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Stain Technology Volume 52, 1977 - Issue 5
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Original Article

The Use of Elemental Iodine to Enhance Staining of Thin Sections to Be Viewed in the Electron Microscope

M. G. Williams Department of Anatomy, the University of Texas Health Science Center at San Antonio, San Antonio, Texas, 78284
&
E. K. Adrian Department of Anatomy, the University of Texas Health Science Center at San Antonio, San Antonio, Texas, 78284
Pages 269-272 | Published online: 12 Jul 2009

References

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  • Ernst B., Bostelmann W., Englemann C. Iodine-labeled dextran for electron-optical demonstration of transport processes. Acta Biol. Med. Ger. 1969; 23: 345–350
  • Flood P. R. Preliminary experiments with iodine in electron opaque stains for ultrathin sections. Septieme Congrès International De Microscopie Electronique, Grenoble 1970; 431
  • Kellenberger E., Ryter A., Sechaud J. Electron microscope study of DNA-containing plasms. II. Vegetative and mature phage DNA as compared with normal bacterial nucleoids in different physiological states. J. Biophys. Biochem. Cytol. 1958; 4: 671–478
  • Mekler L. B., Klimenko S. M., Dobrezov G. E., Naumova V. K., Hoffman Y. P., Zkdanov V. M. Cytochemical and immunochemical analysis at the electron microscopy level: obtaining contrasting antibodies by use of iodine. Nature 1964; 203: 717–719
  • Reynolds E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 1963; 17: 208–212
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  • Seaman A. R., Hanker J. S., Seligman A. M. Electron microscopic demonstration of cytochemical functional groups of macromolecules by the use of new electron opaque reagents. J. Histochem. Cytochem. 1961; 9: 596–597
  • Silvester N. R., Burge R. E. A quantitative estimation of the uptake of the two new electron stains by the cytoplasmic membrane of ram sperm. J. Biophys. Biochem. Cytol. 1959; 6: 179–188
  • Spurr A. R. A low-viscosity epoxy resin embedding medium for electron microscopy. J. Ultrastruct. Res. 1969; 26: 31–43
  • Stewart M. Organic stains for electron microscopy. J. Microsc. (Oxf.) 1973; 97: 381–383
  • Trump B. F., Bulger R. E. New ultrastructural characteristics of cells fixed in a glutaraldehyde-osmium tetroxide mixture, Lab. Invest. 1966; 15: 368–79

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