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Stain Technology Volume 54, 1979 - Issue 1
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Original Article

Zinc Chloride Methylene Blue, Ii. Preparation of Giesma and Wright Type Low Azure B Blood Stains from Dichromate Oxidized Commercial Dye

R. D. Lillie Department of Pathology, Louisiana State University Medical Center, New Orleans, Louisana, 70112
&
Patricia T. Donaldson Department of Pathology, Louisiana State University Medical Center, New Orleans, Louisana, 70112
Pages 41-46 | Published online: 12 Jul 2009

References

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  • Chenzinsky C. Zur Lehre von Mikroorganismus des Malariafiebcn. Zbl. Bakt. 1888; 83/15: 457–560
  • Donaldson P. T., Russo A., Reynolds C., Lillie R. D. Borax methylene blue: a spectroscopic and staining study. Stain Technol. 1978; 53: 225–227
  • Giemsa G. Färbungsmethoden für Malariaparasiten. Zbl. Bakt. (Orig.) 1902; 31: 429–430
  • Holmes W. C., French R. W. The oxidation products of methylene blue. Stain Technol. 1926; 1: 17–26
  • Jenner L. A new preparation for fixing and staining blood. Lancet 1899; 1: 370
  • Kolthoff J. M., Sandell E. B. Inorganic Analyses3rd ed. MacMillan, New York 1953
  • Leishman W. B. A simple and rapid method of producing Romanowsky staining in malarial and other blood films. Brit. Med. J. 1901; 2: 357–358
  • Lillie R. D. Studies on polychrome methylene blue. II. Acid oxidation methods of polychroming. Stain Technol. 1942; 17: 97–110
  • Lillie R. D. Studies on polychrome methylene blue. III. Alkali methods of polychroming. Stain Technol. 1943a; 18: 1–11
  • Lillie R. D. A Giemsa stain of quite constant composition and performance, made in the laboratory from eosin and methylene blue. Publ. Health Rep. 1943b; 55: 440–452
  • Lillie R. D. Factors influencing the Romanowsky staining of blood films and the role of methylene violet. J. Lab. Clin. Med. 1944; 29: 1181–1197
  • Lillie R. D., Donaldson P. T. Zinc chloride methylene blue. I. Biological stain history, physical characteristics and approximation of azure B content of commercial samples. Stain Technol. 1979a; 54: 33–39
  • Lillie R. D., Donaldson P. T. The wet Giemsa method for quick testing of variants in blood and malaria stains. Stain Technol. 1979b; 54: 47–48
  • Lillie R. D., Donaldson P. T., Pizzolato P. Preparation of eosinates and Giemsa stains of low azure B content from dichromate oxidized commercial medicinal methylene blue. Stain Technol. 1978; 53: 337–343
  • Lillie R. D., Lindner J. Romanowsky Malachowski stains: Malachowski's 1891 use of alkali polychromed methylene blue for malaria plasmodia. Stain Technol. 1978; 53: 23–28
  • Lillie R. D., Roe M. A. Studies on polychrome methylene blue. I. Eosinates: their spectra and staining capacity. Stain Technol. 1942; 17: 57–63
  • Malachowski E. Zur Morphologie des Plasmodium malariae. Cbl. Klin. Med. 1891; 12: 601–603
  • Nocht B. Zur Färburg der Malariaparasiten. Cbl. Bakt. (Orig.) 1898; 24: 839–843
  • Reuter K. Über den färbenden Bestandteil der Romanowsky-Nochtschen Malariaplasmodien-färbung, sein Reindarstellung und praktische Venvendung. Zbl. Bakt. (Orig.) 1901; 30: 248–256
  • Roe M. A., Lillie R. D., Wilcox A. American azures in preparation of satisfactory Giemsa stains for malaria. Public Health Rep. 1940; 55: 1272–1273
  • Russo A., Donaldson P. T., Lillie R. D. Lower azure B methylene blue ratios in Giemsa type blood and malaria stains. Stain Technol. 1978; 53: 37–41
  • Wright J. H. A rapid method of staining blood and malarial parasites. J. Med. Res. 1902; 7: 138–144

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