386
Views
38
CrossRef citations to date
0
Altmetric
Original Article

The Role of DNase and EDTA on DNA Degradation in Formaldehyde Fixed Tissues

, , , , &
Pages 123-129 | Published online: 12 Jul 2009

References

  • Akiyoshi H. Studies on nuclear acid deoxyri-bonucleases and proofreading exonuclease isolated from rat liver nuclei. J. Biochem. 1988; 104: 521–525
  • Boseley P. G., Bradbury E. M., Butler-Browne G. S., Carpenter B. G., Stephens R. M. Physical studies of chromatin. The recombination of histones with DNA. Eur. J. Biochem. 1976; 62: 21–31
  • Brutlag D., Schlehuber C., Bonner J. Properties of formaldehyde-treated nucleohistone. Biochemistry 1969; 8: 3214–3218
  • Chang E. H., Gonda M. A., Ellis R. W., Scolnick E. M., Lowy D. R. Human genome contains four genes homologous to transforming genes of Harvey and Kirsten murine sarcoma viruses. Proc. Natl. Acad. Sci. (USA) 1982; 79: 4848–4852
  • Dubeau L., Chandler L. A., Gralow J. R., Nichols P. W., Jones P. A. Southern blot analysis of DNA extracted from formalin-fixed pathology specimens. Cancer Res. 1986; 46: 2964–2969
  • Goelz S. E., Hamilton S. R., Vogelstein B. Purification of DNA from formaldehyde fixed and paraffin embedded human tissue. Biochem. Biophys. Res. Commun. 1985; 130: 118–126
  • Green C. E., Peterson S. L., Kiviat N. B., Manos M. M. PCR amplification from paraffin-embedded tissues. Am. J. Clin. Pathol. 1991; 95: 117–124
  • Hanoch S., Tama L. Studies on the neutral deoxyribonuelease activity in highly purified acid deoxyribonuelease. J. Biol. Chem. 1972; 247: 2926–2930
  • Impraim C. C., Saiki R. K., Erlich H. A., Teplitz R. L. Analysis of DNA extracted from formalin-fixed, paraffin-embedded tissues by enzymatic amplification and hybridization with sequence-specific oligonucleotides. Biochem. Biophys. Res. Commun. 1987; 142: 710–716
  • Ishikawa J., Maeda S., Takahashi R., Kamidono S., Sugiyama T. Lack of correlation between rare Ha-ras alleles and urothelial cancer in Japan. Int. J. Cancer 1987; 40: 474–478
  • Kuykendall J. R., Bogdanffy M. S. Efficiency of DNA-histone crosslinking induced by saturated and unsaturated aldehydes in vitro. Mutat. Res. 1992; 283: 131–136
  • Louis D., Kenneth W., Peter A J., Peter W. N. Studies on immunoglobulin gene rearrangement in formalin-fixed, paraffin-embedded pathology specimens. Am. J. Pathol. 1988; 130: 588–594
  • Mies C., Houldsworth J., Chaganti R. S. K. Extraction of DNA from paraffin blocks for Southern blot analysis. Am. J. Surg. Pathol. 1991; 15: 169–174
  • Nuovo G. J., Silverstein S. J. Comparison of formalin, buffered formalin, and Bouin's fixation on the detection of human papilloma virus deoxyribonucleic acid from genital lesions. Lab. Invest. 1988; 59: 720–724
  • Rogers B. B., Alpert L. C., Hine E. A. S., Buffone G. J. Analysis of DNA in fresh and fixed tissue by the polymerase chain reaction. Am. J. Pathol. 1990; 136: 541–548
  • Shibata D. K., Arnheim N., Martin W. J. Detection of human papilloma virus in paraffin-embedded tissue using the polymerase chain reaction. J. Exp. Med. 1988; 167: 225–230
  • Slor H., Lev T. Acid deoxyribonuelease activity in purified calf thymus nuclei. Biochem. J. 1971; 123: 993–995
  • Stephen A L., Jean-Louis D., Shu-Uin Y. Deoxyribonucleic acid-protein and deoxyribonucleic acid interstrand cross-links induced in isolated chromatin by hydrogen peroxide and ferrous ethylenediaminetetraacetate chelates. Biochemistry 1982; 21: 5010–5015
  • Tokuda Y., Nakamura T., Satonaka K., Maeda S., Doi K., Baba S., Sugiyama T. Fundamental study on the mechanism of DNA degradation in tissues fixed in formaldehyde. J. Clin. Pathol. 1990; 43: 748–751
  • Wan J. H., Trainor K. J., Brisco M. J., Morley A. A. Monoclonality in B cell lymphoma detected in paraffin wax embedded sections using the polymerase chain reaction. J. Clin. Pathol. 1990; 43: 888–890

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.