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Original Article

Immunofluorescent Labelling of K-Papovavirus Antigens in Glycol Methacrylate Embedded Material: A Method for Studying Infected Cell Populations by Fluorescence Microscopy and Histological Staining of Adjacent Sections

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Pages 197-205 | Published online: 12 Jul 2009

References

  • Ashford A. E., Allaway W. G., McCully M. E. Low temperature embedding in glycol methacrylate for enzyme histochemistry in plant and animal tissues. J. Histochem. Cytochem 1972; 20: 986–990
  • Ashley C. A., Feder N. Glycol methacrylate in histopathology. Arch. Path 1966; 81: 391–397
  • Bennett H. S., Wyrick A. D., Lee S. W., McNeil J. H. Science and art in preparing tissues embedded in plastic for light microscopy, with special reference to glycol methacrylate, glass knives, and simple stains. Stain Technol 1976; 51: 71–97
  • Brinn N. T., Pickett J. P. Glycol methacrylate for routine, special stains, histochemistry, enzyme histochemistry and immunohistochemistry. J. Histotechnol 1979; 2: 125–130
  • Budka H., Popow-Kraupp T. Immunohistological studies in viral encephalitis. Acta Neuropathol. (Berl.) Suppl VII: 142-144. 1981
  • Carnegie J. A., McCully M. E., Robertson H. A. Embedment in glycol methacrylate at low temperature allows immunofluorescent localization of a labile tissue protein. J. Histochem. Cytochem 1980; 28: 308–310
  • Craig S., Goodchild D. J., Millerd A. Immunofluorescent localization of pea storage proteins in glycol methacrylate embedded tissue. J. Histochem. Cytochem 1979; 27: 1312–1316
  • Greenlee J. E. Pathogenesis of K virus infection in newborn mice. Infec. Immun 1979; 26: 705–713
  • Greenlee J. E. Effect of host age on experimental K virus infection in mice. Infec. Immun 1981; 33: 297–303
  • Huang S., Minassian H., Moore J. D. Application of immunofluorescent staining on paraffin sections improved by trypsin digestion. Lab. Invest 1976; 35: 383–390
  • Kawamura A. Fluorescent Antibody Techniques and Their Application, 2nd ed. University of Tokyo Press. 1977; 66
  • Kuhlmann W. D. Cytological localization of antigens: tissue fixation and processing in immunoenzyme techniques. First International Symposium on Immunoenzymatic Techniques, G. Feldman. North Holland Publishing Company, Amsterdam 1976; 91–98
  • Kumanishi T., Hirano A. An immunoperoxidase study on herpes simplex virus encephalitis. J. Neuropathol. Exp. Neurol 1978; 37: 790–795
  • Lohler J. Immunohistochemical demonstration of viral antigens in paraffin-embedded autopsy specimens of virally infected central nervous system. Acta Neuropathol. (Berl.) Suppl. VII: 139-141. 1981
  • Manual of Staining Methods of the Armed Forces Institute of Pathology, 3rd ed, L. G. Luna. McGraw-Hill, New York 1968; 35–37
  • Malm M. p-Toluenesulfonic acid as a fixative. Q. J. Microsc. Sci 1962; 103: 163–171
  • Margolis G., Kilham L., Gonats N. K. Reovirus type III encephalitis: observations of virus-cell interactions in neural tissues. Lab. Invest 1971; 24: 91–100
  • Schmidt N. J. Tissue culture techniques for diagnostic virology. Diagnostic Procedures for Viral and Rickettsial Infections, 4th ed, E. H. Lennette. American Public Health Association, New York 1969; 79–178
  • Stemberger L. A. Immunocytochemistry, 2nd ed. Wiley, New York 1979; 104–170
  • Swoveland P. T., Johnson K. P. Enhancement of fluorescent antibody staining of viral antigens in formalin-fixed tissues by trypsin digestion. J. Infect. Dis 1979; 140: 758–764
  • Van de Velde S. Fluorescent microscopy of glycol methacrylate-embedded tissue. Am. J. Clin. Pathol 1980; 73: 119–121

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