Chromatographic methods for the analysis of oxidatively damaged DNA

References

• Ames BN, Gold LS. Endogenous mutagens and the causes of aging and cancer. Mutat Res 1991;250:3–16.
   PubMed Web of Science ®Google Scholar
• Ames BN, Gold LS, Willett WC. The causes and prevention of cancer. Proc Natl Acad Sci USA 1995;92:5258–5265.
   PubMed Web of Science ®Google Scholar
• Lindahl T. Instability and decay of the primary structure of DNA. Nature 1993;362:709–715.
   PubMed Web of Science ®Google Scholar
• Marnett LJ. Oxyradicals and DNA damage. Carcinogenesis 2000;21(3):361–370.
   PubMed Web of Science ®Google Scholar
• Aust AE, Eveleigh JF. Mechanisms of DNA oxidation. Proc Soc Exp Biol Med 1999;222(3):246–252.
   PubMed Web of Science ®Google Scholar
• Collins AR, Dusinska M, Gedik CM, Stetina R. Oxidative damage to DNA: Do we have reliable biomarker? Environ Health Perspect 1996;104(3):465–469.
   PubMedGoogle Scholar
• Ravanat J-L. Measuring oxidized DNA lesions as biomarkers of oxidative stress: An analytical challenge. FABAD, J Pharm Sci 2005;30(2):100–113.
   Google Scholar
• Cadet J, Douki T, Ravanat J-L. Measurement of oxidatively generated base damage in cellular DNA. Mutat Res 2011;711:3–12.
   PubMed Web of Science ®Google Scholar
• Cadet J, Douki T, Ravanat J-L. Oxidatively generated base damage to cellular DNA. Free Radic Biol Med 2010;49(1): 9–21.
   PubMed Web of Science ®Google Scholar
• Guetens G, De Boeck G, Highley M, van Oosterom AT, de Bruijn EA. Oxidative DNA damage: biological significance and methods of analysis. Crit Rev Clin Lab Sci 2002; 39(4–5):331–457.
   PubMed Web of Science ®Google Scholar
• Collins AR, Cadet J, Moller L, Poulsen HE, Vina J. Are we sure we know how to measure 8-oxo-7,8-dihydroguanine in DNA from human cells? Arch Biochem Biophys 2004;423(1): 57–65.
   PubMed Web of Science ®Google Scholar
• Cadet J, Douki T, Ravanat J-L. Artifacts associated with the measurement of oxidized DNA bases. Environ Health Perspect 1997;105(10):1033–1039.
   Web of Science ®Google Scholar
• Frenkel K, Goldstein MS, Teebor GW. Identification of the cis-thymine glycol moiety in chemically oxidized and gamma-irradiated deoxyribonucleic acid by high-pressure liquid chromatography analysis. Biochemistry 1981;20(26): 7566–7571.
   PubMed Web of Science ®Google Scholar
• Frenkel K, Grunberger D, Kasai H, Komura H, Nakanishi K. Identification of novel 7,12-dimethylbenz[a]anthracene adducts in cellular ribonucleic acid. Biochemistry 1981;20(15): 4377–4381.
   PubMed Web of Science ®Google Scholar
• Kasai H, Tanooka H, Nishimura S. Formation of 8-hydroxyguanine residues in DNA by X-irradiation. Gann 1984;75(12):1037–1039.
   PubMedGoogle Scholar
• Kasai H, Nishimura S. Hydroxylation of deoxyguanosine at the C-8 position by ascorbic acid and other reducing agent. Nucleic Acids Res 1984;12:2137–2145.
   PubMed Web of Science ®Google Scholar
• Floyd RA, Watson JJ, Wong PK. Sensitive assay of hydroxyl free radical formation utilizing high pressure liquid chromatography with electrochemical detection of phenol and salicylate hydroxylation products. J Biochem Biophys Methods 1984;10(3–4):221–235.
   PubMedGoogle Scholar
• Floyd RA, Watson JJ, Harris J, West M, Wong PK. Formation of 8-hydroxydeoxyguanosine, hydroxyl free radical adduct of DNA in granulocytes exposed to the tumor promoter, tetradecanoylphorbolacetate. Biochem Biophys Res Commun 1986;137(2):841–846.
   PubMed Web of Science ®Google Scholar
• Kasai H, Crain PF, Kuchino Y, Nishimura S, Ootsuyama A, Tanooka H. Formation of 8-hydroxyguanosine moiety in cellular DNA by agents producing oxygen radicals and evidence for its repair. Carcinogenesis 1986;7:1849–1851.
   PubMed Web of Science ®Google Scholar
• Kaur H, Halliwell B. Measurement of oxidized and methylated DNA bases by HPLC with electrochemical detection. Biochem J 1996;318:21–23.
   PubMed Web of Science ®Google Scholar
• Wagner JR, Hu CC, Ames BN. Endogenous oxidative damage of deoxycytidine in DNA. Proc Natl Acad Sci USA 1992;89:3380–3384.
   PubMed Web of Science ®Google Scholar
• Riviere J, Ravanat J-L, Wagner JR. Ascorbate and H2O2 induced oxidative DNA damage in Jurkat cells. Free Radic Biol Med 2006;40(12):2071–2079.
   PubMed Web of Science ®Google Scholar
• Bogdanov MB, Beal MF, McCabe DR, Griffin RM, Matson WR. A carbon column-based liquid chromatography electrochemical approach to routine 8-hydroxy-2′′-deoxyguanosine measurements in urine and other biologic matrices: a one-year evaluation of methods. Free Radic Biol Med 1999; 27(5–6):647–666.
   PubMed Web of Science ®Google Scholar
• Dizdaroglu M. The use of capillary gas chromatography-mass spectrometry for identification of radiation induced DNA bases damage and DNA base-amino acid cross-links. J. Chromatogr 1984;295:103–121.
   Google Scholar
• White E, Krueger VP, McCloskey JA. Mass spectra of trimethysilyl derivatives of pyridines and purine bases. J Org Chem 1972;37:430–438.
   PubMed Web of Science ®Google Scholar
• Dizdaroglu M. Formation of an 8-hydroxyguanine moiety in deoxyribonucleic acid on gamma irradiation in aqueous solution. Biochemistry 1985;24:4476–4481.
   PubMed Web of Science ®Google Scholar
• Teixeira AJR, Gommers-Ampt JH, van de Werken G, Westra JG, Stavenuiter JFC, de Jong PJM. Methods for the analysis of oxidized nucleosides by gas chromatography/mass spectrometry. Anal Chem 1993;214:474–483.
   Google Scholar
• Dizdaroglu M. Quantitative determination of oxidative base damage in DNA by stable isotope-dilution mass spectrometry. FEBS Lett 1993;315:1–6.
   PubMed Web of Science ®Google Scholar
• Ravanat J-L, Turesky RJ, Gremaud E, Trudel LJ, Stadler RH. Determination of 8-oxoguanine in DNA by gas chromatography-mass spectrometry and HPLC-electrochemical detection. Overestimation of the background level of the oxidized base by the gas chromatography-mass spectrometry assay. Chem Res Toxicol. 1995;8:1039–1045.
   PubMed Web of Science ®Google Scholar
• Halliwell B, Dizdaroglu M. Commentary. The measurement of oxidative damage to DNA by HPLC and GC/MS techniques. Free Radic. Res Commun 1992;16:75–87.
   Google Scholar
• Ravanat J-L, Gremaud E, Markovic J, Turesky RJ. Detection of 8-oxoguanine in cellular DNA using 2,6-diamino-8-oxopurine as an internal standard for HPLC-EC detection. Anal Biochem 1998;260(1):30–37.
   PubMed Web of Science ®Google Scholar
• Hamberg M, Zhang L-Y. Quantitative determination of 8-hydroxyguanine and guanine by isotope dilution mass spectrometry. Anal Biochem 1995;229:336–344.
   PubMed Web of Science ®Google Scholar
• Douki T, Delatour T, Bianchini F, Cadet J. Observation and prevention of an artifactual formation of oxidized DNA bases and nucleosides in the GC-EIMS method. Carcinogenesis 1996;17:347–353.
   PubMed Web of Science ®Google Scholar
• Boucher D, Testard I, Averbeck D. Low levels of clustered oxidative DNA damage induced at low and high LET irradiation in mammalian cells. Radiat Environ Biophys 2006; 45(4):267–276.
   PubMed Web of Science ®Google Scholar
• Rodriguez H, Jurado J, Laval J, Dizdaroglu M. Comparison of the levels of 8-hydroxyguanine in DNA as measured by gas chromatography mass spectrometry following hydrolysis of DNA by Escherichia coli Fpg protein or formic acid. Nucleic Acids Res. 2000;28(15):E75.
   PubMedGoogle Scholar
• Ravanat J-L, Berger M, Boiteux S, Laval J, Cadet J. Excision of 7,8-dihydro-8-oxoguanine from DNA by the Fpg protein. J. Chim. Phys. 1993;90:871–879.
   Google Scholar
• Bergeron F, Auvré F, Radicella JP, Ravanat J-L. HO° radicals induce an unexpected high proportion of tandem base lesions refractory to repair by DNA glycosylases. Proc Natl Acad Sci USA 2010;107(12):5528–5533.
   PubMed Web of Science ®Google Scholar
• Karakaya A, Jaruga P, Bohr VA, Grollman AP, Dizdaroglu M. Kinetics of excision of purine lesions from DNA by Escherichia coli Fpg protein. Nucleic Acids Res 1997;25(3):474–479.
   PubMed Web of Science ®Google Scholar
• Berger M, Cadet J, Berube R, Langlois R, van Lier JE. Reversed-phase high-performance liquid chromatography-thermospray mass spectrometry of radiation-induced decomposition of thymine and thymidine. J Chromatogr 1992;593:133–138.
   PubMed Web of Science ®Google Scholar
• Smith RD, Loo JA, Edmonds CG, Barinaga CJ, Udseth HR. New development in biochemical mass spectrometry: Electrospray ionisation. Anal Chem 1990;62:882–899.
   PubMed Web of Science ®Google Scholar
• Reddy DM, Iden CR. Analysis of modified deoxynucleosides by electrospray ionization mass spectrometry. Nucleosides Nucleotides 1993;12:815–826.
   Web of Science ®Google Scholar
• Richling E, Herderich M, Schreier P. High performance liquid chromatography - electrospray tandem mass spectrometry (HPLC-ESI-MS-MS) for the analysis of heterocyclic aromatic amines (HAA). Chromatographia 1996;42:7–11.
   Web of Science ®Google Scholar
• Weir Lipton W, Fuciarelli AF, Springer DL, Edmonds CG. Characterization of radiation-induced thymine-tyrosine crosslinks by electrospray ionization mass spectrometry. Radiat Res 1996;145(6):681–386.
   PubMed Web of Science ®Google Scholar
• Müller M, Belas FJ, Blair IA, Guengerich FP. Analysis of 1, N2-ethenoguanine and 5,6,7,9-tetrahydro-7-hydroxy-9-oxoimidazo[1,2a]purine in DNA treated with 2-chlorooxirane by high performance liquid chromatography/electrospray mass spectrometry and comparison of amounts of other dna adducts. Chem Res Toxicol 1997;10:242–247.
   PubMed Web of Science ®Google Scholar
• Serrano J, Palmeira CM, Wallace KB, Kuehl DW. Determination of 8-hydroxyguanosine in biological tissues by liquid chromatography/Electrospray ionization-mass spectrometry/mass spectrometry. Rapid Commun Mass Spectrom 1996; 10:1789–1791.
   PubMed Web of Science ®Google Scholar
• Ravanat J-L, Duretz B, Guiller A, Douki T, Cadet J. Isotope dilution high-performance liquid chromatography-electrospray tandem mass spectrometry assay for the measurement of 8-oxo-7,8-dihydro-2′-deoxyguanosine in biological samples. J. Chromatogr B 1998;715(2):349–356.
   PubMedGoogle Scholar
• Bessette EE, Spivack SD, Goodenough AK, Wang T, Pinto S, Kadlubar FF, Turesky RJ. Identification of carcinogen DNA adducts in human saliva by linear quadrupole ion trap/multistage tandem mass spectrometry. Chem Res Toxicol 2010;237:1234–1244.
   Google Scholar
• Regulus P, Duroux B, Bayle P-A, Favier A, Cadet J, Ravanat J-L. Oxidation of the sugar moiety of DNA by ionizing radiation or bleomycin could induce the formation of a cluster DNA lesion. Proc Natl Acad Sci, USA 2007;104(35):14032–14037.
   PubMed Web of Science ®Google Scholar
• Frelon S, Douki T, Ravanat J-L, Pouget J-P, Tornabene C, Cadet J. High performance liquid chromatography - tandem mass spectrometry for the measurement of radiation-induced base damage to isolated and cellular DNA. Chem Res Toxicol. 2000;13(10):1002–1010.
   PubMed Web of Science ®Google Scholar
• Mamer OA. Stable isotope dilution techniques. J. Chromatogr. B 1996;682(1):182–183.
   Google Scholar
• Weimann A, Belling D, Poulsen HE. Quantification of 8-oxo-guanine and guanine as the nucleobase, nucleoside and deoxynucleoside forms in human urine by high-performance liquid chromatography-electrospray tandem mass spectrometry. Nucleic Acids Res 2002;30(2):E7.
   PubMed Web of Science ®Google Scholar
• Singh R, McEwan M, Lamb JH, Santella RM, Farmer PB. An improved liquid chromatography/tandem mass spectrometry method for the determination of 8-oxo-7,8-dihydro-2′-deoxyguanosine in DNA samples using immunoaffinity column purification. Rapid Commun Mass Spectrom 2003;17(2):126–134.
   PubMed Web of Science ®Google Scholar
• Boysen G, Collins LB, Liao S, Luke AM, Pachkowski BF, Watters JL, Swenberg JA. Analysis of 8-oxo-7,8-dihydro-2′-deoxyguanosine by ultra high pressure liquid chromatography-heat assisted electrospray ionization-tandem mass spectrometry. J Chromatogr B 2010;878(3–4):375–380.
   PubMed Web of Science ®Google Scholar
• Regulus P, Spessotto S, Gateau M, Cadet J, Favier A, Ravanat J-L. Detection of new radiation-induced DNA lesions by liquid chromatography coupled to tandem mass spectrometry. Rapid Commun Mass Spectrom 2004;18(19): 2223–2228.
   PubMed Web of Science ®Google Scholar
• Maurel V, Ravanat J-L, Gambarelli S. Detection of reactive free radicals derived from nucleosides by liquid chromatography coupled to tandem mass spectrometry of DMPO spin trapping adducts. Rapid Commun Mass Spectrom 2006; 20(15):2235–2242.
   PubMed Web of Science ®Google Scholar
• Mouret S, Charveron M, Favier A, Cadet J, Douki T. Differential repair of UVB-induced cyclobutane pyrimidine dimers in cultured human skin cells and whole human skin. DNA Repair (Amst) 2008;7(5):704 –712.
   PubMed Web of Science ®Google Scholar
• Mouret S, Baudouin C, Charveron M, Favier A, Cadet J, Douki T. Cyclobutane pyrimidine dimers are predominant DNA lesions in whole human skin exposed to UVA radiation. Proc Natl Acad Sci USA 2006;103(37):13765–13770.
   PubMed Web of Science ®Google Scholar
• Hofer T, Badouard C, Dajak E, Ravanat J-L, Mattsson A, Cotgreave IA. Hydrogen peroxide causes greater oxidation in cellular RNA than in DNA. Biol Chem 2005;386: 333–337.
   PubMed Web of Science ®Google Scholar
• Badouard C, Masuda M, Nishino H, Cadet J, Favier A, Ravanat J-L. Detection of chlorinated DNA and RNA nucleosides by HPLC coupled to tandem mass spectrometry as potential biomarkers of inflammation. J Chromatogr B 2005; 827:26–31.
   PubMed Web of Science ®Google Scholar
• Masuda M, Suzuki T, Friesen MD, Ravanat J-L, Cadet J, Pignatelli B, . Chlorination of guanosine and other nucleosides by hypochlorous acid and myeloperoxidase of activated human neutrophils. J Biol Chem 2001;276(44): 40486–40496.
   PubMed Web of Science ®Google Scholar
• Meador JA, Su Y, Ravanat J-L, Balajee AS. DNA dependent protein kinase (DNA-PK) deficient human glioblastoma cells are preferentially sensitized by Zebularine. Carcinogenesis 2010;31:184–191.
   PubMed Web of Science ®Google Scholar
• Charles M-P, Ravanat J-L, Adamski D, D'Orazi G, Cadet J, Favier A, . N6-methyldeoxyadenosine, a nucleoside commonly found in prokaryotes, induces C2C12 myogenic differentiation. Biochem Biophys Res Commun 2004;314:476–482.
   Google Scholar
• Ratel D, Ravanat J-L, Charles MP, Platet N, Breuillaud L, Lunardi J, . Undetectable levels of N6-methyl adenine in mouse DNA: Cloning and analysis of PRED28, a gene coding for a putative mammalian DNA adenine methyltransferase. FEBS Lett 2006;580(13):3179–3184.
   PubMed Web of Science ®Google Scholar
• Pronier E, Almire C, Mokrani H, Vasanthakumar A, Simon A, da Costa Reis Monte Mor B, . Inhibition of TET2-mediated conversion of 5-methylcytosine to 5-hydroxymethylcytosine disturbs erythroid and granulo-monocytic differentiation of human hematopoietic progenitors. Blood 2011;118(9):2551–2555.
   PubMed Web of Science ®Google Scholar
• Tarantini A, Maitre A, Lefebvre E, Marques M, Marie C, Ravanat J-L, Douki T. Relative contribution of DNA strand breaks and DNA adducts to the genotoxicity of benzo[a]pyrene as a pure compound and in complex mixtures. Mutat Res 2009;671(1–2):67–75.
   PubMed Web of Science ®Google Scholar
• Marie C, Maitre A, Douki T, Gateau M, Tarantini A, Guiraud P, . Influence of the metabolic properties of human cells on the kinetic of formation of the major benzo[a]pyrene DNA adducts. J Applied Toxicol 2007;I28(5):579–590.
   Google Scholar
• Gangl ET, Turesky RJ, Vouros P. Determination of in vitro- and in vivo-formed DNA adducts of 2-amino-3-methylimidazo [4,5-f]quinoline by capillary liquid chromatography/microelectrospray mass spectrometry. Chem Res Toxicol 1999; 12(10):1019–1027.
   PubMed Web of Science ®Google Scholar
• Farmer PB, Brown K, Tompkins E, Emms VL, Jones DJ, Singh R, Phillips DH. DNA adducts: Mass spectrometry methods and future prospects. Toxicol Appl Pharmacol 2005;207(2 Suppl):293–301.
   PubMedGoogle Scholar
• Singh R, Sweetman GM, Farmer PB, Shuker DE, Rich KJ. Detection and characterization of two major ethylated deoxyguanosine adducts by high performance liquid chromatography, electrospray mass spectrometry, and 32P-postlabeling. Development of an approach for detection of phosphotriesters. Chem Res Toxicol 1997;10(1):70–77.
   PubMed Web of Science ®Google Scholar
• Martinez GR, Medeiros MHG, Ravanat J-L, Cadet J, Di Mascio P. [18O]-labeled singlet oxygen as a tool for mechanistic studies of 8-oxo-7,8-dihydroguanine oxidative damage: detection of spiroiminodihydantoin, imidazolone and oxazolone derivatives. Biol Chem 2002;383:607–617.
   PubMed Web of Science ®Google Scholar
• Martinez GR, Ravanat J-L, Cadet J, de Medeiros MH, Di Mascio P. Spiroiminodihydantoin nucleoside formation from 2′-deoxyguanosine oxidation by [(18)O-labeled] singlet molecular oxygen in aqueous solution. J Mass Spectrom 2007;42(10):1326–1332.
   PubMed Web of Science ®Google Scholar
• Evans MD, Singh R, Mistry V, Farmer PB, Cooke MS. Analysis of urinary 8-oxo-7,8-dihydro-2′′-deoxyguanosine by liquid chromatography-tandem mass spectrometry. Methods Mol Biol 2010;610:341–351.
   PubMedGoogle Scholar
• Evans MD, Saparbaev M, Cooke MS. DNA repair and the origins of urinary oxidized 2′′-deoxyribonucleosides. Mutagenesis 2010;25(5):433–442.
   PubMed Web of Science ®Google Scholar
• Marie C, Ravanat J-L, Badouard C, Marques M, Balducci F, Maitre A. Urinary levels of oxidative DNA and RNA damage among workers exposed to polycyclic aromatic hydrocarbons in silicon production: comparison with 1-hydroxypyrene. Environ Mol Mutagen 2009;50(2):88–95.
   PubMed Web of Science ®Google Scholar
• Weimann A, Belling D, Poulsen HE. Measurement of 8-oxo-2′-deoxyguanosine and 8-oxo-2′-deoxyadenosine in DNA and human urine by high performance liquid chromatography-electrospray tandem mass spectrometry. Free Radic Biol Med 2001;30(7):757–764.
   PubMed Web of Science ®Google Scholar
• Ravanat J-L, Guicherd P, Tuce Z, Cadet J. Simultaneous determination of five oxidative DNA lesions in human urine. Chem Res Toxicol 1999;12(9):802–808.
   PubMed Web of Science ®Google Scholar
• Pouget J-P, Frelon S, Ravanat J-L, Testard I, Odin F, Cadet J. Formation of modified DNA bases in cells exposed either to gamma radiation or to high-LET particles. Radiat Res 2002;157:589–595.
   PubMed Web of Science ®Google Scholar
• Teebor GW, Frenkel K, Goldstein MS. Ionizing radiation and tritium transmutation both cause formation of 5-hydroxymethyl-2′-deoxyuridine in cellular DNA. Proc Natl Acad Sci USA 1984;81:318–321.
   PubMed Web of Science ®Google Scholar
• Randerath K, Reddy MV, Gupta RC. 32P-labeling test for DNA damage. Proc Natl Acad Sci USA 1981;78(10): 6126–6129.
   PubMed Web of Science ®Google Scholar
• Reddy MV, Gupta RC, Randerath K. 32P-base analysis of DNA. Anal Biochem 1981;117(2):271–279.
   PubMed Web of Science ®Google Scholar
• Randerath K, Randerath E, Agrawal HP, Gupta RC, Schurdak ME, Reddy MV. Postlabeling methods for carcinogen-DNA adduct analysis. Environ Health Perspect 1985;62:57–65.
   PubMed Web of Science ®Google Scholar
• Reddy MV, Randerath K. 32P-postlabeling assay for carcinogen-DNA adducts: nuclease P1-mediated enhancement of its sensitivity and applications. Environ Health Perspect 1987;76:41–47.
   PubMed Web of Science ®Google Scholar
• Mouret J-F, Odin F, Polverelli M, Cadet J. 32P-postlabeling measurement of adenine N-1-oxide in cellular DNA exposed to hydrogen peroxide. Chem Res Toxicol 1990;3:102–110.
   PubMed Web of Science ®Google Scholar
• , Devanaboyina U-sGupta SP. Sensitive detection of 8-hydroxy-2′-deoxyguanosine in DNA by 32P-postlabeling assay and the basal levels in rat tissues. Carcinogenesis 1996;17(5): 917–924.
   PubMed Web of Science ®Google Scholar
• Lu L-JW, Tasaka F, Hokanson JA, Kohda K. Detection of 8-hydroxy-2′-deoxyguanosine in deoxyribonucleic acid by the 32P-postlabeling method. Chem Pharm Bull 1991;39(7): 1880–1882.
   PubMed Web of Science ®Google Scholar
• Lutgerink JT, de Graaf E, Hoebee B, Stavenuitez HFC, Westra JG, Kriek E. Detection of 8-hydroxyguanine in small amounts of DNA by 32P postlabeling. Anal Biochem 1992; 201:127–133.
   PubMed Web of Science ®Google Scholar
• Schuler D, Otteneder M, Sagelsdorff P, Eder E, Gupta RC, Lutz WK. Comparative analysis of 8-oxo-2′-deoxyguanosine in DNA by 32P- and 33P-postlabeling and electrochemical detection. Carcinogenesis 1997;18(12):2367–2371.
   PubMed Web of Science ®Google Scholar
• Weinfeld M, Soderlind K-JM. 32P-postlabeling detection of radiation-induced DNA damage: identification and estimation of thymine glycols and phosphoglycolate termini. Biochemistry 1991;30:1091–1097.
   PubMed Web of Science ®Google Scholar
• Moller L, Hofer T. [32P]ATP mediates formation of 8-hydroxy-2′-deoxyguanosine from 2′′-deoxyguanosine, a possible problem in the 32P-poslabling assay. Carcinogenesis 1997;18(12):2415–2419.
   PubMed Web of Science ®Google Scholar
• Zeisig M, Hofer T, Cadet J, Moller L. 32P-postlabeling high-performance liquid chromatography (32P-HPLC) adapted for analysis of 8-hydroxy-2′-deoxyguanosine. Carcinogenesis 1999;20(7):1241–1245.
   PubMed Web of Science ®Google Scholar
• Douki T, Martini R, Ravanat J-L, Turesky RJ, Cadet J. Measurement of 2,6-diamino-4-hydroxy-5-formamidopyrimidine and 8-oxo-7,8-dihydroguanine in isolated DNA exposed to gamma radiation in aqueous solution. Carcinogenesis 1997; 18(12):2385–2391.
   PubMed Web of Science ®Google Scholar
• Ravanat J-L, Remaud G, Cadet J. Measurement of the main photooxidation products of 2′-deoxyguanosine using chromatographic methods coupled to mass spectrometry. Arch Biochem Biophys 2000;374(2):118–127.
   PubMed Web of Science ®Google Scholar
• Falcone JM, Box HC. Selective hydrolysis of damaged DNA by nuclease P1. Biochim Biophys Acta 1997;1337(2):267–275.
   PubMedGoogle Scholar
• Belmadoui N, Boussicault F, Guerra M, Ravanat J-L, Chatgilialoglu C, Cadet J. Radiation-induced formation of purine 5′,8-cyclonucleosides in isolated and cellular DNA: high stereospecificity and modulating effect of oxygen. Org Biomol Chem 2010;8(14):3311–3219.
   Web of Science ®Google Scholar
• Jaruga P, Theruvathu J, Dizdaroglu M, Brooks PJ. Complete release of (5′′S)-8,5′′-cyclo-2′-deoxyadenosine from dinucleotides, oligodeoxynucleotides and DNA, and direct comparison of its levels in cellular DNA with other oxidatively induced DNA lesions. Nucleic Acids Res 2004;32(11):e87.
   PubMed Web of Science ®Google Scholar
• Ravanat J-L, Douki T, Duez P, Gremaud E, Herbert K, Hofer T, . Cellular background level of 8-oxo-7,8-dihydro-2′-deoxyguanosine: an isotope based method to evaluate artefactual oxidation of DNA during its extraction and subsequent work-up. Carcinogenesis 2002;23(11):1911–1918.
   PubMed Web of Science ®Google Scholar
• Kawai K, Li Y-S, Kasai H. Accurate measurement of 8-OH-dG and 8-OH-Gua in mouse DNA, urine and serum: Effects of X-ray irradiation. Genes and Environment 2007;29(3): 107–114.
   Google Scholar
• ESCODD. Inter-laboratory Validation of procedures for measuring 8-oxo-7,8-dihydroguanine/8-oxo-7,8-dihydrodeoxyguanosine in DNA. Free Radic Res 2002;36(3):239–245.
   PubMed Web of Science ®Google Scholar
• ESCOOD. Comparative analysis of baseline 8-oxo-7,8-dihydroguanine in mammalian cell DNA by different methods in different laboratories: an approach to consensus. Carcinogenesis 2002;23(12):2129–2133.
   PubMed Web of Science ®Google Scholar
• Riis B, ESCODD. Comparison of results from different laboratories in measuring 8-oxo-2′-deoxyguanosine in synthetic oligonucleotides. Free Rad Res 2002;36(6): 649–659.
   PubMed Web of Science ®Google Scholar
• ESCODD. Measurement of DNA oxidation in human cells by chromatographic and enzymic methods. Free Radic Biol Med 2003;34(8):1089–1099.
   PubMed Web of Science ®Google Scholar
• Martinez GR, Ravanat J-L, Medeiros MHG, Cadet J, Di Mascio P. Synthesis of a naphthalene endoperoxide as a source of 18O-labeled singlet oxygen for mechanistic studies. J Am Chem Soc 2000;122(41):10212–10213.
   Web of Science ®Google Scholar
• Ravanat J-L, Di Mascio P, Martinez GR, Medeiros MH, Cadet J. Singlet oxygen induces oxidation of cellular DNA. J Biol Chem 2000;275(51):40601–40604.
   PubMed Web of Science ®Google Scholar
• Halliwell B. Why and how should we measure oxidative DNA damage in nutritional studies? How far have we come? Am J Clin Nutr 2000;72:1082–1087.
   PubMed Web of Science ®Google Scholar
• Hamilton ML, Guo Z, Fuller CD, Van Remmen H, Ward WF, Austad SN, . A reliable assessment of 8-oxo-2-deoxyguanosine levels in nuclear and mitochondrial DNA using the sodium iodide method to isolate DNA. Nucleic Acids Res 2001;29(10):2117–2126.
   PubMed Web of Science ®Google Scholar
• Lenton KJ, Therriault H, Fulop T, Payette H, Wagner JR. Glutathione and ascorbate are negatively correlated with oxidative DNA damage in human lymphocytes. Carcinogenesis 1999;20(4):607–613.
   PubMed Web of Science ®Google Scholar
• Helbock HJ, Beckman KB, Shigenaga MK, Walter PB, Woodall AA, Yeo HC, Ames BN. DNA oxidation matters: the HPLC-electrochemical detection assay of 8-oxo-deoxyguanosine and 8-oxo-guanine. Proc Natl Acad Sci USA 1998;95(1): 288–293.
   PubMed Web of Science ®Google Scholar
• Hofer T, Moller L. Optimization of the workup procedure for the analysis of 8-oxo-7,8-dihydro-2′-deoxyguanosine with electrochemical detection. Chem Res Toxicol 2002; 15(3):426–432.
   PubMed Web of Science ®Google Scholar
• Nakae D, Mizumoto Y, Kobayachi E, Noguchi O, Konishi Y. Improved genomic/nuclear DNA extraction for 8-hydroxyguanosine analysis of small amounts of rat liver tissue. Cancer Lett 1995;97:233–239.
   PubMed Web of Science ®Google Scholar
• Cadet J, Douki T, Frelon S, Pouget J-P, Sauvaigo S, Ravanat J-L. Assessment of oxidative base damage to isolated and cellular DNA by HPLC-MS/MS measurement. Free Radic Biol Med 2002;33:441–449.
   PubMed Web of Science ®Google Scholar
• Badouard C, Menezo Y, Panteix G, Ravanat J-L, Douki T, Cadet J, Favier A. Determination of new types of DNA lesions in human sperm. Zygote 2008;16(1):9–13.
   PubMed Web of Science ®Google Scholar
• Collins AR, Oscoz AA, Brunborg G, Gaivao I, Giovannelli L, Kruszewski M, . The comet assay: topical issues. Mutagenesis 2008;23(3):143–151.
   PubMed Web of Science ®Google Scholar
• Collins AR. Measurement of oxidative DNA damage using the comet assay. In: Lunec J, Griffiths HR (eds). Measuring in vivo oxidative damage: A practical course. Chichester: John Wiley & Sons, LTD, 2000. pp. 83–94.
   Google Scholar
• Pflaum M, Epe B. Measuring oxidative DNA damage by alkaline elution. In: Lunec J, Griffiths HR, (eds). Measuring in vivo oxidative damage: a practical course. Chischester: John Wiley & Sons, LTD, 2000. pp. 95–104.
   Google Scholar
• Smith CC, O′Donovan MR, Martin EA. hOGG1 recognizes oxidative damage using the comet assay with greater specificity than FPG or ENDOIII. Mutagenesis 2006;21(3): 185–190.
   PubMed Web of Science ®Google Scholar
• Epe B, Hegler J. Oxidative DNA damage: Endonuclease fingerprinting. Methods Enzymol 1994;234:122–131.
   PubMed Web of Science ®Google Scholar
• Gedik CM, Boyle SP, Wood SG, Vaughan NJ, Collins AR. Oxidative stress in humans: validation of biomarkers of DNA damage. Carcinogenesis 2002;23(9):1441–1446.
   PubMed Web of Science ®Google Scholar
• Bourdat A-G, Douki T, Frelon S, Gasparutto D, Cadet J. Tandem base lesions are generated by hydroxyl radical within isolated DNA in aerated aqueous solution. J Am Chem Soc. 2000;122:4549–4556.
   Web of Science ®Google Scholar
• Hong H, Cao H, Wang Y. Formation and genotoxicity of a guanine-cytosine intrastrand cross-link lesion in vivo. Nucleic Acids Res 2007;35(21):7118–7127.
   PubMed Web of Science ®Google Scholar
• Kaufmann A, Butcher P, Maden K, Walker S, Widmer M. Quantitative and confirmative performance of liquid chromatography coupled to high-resolution mass spectrometry compared to tandem mass spectrometry. Rapid Commun Mass Spectrom 2011;25(7):979–992.
   PubMed Web of Science ®Google Scholar
• Kanaly RA, Hanaoka T, Sugimura H, Toda H, Matsui S, Matsuda T. Development of the adductome approach to detect DNA damage in humans. Antioxid Redox Signal 2006;8(5–6):993–1001.
   PubMed Web of Science ®Google Scholar
• Teng Y, Bennett M, Evans KE, Zhuang-Jackson H, Higgs A, Reed SH, Waters R. A novel method for the genome-wide high resolution analysis of DNA damage. Nucleic Acids Res 2010;39(2):e10.
   PubMed Web of Science ®Google Scholar
• Georgakilas AG, Holt SM, Hair JM, Loftin CW. Measurement of oxidatively-induced clustered DNA lesions using a novel adaptation of single cell gel electrophoresis (comet assay). Curr Protoc Cell Biol 2011;Chapter 6:Unit 6 11.
   Google Scholar
• Kryston TB, Georgiev AB, Pissis P, Georgakilas AG. Role of oxidative stress and DNA damage in human carcinogenesis. Mutat Res 2011;711(1–2):193–201.
   PubMed Web of Science ®Google Scholar
• Martin OA, Redon CE, Nakamura AJ, Dickey JS, Georgakilas AG, Bonner WM. Systemic DNA damage related to cancer. Cancer Res 2011;71(10):3437–3441.
   PubMed Web of Science ®Google Scholar
• Millau JF, Raffin AL, Caillat S, Claudet C, Arras G, Ugolin N, . A microarray to measure repair of damaged plasmids by cell lysates. Lab Chip 2008;8(10):1713–1722.
   PubMed Web of Science ®Google Scholar