36
Views
0
CrossRef citations to date
0
Altmetric
Original Article

Improved Osmication, Dehydration and Flat Embedding of Vibratome Sections and Delicate Whole Mount Preparations

Pages 52-56 | Received 11 Mar 1997, Accepted 21 Nov 1997, Published online: 12 Jul 2009

References

  • Campbell R. D., Hermans C. O. A rapid method for re-sectioning 0.5–4 μm epoxy sections for electron microscopy. Stain Technol. 1972; 47: 115–118
  • Grimley P. M. Selection for electron microscopy of specific areas in large epoxy tissue sections. Stain Technol. 1965; 40: 259–263
  • Grimley P. M., Carleton J. H., Michelitch H. J., Priester J. L. Techniques of preparing large plastic sections for conjugate light and electron microscopy. J. Cell Biol. 1966; 31: 130A
  • Migheli A., Attanosio A. Two methods for flat embedding sections in LR White cut from paraffin blocks. Biotech. & Histochem. 1991; 1: 89–92
  • Parr M. B., Hunter S. A., Parr E. L. A method for flat embedding thick cryostat sections in plastic resin. Stain Technol. 1989; 64: 261–263
  • Reymond O. L., Pickett-Heaps J. D. A routine flat embedding method for electron microscopy of microorganisms allowing selection and precisely oriented sectioning of single cells by light microscopy. J. Microsc. 1982; 130: 79–84
  • Schäfer K.-H. Morphology of GABA-ergic neurons in the medial preoptic nucleus of the rat. Thesis, Medical Faculty, University of Saarland, HomburgGermany 1993
  • Schäfer K.-H., Lintz M., Booz K. H. Changes in the innervation of the portal vein during early postnatal development. Ann. Anat. 1997; 179(Suppl. 297)
  • Schwartz I. R. A simple technique for osmicating and flat embedding large tissue sections for light and electron microscopy. Stain Technol. 1982; 57: 52–54
  • Yu S. M., Schwartz I. An improved flat embedding technique for immunoelectron microscopy. Stain Technol. 1989; 64: 143–146

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.