21
Views
1
CrossRef citations to date
0
Altmetric
Original Article

Observations on the Kinetics of Uranyl Acetate and Phosphotungstic Acid Staining of Chromatin in thin Sections for Electron Microscopy

&
Pages 291-304 | Published online: 12 Jul 2009

References

  • Bloch D. P. A catalog of sperm histones. Genetics Suppl. 1969; 61: 93–111
  • Burge R. E., Silvester N. R. The measurement of mass, thickness, and ty in the electron microscope. J. Biophys. Biochem. Cytol. 1960; 8: 1–11
  • Gattini P. A. tudies on the distribution of DNA and histones in the higher order fibre of chromatin. Ph.D. Thesis, University of London. 1983
  • Cattini P. A., Davies H. G. Kinetics of lead citrate staining of thin sections for electron microscopy. Stain Technol. 1983; 58: 29–40
  • Cosslett V. E. Quantitative aspects of electron staining. J. Roy. Microsc. Soc. 1958; 78: 18–25
  • Courtens J. L., Loir M. Ultrastructural detection of basic nucleoproteins: alcoholic phosphotungstic acid does not bind to arginine residues. J. Ultrastruct. Res. 1981; 74: 322–326
  • Davies H. G. Electron microscopy of interphase chromosomes in situ: binding of permanganate to chicken erythrocytes. J. Cell Sci. 1976; 20: 289–308
  • Davies H. G., Murray B., Walmsley M. E. Electron microscope observations on the organisation of the nucleus in chicken erythrocytes and a superunit thread hypothesis for chromosome structure. J. Cell Sci. 1974; 16: 261–299
  • Hall C. E. Electron densitometry of stained virus particles, J. Biophys. Biochem. Cytol. 1955; 1: 1–12
  • Hayat M. A. Positive Staining for Electron Microscopy. Van Nostrand Reinhold, New York 1975
  • Hayat M. A. Fixation for Electron Microscopy. Academic Press, New York 1981
  • Horobin R. W. Staining plastic sections: a review of problems, explanations and possible solutions. J. Microsc. 1983; 131: 173–186
  • Horobin R. W., Tomlinson A. The influence of the embedding medium when staining for electron microscopy: the penetration of stains into plastic sections. J. Microsc. 1976; 108: 69–78
  • Lewis P. R., Knight D. P. Practical Methods in Electron Microscopy. North-Holland Publishing Company, Amsterdam 1977; Vol. 5, Part 1
  • Pearson E. C. Light and electron microscopy observations on recently observed nucleolar-like organelles, and their possible origin, in certain differentiated cells. Ph.D. Thesis, University of London. 1980
  • Pearson E. C., Davies H. G. A critical evaluation of Bernhard's EDTA regressive staining technique for RNA. J. Cell Sci. 1982; 54: 207–240
  • Reynolds E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 1963; 17: 208–212
  • Richardson W. D. Quantitative and qualitative electron microscope observations on condensed nucleoprotein in avian erythrocytes, and in spermatids of a mollusc, Nucella lapillus. Ph.D. Thesis, University of London. 1978
  • Richardson W. D., Davies H. G. Quantitative observations on the kinetics and mechanisms of binding of electron stains to thin sections through hen erythrocytes. J. Cell Sci. 1980; 46: 253–278
  • Sabatini D. D., Bensch K., Barrnett R. J. Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymic activity by aldehyde Fixation. J. Cell Biol. 1963; 17: 19–58
  • Small J. V. Measurement of section thickness. Proc. 4th Eur. eg. Conf. Electron Microsc. 1968, D. S. Bocciarelli. Vol 1: 609–610
  • Spurr A. R. A low viscosity epoxy resin embedding medium for electron microscopy. J. Ultrastruct. Res. 1969; 26: 31–43
  • Tooze J. Measurement of some cellular changes during fixation of amphibian erythrocytes with osmium tetroxide solutions, J. Cell Biol. 1964; 22: 551–563
  • Walker M., Macgregor H. C. Spermatogenesis and the structure of the mature sperm in Nucella lapillus. J. Cell Sci. 1968; 3: 95–104
  • Zeitler E., Bahr G. F. Contrast and mass thickness. Lab. Invest. 1965; 14: 946–954

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.