33
Views
3
CrossRef citations to date
0
Altmetric
Original Article

Hematoxylin Procedure for Staining Mature Pollen Grains in Maize with Dimethylsulfoxide as a Clearing Agent

Pages 1-5 | Received 25 Oct 1996, Accepted 10 Aug 1997, Published online: 12 Jul 2009

References

  • Chang M. T., Neuffer M. G. A simple method for staining nuclei of mature and germinated maize pollen. Stain Technol. 1989a; 64: 181–184
  • Chang M. T., Neuffer M. G. Maize microsporogenesis. Genome 1989b; 32: 232–244
  • Chang M. T., Neuffer M. G. Position of the vegetative and sperm cells of germinating pollen grain in maize (Zea mays L.). Maydica 1992; 37: 233–243
  • Kato A. Induction of bicellular pollen and dihaploidization of tetraploid maize. Maize Genet. Coop. Newslett. 1996; 70: 25–26
  • Kato A. Induced single fertilization in maize. Sex. Plant Reprod. 1997; 10: 96–100
  • Kindiger B., Beckett J. B. A hematoxylin staining procedure for maize pollen grain chromosomes. Stain Technol. 1985; 60: 265–269
  • McConchie C. A., Hough T., Knox R. B. Ultrastructural analysis of the sperm cells of mature pollen of maize, Zea mays. Protoplasma 1987; 139: 9–19
  • Sallee P. J., Kimber G. The use of DMSO in the prefixation of somatic chromosomes. Cereal Res. Commun. 1981; 9: 199–203
  • Sallee P. J. Prefixation and staining of the somatic chromosomes of corn. Maize for Biological Research., W. F. Sheridan. University Press, University of North Dakota. 1982; 119–120
  • Thiebaut J., Kasha K. J. Modification of the colchicine technique for chromosome doubling of barley haploids. Can. J. Genet. Cytol. 1978; 20: 513–521
  • Vergne P., Delvallee I., Dumas C. Rapid assessment of microspore and pollen development stage in wheat and maize using DAPI and membrane permeabilization. Stain Technol. 1987; 62: 299–304

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.