 1994; 14: 505–509
  • Testoni N., Martinelli G., Farabegoli P., Zaccaria A., Amabile M., Raspadori D., Pelliconi S., Zuffa E., Carboni C., Tura S. A new method of “in-cell reverse transcriptase-polymerase chain reaction” for the detection of BCR/ABL transcript in chronic myeloid leukemia patients. Blood 1996; 87: 3822–3827
  • Pachmann K., Zhao S., Kantarjian H., Andreeff M., et al (1997) In situ amplification of BCR-ABL sequences for analysis of mRNA expression at the single cell level in chronic myeloid leukemia. Proceedings of the 39th Annual Meeting of the American Society of Hematology, San Diego, 1997, Abstract 4008
  • Preudhomme C., Chams Eddine L., Roumier C., Soenen V., Lai J. L., Cosson A., Fenaux P. (1997) In situ RT-PCR for the detection of BCR-ABL transcript in chronic myeloid leukemia: comparison with conventional cytogenetic, FISH and RT-PCR results. Proceedings of the 39th Annual Meeting of the American Society of Hematology, San Diego, 1997, Abstract 4012
  • Chang C., Glassman A. B., Bueso-Ramos C. E. Applicability of direct in situ reverse-transcription-polymerase chain reaction on bone marrow smears. Ann Clin Lab Sci 1998; 28: 34–42
  • Bales C. E., Durfee G. R. Cytologic Techniques. Diagnostic Cytology and its Histopathologic Bases, 4th ed., L. G. Koss. J. B. Lippincott, Philadelphia 1992; 1452–1515
  • Patel V. G., Shum-Siu A., Heniford B., Wieman T., Hendler F. Detection of epidermal growth factor receptor mRNA in tissue sections from biopsy specimens using in-situ polymerase chain reaction. Am J Pathol 1994; 144: 7–14
  • Costello R., Lafage M., Toiron Y., Brunei V., Sainty D., Arnoulet C., Mozziconacci M. J., Bouabdallah R., Gastaut J. A., Maraninchi D. Philadelphia chromosome-negative chronic myeloid leukaemia: a report of 14 new cases. British Journal of Haematology 1995; 90: 346–352
  • San Miguel J., van Dongen J., Bartram C., Parreira A., Wormann B., . Investigation of minimal residual disease (MRD) in acute leukemia (AL): International standardization and evaluation. Cancer Research Supported under BIOMED 1, S. Baig, et al. IOS Press, Amsterdam 1998; 300–306
  • Zou W., Durand-Gasselin I., Dulioust A., Maillot M-C, Galanaud P., Emilie D. Quantification of cytokine gene expression by competitive PCR using colorimetric assay. European Cytokine Network 1995; 6: 257–264
  • Kawasaki E. S., Clark S. S., Coyne M. Y., Smith S. D., Champlin R., Witte O. N., McCormic F. P. Diagnosis of chronic myeloid and acute lymphocytic leukemias by detection of leukemia-specific mRNA sequences amplified in vitro. Proceedings of the National Academy of Science of USA 1988; 85: 5698–5702
  • Chomezynski P., Sacchi N. Single step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction. Analytical Biochemistry 1987; 162: 156–159
  • Kwok S., Higushi R. Avoiding false positives with PCR. Nature 1989; 339: 237–238
  • Komminoth P., Long A. In-situ polymerase chain reaction. An overview of methods, applications and limitations of a new molecular technique. Virchows Archives B 1993; 64: 67–73
  • Teo I. A., Shaunak S. Polymerase chain reaction in situ: an appraisal of an emerging technique. Histochemistry Journal 1995; 27: 647–659
  • Crisan D., Chen S-T., Weil S. C. Polymerase chain reaction in the diagnosis of chromosomal breakpoints. Hematology/Oncology Clinics of North America 1994; 8: 725–751
  • Jinno Y., Yoshiura K., Niikawa N. Use of psoralen as extinguisher of contaminated DNA in PCR. Nucleic Acid Research 1990; 18: 6739
  • Wetzler M., Talpaz M., van Etten R. A., Hirsh-Ginsberg C., Beran M., Kurzrock R. Subcellular localization of bcr, abl and BCR-ABL proteins in normal and leukemic cells and correlation of expression with myeloid differentiation. Journal of Clinical Investigation 1993; 92: 1925–1931
  • Wetzler M., Kurzrock R., Lowe D. G., Kantarjian H., Gutterman J. U., Talpaz M. Alteration in bone marrow adherent layer growth factor expression: A novel mechanism of chronic myelogenous leukemia progression. Blood 1991; 78: 2400–2407
  • Gordon M. Y., Goldman M. J. Cellular and molecular mechanisms in chronic myeloid leukemia: Biology and treatment. British Journal of Haematology 1996; 95: 10–20
  • Keating A., Wang X-N, Laraya P. Variable transcription of BCR-ABL by Ph- cells arising from hematopoietic progenitors in chronic myeloid leukemia. Blood 1994; 83: 1744–1749
  • Gotoh A., Broxmeyer H. E. The function of BCR/ABL and related protooncogenes. Current Opinion in Hematology 1997; 4: 3–11
  • Saglio G., Pane F., Martinelli G., Guerrasio A. Bcr/abl transcripts and leukemia phenotype: An unsolved puzzle. Leukemia and Lymphoma 1997; 26: 281–286
  • Collins S., Kubonishi I., Miyoshi I., Groudine M. Altered transcription of the c-abl oncogene in K-562 and other chronic myelogenous leukemia cells. Science 1984; 225: 72–74
  • Kelliher M., Knott A., McLaughlin J., Witte O. N., Rosenberg N. Differences in oncogenic potency but not target cell specifity distinguish two forms of BCR/ABL oncogene. Molecular Cell Biology 1991; 11: 4710–4718
  • Williamson I., Gautier D., Leibowitz D. (1996) BCR/ABL expression is decreased when a downstream silencer is present on the PHI chromosome. Proceedings of the 38th Annual Meeting of the American Society of Hematology, Orlando, 1996, Abstract 2279
  • Dreazen O., Berman M., Gale R. P. Molecular abnormality of BCR and ABL in chronic myelogenous leukemia associated with long chronic phase. Blood 1988; 71: 797–804
  • Biernaux C., Loos M., Sels A., Huez G., Stryckmans P. Detection of major BCR-ABL gene expression at a very low level in blood cells of some healthy individuals. Blood 1995; 86: 3118–3122
  • Lion T., Izraeli S., Henn T., Gaiger A., Morr W., Gadner H. Monitoring of residual disease in chronic myelogenous leukemia by quantitative polymerase chain reaction. Leukemia 1990; 6: 495–501
  • Gaiger A., Henn T., Horth E., Geissler G., Mitterbauer G., Maier-Dobersberger T., Greinix H., Mannhalter C., Haas O. A., Lechner K., Lion T. Increase of BCR/ABL chimeric mRNA expression in tumor cells of patients with chronic myeloid leukemia precedes disease progression. Blood 1995; 86: 2371–2378
  • Kantarjian H. M., Keating M. J., Talpaz M., Walters R. S., Smith T. L., Cork A., McCredie K. B., Freireich E. J. Chronic myelogenous leukemia in blast crisis. Analysis of 242 patients. American Journal of Medicine 1987; 83: 445–454
  • Cabot G. F., Bentz M., Scholl C., Moos M., Fischer K., Lichter P., Dohner H. High incidence of a second BCR/ABL fusion in chronic myeloid leukemia revealed by interphase cytogenetic analysis on blood and bone marrow smears. Cancer Genetics and Cytogenetics 1996; 87: 107–111
  • Guerrasio A., Serra A., Gottardi E., Cilloni D., Vischia F., Parvis G., Cilly V., Saglio G. Molecular events in chronic myeloid leukemia progression. Leukemia 1995; 11: 519–521

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