Evaluation of DAPI as a Fluorescent Probe for DNA in Viable Petunia Protoplasts

References

• Amino S-I., Fujimura T., Komamine A. Synchrony induced by double phosphate starvation in a suspension culture of Catharanthus roseus. Physiol. Plant 1983; 59: 393–396
   Google Scholar
• Arndt-Jovin D. J., Jovin T. M. Analysis and sorting of living cells according to deoxyribonucleic acid content. J. Histochem. Cytochem 1977; 25: 585–589
   PubMed Web of Science ®Google Scholar
• Ausubel F. M., Bahnsen K., Hanson M., Mitchell A., Smith H. J. Cell and tissue culture of haploid and diploid Petunia “Mitchell.” Plant Mol. Biol 1980; 1: 26–32
   Google Scholar
• Bergounioux C., Perennes C., Miege C., Gadal P. The effect of male sterility on protoplast division in Petunia hybrida. Cell cycle comparison by flow cytometry. Protoplasma 1986; 130: 138–144
   Google Scholar
• Conia J., Alexander M. E., Richards K. R., Rice M. E., Jackson P. J. Reversible accumulation of plant suspension cell cultures in G1 phase and subsequent synchronous traverse of the cell cycle. Plant Physiol 1990; 94: 1568–1574
   PubMed Web of Science ®Google Scholar
• Coleman A. W., Goff J. M. Applications of fluorochromes to pollen biology. I. Mithramycin and 4′6-diamidino-2-phenylindole (DAPI) as vital stains and for quantitation of nuclear DNA. Stain Technol. 1985; 60: 145–154
   PubMed Web of Science ®Google Scholar
• Delaat, A A. M., Gohde W., Vogelzang M. J. D. C. Determination of ploidy of single plants and plant populations by flow cytometry. Plant Breeding 1987; 99: 303–307
   Google Scholar
• Dolezal J., Binarova P., Lucretti S. Analysis of nuclear DNA content in plant cells by flow cytometry. Biol. Plant 1989; 31: 113–120
   Google Scholar
• Erba E., Ubezio P., Broggini M., Ponti M., D'Incalci M. DNA damage, cytotoxic effect and cell-cyle perturbation of Hoechst- 33342 on L121 cells in vitro. Cytometry 1988; 9: 1–6
   PubMedGoogle Scholar
• Firoozabady E. The effects of cell cycle parameters on cell wall regeneration and cell division of cotton protoplasts (Gossypium hirsutum L.). J. Exp. Bot 1986; 37: 1211–1217
   Google Scholar
• Frearson E. M., Power J. B., Cocking E. C. The isolation. culture and regeneration of Petunia leaf protoplasts. Dev. Biol 1973; 33: 130–137
   PubMed Web of Science ®Google Scholar
• Fried J., Doblin J., Takomoto S., Perez A, Hansen H., Clarkson B. Effects of Hoechst 33342 on survival and growth of two tumor cell lines and on hematopoietically normal bone marrow cells. Cytometry 1982; 3: 42–47
   PubMedGoogle Scholar
• Galbraith D. W. Flow cytometric analysis of the cell cycle. Cell Culture and Somatic Cell Genetics of Plants, I. K. Vasil. Academic Press, New York 1984; Vol. I: 765–777
   Google Scholar
• Galbraith D. W., Mauch T. J., Shields B. A. Analysis of the initial stages of plant protoplast development using 33258 Hoechst: reactivation of the cell cycle. Physiol. Plant 1981; 51: 380–386
   Web of Science ®Google Scholar
• Galbraith D. W., Harkins K. R., Maddox J. M., Ayres N. M., Sharma D. P., Firoozabady E. Rapid flow cytometric analysis of the cell cycle in intact plant tissues. Science 1983; 220: 1049–1051
   PubMed Web of Science ®Google Scholar
• Gregoire M., Hernandez-Verdun D., Bouteille M. Visualization of chromatin distribution in living PTO cells by Hoechst 33342 fluorescent staining. Exp. Cell Res 1984; 152: 38–46
   PubMedGoogle Scholar
• Guri A., Zelcer A., Izhar S. Induction of high mitotic index in Petunia suspension cultures by sequential treatment with aphidicolin and colchicine. Plant Cell Rep 1984; 3: 219–221
   PubMed Web of Science ®Google Scholar
• Hamori E., Arndt-Jovin D. J., Grimwade B. G., Jovin T. M. Selection of viable cells with known DNA content. Cytometry 1980; 1: 132–146
   PubMedGoogle Scholar
• Hansen M. R., Kook A. J. Molecular biology, K. C. Petunia Sink. Springer Verlag, Heidelberg 1984; 155
   Google Scholar
• Latt S. A. Fluorescent probes of chromosome structure and replication. Can. J. Genet. Cytol 1977; 19: 603–623
   PubMedGoogle Scholar
• McClintock B. A method for making acetocarmine smears permanent. Stain Technol 1929; 4: 53–56
   Google Scholar
• Meadows M. G. Characterization of cells and protoplasts of the 873 maize cell line. Plant Sci. Lett 1983; 28: 337–348
   Google Scholar
• Meadows M. G., Potrykus I. Hoechst 33258 as a vital stain for plant cell protoplasts. Plant Cell Rep 1981; 1: 77–79
   PubMedGoogle Scholar
• Meyer P., Walgenbach E., Bussmann K., Hombrecher G., Saedler H. Synchronized tobacco protoplasts are efficiently transformed by DNA. Mol. Gen. Genet 1985; 201: 513–518
   Google Scholar
• Murashige T., Skoog F. A revised medium for rapid growth and bio assavs with tobacco tissue cultures. Physiol. Plant 1962; 15: 473–497
   Web of Science ®Google Scholar
• Nishinari N., Syono K. Induction of cell division synchrony and variation of cytokinin contents through the cell cycle in tobacco cultured cells. Plant Cell Physiol 1986; 27: 147–153
   Web of Science ®Google Scholar
• Patel R., Twigg J., Crossley I., Golsteyn R., Whitaker M. Calcium-induced chromatin condensation and cyclin phosphorylation during chromatin condensation cycles in ammonia-activated sea urchin eggs. J. Cell Sci. Suppl 1989; 12: 129–144
   PubMedGoogle Scholar
• Puite K. J., Ten Broeke W. W. R. DNA staining of fixed and non-fixed plant protoplasts for flow cytometry with Hoechst 33342. Plant Sci 1983; 32: 79–88
   Google Scholar
• Sala F., Calli M. G., Pedrali-Noy G., Spadari S. Synchronization of plant cells in culture and in meristems by aphidicolin. Methods in Enzymology, Vol. 118., A. Weissbach, H. Weissbach. Academic Press, New York 1986; 87–96
   Google Scholar
• Sharma C. B., Panoa B. B., Sahu R. K. A Feulgen-substituting rapid schedule for some plant chromosomes involving a new pretreating agent and macerating fixative. Mikroskopie 1977; 33: 30–34
   PubMedGoogle Scholar
• Ulrich I., Fritz B., Ulrich W. Application of DNA fluorochromes for flow cytometric DNA analysis of plant protoplasts. Plant Sci 1988; 55: 151–158
   Web of Science ®Google Scholar
• Ulrich I., Ulrich W. Flow cytometric DNA-analysis of plant protoplasts stained with DAPI. 2. Naturforsch. 41 Sect. C Biosci 1986; 1052–1056
   Google Scholar
• Van der Valk H. C.P. M., Blaas J., Van Eck J. W., Verhoeven H. A. Vital DNA staining of agarose-embedded protoplasts and cell suspensions of Nicotiana plumbaginifolia. Plant Cell Rep. 1988; 7: 489–492
   PubMedGoogle Scholar
• Vergne P., Delvallee I., Dumas C. Rapid assessment of microspore and pollen development stage in wheat and maize using DAPI and membrane permeabilization. Stain Technol 1987; 62: 299–304
   PubMed Web of Science ®Google Scholar
• Wang A. S., Phillips R. L., Mi C. C. Cell cycle parameters and accumulation of metaphase cells in maize suspension cultures. Plant Sci 1986; 46: 53–61
   Web of Science ®Google Scholar
• Weber G., DeGroot E., Schweizer H. G. Synchronization of protoplasts from Clycine max (L.) Merr. and Brassica napris (L.). Planta 1986; 168: 273–280
   PubMedGoogle Scholar
• Widholm J. M. The use of fluorescein diacetate and phenosafranine for determining viability of cultured plant cells. Stain Technol 1972; 47: 189–194
   PubMed Web of Science ®Google Scholar
• Williamson D. H., Fennell D. J. The use of fluorescent DNA-binding agents for detecting and separating yeast mitochondrial DNA. Methods in Cell Biology XII. Yeast Cells, Prescott. Academic Press, New York 1975; 335–351
   Google Scholar