331
Views
10
CrossRef citations to date
0
Altmetric
Research Article

Time-lapse, single cell based confocal imaging analysis of caspase activation and phosphatidylserine flipping during cellular apoptosis

, , , , , , , , & show all
Pages 181-187 | Accepted 23 Nov 2009, Published online: 05 Mar 2010

References

  • Bijur GN, De Sarno P, Jope RS (2000) Glycogen synthase kinase-3beta facilitates staurosporine- and heat shock-induced apoptosis. Protection by lithium. J. Biol. Chem. 275: 7583–7590.
  • Cohen GM (1997) Caspases: the executioners of apoptosis. Biochem. J. 326: 1–16.
  • Devitt A, Pierce S, Oldreive C, Shingler WH, Gregory CD (2003) CD14-dependent clearance of apoptotic cells by human macrophages: the role of phosphatidylserine. Cell Death Differ. 10: 371–382.
  • Dragovich T, Rudin CM, Thompson CB (1998) Signal transduction pathways that regulate cell survival and cell death. Oncogene 17: 3207–3213.
  • Fadok VA, Bratton DL, Rose DM, Pearson A, Ezekewitz RA, Henson PM (2000) A receptor for phosphatidylserine-specific clearance of apoptotic cells. Nature 405: 85–90.
  • Gescher A (2000) Staurosporine analogues-pharmacological toys or useful antitumour agents? Crit. Rev. Oncol. Hematol. 34: 127–135.
  • Gurskaya NG, Fradkov AF, Pounkova NI, Staroverov DB, Bulina ME, Yanushevich YG, Labas YA, Lukyanov S, Lukyanov KA (2003) A colourless green fluorescent protein homologue from the non-fluorescent hydromedusa Aequorea coerulescens and its fluorescent mutants. Biochem. J. 373: 403–408.
  • Hengartner MO (2000) The biochemistry of apoptosis. Nature 407: 770–776.
  • Hoffmann PR, Kench JA, Vondracek A, Kruk E, Daleke DL, Jordan M, Marrack P, Henson PM, Fadok VA (2005) Interaction between phosphatidylserine and the phosphatidylserine receptor inhibits immune responses in vivo. J. Immunol. 174: 1393–1404.
  • Jacobson MD, Burne JF, King MP, Miyashita T, Reed JC, Raff MC (1993) Bcl-2 blocks apoptosis in cells lacking mitochondrial DNA. Nature 361: 365–369.
  • Kerr JF, Wyllie AH, Currie AR (1972) Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics. Br. J. Cancer 26: 239–257.
  • Lopez E, Ferrer I (2000) Staurosporine- and H-7-induced cell death in SH-SY5Y neuroblastoma cells is associated with caspase-2 and caspase-3 activation, but not with activation of the FAS/FAS-L-caspase-8 signaling pathway. Brain Res. Mol. Brain Res. 85: 61–67.
  • Lovborg H, Gullbo J, Larsson R (2005) Screening for apoptosis - classical and emerging techniques. Anticancer Drugs 16: 593–599.
  • Lovborg H, Nygren P, Larsson R (2004) Multiparametric evaluation of apoptosis: effects of standard cytotoxic agents and the cyanoguanidine CHS 828. Mol. Cancer Ther. 3: 521–526.
  • Matsura T, Serinkan BF, Jiang J, Kagan VE (2002) Phosphatidylserine peroxidation/externalization during staurosporine-induced apoptosis in HL-60 cells. FEBS Lett. 524: 25–30.
  • Matz MV, Fradkov AF, Labas YA, Savitsky AP, Zaraisky AG, Markelov ML, Lukyanov SA (1999) Fluorescent proteins from nonbioluminescent Anthozoa species. Nat. Biotechnol. 17: 969–973.
  • Menaker RJ, Ceponis PJ, Jones NL (2004) Helicobacter pylori induces apoptosis of macrophages in association with alterations in the mitochondrial pathway. Infect. Immun. 72: 2889–2898.
  • Nicholson DW, Thornberry NA (1997) Caspases: killer proteases. Trends Biochem. Sci. 22: 299–306.
  • Nicholson DW, Thornberry NA (2003) Apoptosis. Life and death decisions. Science 299: 214–215.
  • Roman V, Billard C, Kern C, Ferry-Dumazet H, Izard JC, Mohammad R, Mossalayi DM, Kolb JP (2002) Analysis of resveratrol-induced apoptosis in human B-cell chronic leukaemia. Br. J. Haematol. 117: 842–851.
  • Ruysschaert JM, elOuahabi A, Willeaume V, Huez G, Fuks R, Vandenbranden M, Di Stefano P (1994) A novel cationic amphiphile for transfection of mammalian cells. Biochem. Biophys. Res. Commun. 203: 1622–1628.
  • Savill J (1997) Recognition and phagocytosis of cells undergoing apoptosis. Br. Med. Bull. 53: 491–508.
  • Shi Y (2002) Mechanisms of caspase activation and inhibition during apoptosis. Mol. Cell 9: 459–470.
  • Studzinski GP. (1999) Analysis of Cell Death by Flow and Laser-Scanning Cytometry. Oxford University Press, New York. 57–80.
  • Tamaoki T, Nomoto H, Takahashi I, Kato Y, Morimoto M, Tomita F (1986) Staurosporine, a potent inhibitor of phospholipid/Ca++dependent protein kinase. Biochem. Biophys. Res. Commun. 135: 397–402.
  • Tang D, Lahti JM, Kidd VJ (2000) Caspase-8 activation and bid cleavage contribute to MCF7 cellular execution in a caspase-3-dependent manner during staurosporine-mediated apoptosis. J. Biol. Chem. 275: 9303–9307.
  • Tesauro M, Thompson WC, Moss J (2006) Effect of staurosporine-induced apoptosis on endothelial nitric oxide synthase in transfected COS-7 cells and primary endothelial cells. Cell Death Differ. 13: 597–606.
  • Valero A, Merino F, Wolbers F, Luttge R, Vermes I, Andersson H, van den Berg A (2005) Apoptotic cell death dynamics of HL60 cells studied using a microfluidic cell trap device. Lab. Chip 5: 49–55.
  • Xue LY, Chiu SM, Oleinick NL (2003) Staurosporine-induced death of MCF-7 human breast cancer cells: a distinction between caspase-3-dependent steps of apoptosis and the critical lethal lesions. Exp. Cell Res. 283: 135–145.
  • Zou J, Ye Y, Welshhans K, Lurtz M, Ellis AL, Louis C, Rehder V, Yang JJ (2005) Expression and optical properties of green fluorescent protein expressed in different cellular environments. J. Biotechnol. 119: 368–378.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.