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Biotechnic & Histochemistry Volume 84, 2009 - Issue 5
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Research Article

Protein fixation and antigen retrieval: chemical studies

TJ O'Leary Office of Research and Development, Veterans Health Administration, Department of Veterans Affairs, Washington, DCCorrespondence[email protected]
,
CB Fowler Office of Research and Development, Veterans Health Administration, Department of Veterans Affairs, Washington, DC; Department of Biophysics, Armed Forces Institute of Pathology, 1413 Research Boulevard, Rockville, MD
,
DL Evers Office of Research and Development, Veterans Health Administration, Department of Veterans Affairs, Washington, DC; Department of Biophysics, Armed Forces Institute of Pathology, 1413 Research Boulevard, Rockville, MD
&
JT Mason Department of Biophysics, Armed Forces Institute of Pathology, 1413 Research Boulevard, Rockville, MD
Pages 217-221 | Published online: 03 Nov 2009

References

  • Fowler CB, O'Leary TJ, Mason JT. Modeling formalin fixation and histological processing with ribonuclease A: effects of ethanol dehydration on reversal of formaldehyde cross-links. Lab. Invest.. 2008; 88:785–791.
  • Fox CH, Johnson FB, Whiting J, Roller PP. Formaldehyde fixation. J. Histochem. Cytochem.. 1985; 33:845–853.
  • Mason JT, O'Leary TJ. Effects of formaldehyde fixation on protein secondary structure: a calorimetric and infrared spectroscopic investigation. J. Histochem. Cytochem.. 1991; 39:225–229.
  • Metz B, Kersten GF, Baart GJ, de Jong A, Meiring H, ten Hove J, van Steenbergen MJ, Hennink WE, Crommelin DJ, Jiskoot W. Identification of formaldehyde-induced modifications in proteins: reactions with insulin. Bioconjug. Chem.. 2006; 17:815–822.
  • Metz B, Kersten GF, Hoogerhout P, Brugghe HF, Timmermans HA, de Jong A, Meiring H, ten Hove J, Hennink WE, Crommelin DJ, Jiskoot W. Identification of formaldehyde-induced modifications in proteins: reactions with model peptides. J. Biol. Chem.. 2004; 279:6235–6243.
  • Rait VK, O'Leary TJ, Mason JT. Modeling formalin fixation and antigen retrieval with bovine pancreatic ribonuclease A. I. Structural and functional alterations. Lab. Invest.. 2004a; 84:292–299.
  • Rait VK, Xu L, O'Leary TJ, Mason JT. Modeling formalin fixation and antigen retrieval with bovine pancreatic RNase A. II. Interrelationship of cross-linking, immunoreactivity, and heat treatment. Lab. Invest.. 2004b; 84:300–306.
  • Shi SR, Cote RJ, Taylor CR. Antigen retrieval techniques: current perspectives. J. Histochem. Cytochem.. 2001; 49:931–937.
  • Sompuram SR, Vani K, Bogen SA. A molecular model of antigen retrieval using a peptide array. Am. J. Clin. Pathol.. 2006a; 125:91–98.
  • Sompuram SR, Vani K, Hafer LJ, Bogen SA. Antibodies immunoreactive with formalin-fixed tissue antigens recognize linear protein epitopes. Am. J. Clin. Pathol.. 2006b; 125:82–90.
  • Sompuram SR, Vani K, Messana E, Bogen SA. A molecular mechanism of formalin fixation and antigen retrieval. Am. J. Clin. Pathol.. 2004; 121:190–199.
  • Srinivasan M, Sedmak D, Jewell S. Effect of fixatives and tissue processing on the content and integrity of nucleic acids. Am. J. Pathol.. 2002; 161:1961–1971.

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