257
Views
6
CrossRef citations to date
0
Altmetric
Research Article

Detection and identification of oxidants formed during NO/O2•– reaction: A multi-well plate CW-EPR spectroscopy combined with HPLC analyses

, , , &
Pages 478-486 | Received 13 Dec 2013, Accepted 20 Jan 2014, Published online: 26 Jan 2014

References

  • Kalyanaraman B, Darley-Usmar V, Davies KJ, Dennery PA, Forman HJ, Grisham MB, et al. Measuring reactive oxygen and nitrogen species with fluorescent probes: challenges and limitations. Free Radic Biol Med 2012;52:1–6.
  • Kalyanaraman B. Oxidative chemistry of fluorescent dyes: implications in the detection of reactive oxygen and nitrogen species. Biochem Soc Trans 2011;39:1221–1225.
  • Zielonka J, Zielonka M, Sikora A, Adamus J, Hardy M, Ouari O, et al. Global profiling of reactive oxygen and nitrogen species in biological systems: High-throughput real-time analyses. J Biol Chem 2012;287:2984–2995.
  • Dikalov S, Griendling KK, Harrison DG. Measurement of reactive oxygen species in cardiovascular studies. Hypertension 2007;49:717–727.
  • Zhao H, Kalivendi S, Zhang H, Joseph J, Nithipatikom K, Vasquez-Vivar J, Kalyanaraman B. Superoxide reacts with hydroethidine but forms a fluorescent product that is distinctly different from ethidium: potential implications in intracellular fluorescence detection of superoxide. Free Radic Biol Med 2003;34:1359–1368.
  • Zielonka J, Sarna T, Roberts JE, Wishart JF, Kalyanaraman B. Pulse radiolysis and steady-state analyses of the reaction between hydroethidine and superoxide and other oxidants. Arch Biochem Biophys 2006;456:39–47.
  • Back P, Back P, Matthijssens F, Vanfleteren JR, Braeckman BP. A simplified hydroethidine method for fast and accurate detection of superoxide production in isolated mitochondria. Anal Biochem 2012;423:147–151.
  • Kanamori A, Catrinescu MM, Kanamori N, Mears KA, Beaubien R, Levin LA. Superoxide is an associated signal for apoptosis in axonal injury. Brain 2010;133:2612–2625.
  • Beckman JS, Beckman TW, Chen J, Marshall PA, Freeman BA. Apparent hydroxyl radical production by peroxynitrite: implications for endothelial injury from nitric oxide and superoxide. Proc Natl Acad Sci USA 1990;87: 1620–1624.
  • Janzen EG. Spin trapping. Methods Enzymol 1984;105:188–198.
  • Zhao H, Joseph J, Zhang H, Karoui H, Kalyanaraman B. Synthesis and biochemical applications of a solid cyclic nitrone spin trap: a relatively superior trap for detecting superoxide anions and glutathiyl radicals. Free Radic Biol Med 2001;31:599–606.
  • Babich H, Zuckerbraun HL, Hirsch ST, Blau L. In vitro cytotoxicity of the nitric oxide donor, S-nitros-N-acetyl-penicillamine, towards cells from human oral tissue. Pharmacol Toxicol 1999;84:218–225.
  • Goss SPA, Hogg N, Kalyanaraman B. The effect of nitric oxide release rates on the oxidation of human low-density lipoprotein. J Biol Chem 1997;272:21647–21653.
  • Zielonka J, Sikora A, Joseph J, Kalyanaraman B. Peroxynitrite is the major species formed from different flux ratios of co-generated nitric oxide and superoxide: direct reaction with boronate-based fluorescent probe. J Biol Chem 2010;285: 14210–14216.
  • Sikora A, Zielonka J, Lopez M, Dybala-Defratyka A, Joseph J, Marcinek A, Kalyanaraman B. Reaction between peroxynitrite and boronates: EPR spin-trapping, HPLC analyses, and quantum mechanical study of the free radical pathway. Chem Res Toxicol 2011;24:687–697.
  • Keszler A, Kalyanaraman B, Hogg N. Comparative investigation of superoxide trapping by cyclic nitrone spin traps: the use of singular value decomposition and multiple linear regression analysis. Free Radic Biol Med 2003;35:1149–1157. Erratum: Free Radic Biol Med 2004;36:131.
  • Stoll S, Schweiger A. EasySpin, a comprehensive software package for spectral simulation and analysis in EPR. J Magn Reson 2006;178:42–55.
  • Zielonka J, Vasquez-Vivar J, Kalyanaraman B. Detection of 2-hydroxyethidium in cellular systems: a unique marker product of superoxide and hydroethidine. Nat Protoc 2008;3: 8–21.
  • Zielonka J, Kalyanaraman B. Hydroethidine- and MitoSOX-derived red fluorescence is not a reliable indicator of intracellular superoxide formation: another inconvenient truth. Free Radic Biol Med 2010;48:983–1001.
  • Zielonka J, Vasquez-Vivar J, Kalyanaraman B. The confounding effects of light, sonication, and MnTBAP on quantitation of superoxide using hydroethidine. Free Radic Biol Med 2006;41:1050–1057.
  • Zielonka J, Srinivasan S, Hardy M, Ouari O, Lopez M, Vasquez-Vivar J, et al. Cytochrome c-mediated oxidation of hydroethidine and mito-hydroethidine in mitochondria: Identification of homo- and heterodimers. Free Radic Biol Med 2008;44:835–846.
  • Palazzolo-Ballance AM, Suquet C, Hurst JK. Pathways for intracellular generation of oxidants and tyrosine nitration by a macrophage cell line. Biochemistry 2007;46:7536–7548.
  • Reszka KJ, McCormick ML, Buettner GR, Hart CM, Britigan BE. Nitric oxide decreases the stability of DMPO spin adducts. Nitric Oxide 2006;15:133–141.
  • Medinas DB, Cerchiaro G, Trindade DF, Augusto O. The carbonate radical and related oxidants derived from bicarbonate buffer. IUBMB Life 2007;59:255–262.
  • Zhang H, Joseph J, Felix C, Kalyanaraman B. Bicarbonate enhances the hydroxylation, nitration, and peroxidation reactions catalyzed by Cu, Zn-superoxide dismutase: intermediacy of carbonate anion radical. J Biol Chem 2000;275: 14038–14045.
  • Rothe G, Valet G. Flow cytometric analysis of respiratory burst activity in phagocytes with hydroethidine and 2’,7’-dichlorofluorescin. J Leukoc Biol 1990;47:440–8.
  • Michalski R, Michalowski B, Sikora A, Zielonka J, Kalyanaraman B. On the use of fluorescence lifetime imaging and dihydroethidium to detect superoxide in intact animals and ex vivo tissues: a reassessment. Free Radic Biol Med 2013;67C: 278–284.
  • Maghzal GJ, Cergol K, Suarna C, Newington D, Kettle AJ, Payne RJ, Stocker R. In vivo detection of myeloperoxidase activity by LC/MS/MS analysis of 2-chloro-ethidium, a novel product formed from the reaction of hydroethidine with hypochlorous acid and chloramines. Free Radic Biol Med 2012;53:S24.
  • Bonini MG, Rota C, Tomasi A, Mason RP. The oxidation of 2’,7’-dichlorofluorescin to reactive oxygen species: a self-fulfilling prophesy?Free Radic Biol Med 2006;40:968–975.
  • Kundu K, Knight SF, Less S, Taylor WR, Murthy N. A significant improvement of the efficacy of radical oxidant probes by the kinetic isotope effect. Angew Chem Int Ed 2012;49:6134–6138.
  • Pacher P, Beckman JS, Liaudet L. Nitric oxide and peroxynitrite in health and disease. Physiol Rev 2007;87:315–424.
  • Szucs S, Vámosi G, Póka R, Sárváry A, Bárdos H, Balázs M, et al. Single-cell measurement of superoxide anion and hydrogen peroxide production by human neutrophils with digital imaging fluorescence microscopy. Cytometry 1998;33: 19–31.
  • Matsunaga T, Kotamraju S, Kalivendi SV, Dhanasekaran A, Joseph J, Kalyanaraman B. Ceramide-induced intracellular oxidant formation, iron signaling, and apoptosis in endothelial cells: protective role of endogenous nitric oxide. J Biol Chem 2004;279:28614–28624.
  • Zhao H, Joseph J, Fales HM, Sokoloski EA, Levine RL, Vasquez-Vivar J, Kalyanaraman B. Detection and characterization of the product of hydroethidine and intracellular superoxide by HPLC and limitations of fluorescence. Proc Natl Acad Sci USA 2005;102:5727–5732. Erratum in: Proc Natl Acad Sci USA 2005;102:9086.
  • Laurindo FR, Fernandes DC, Santos CX. Assessment of superoxide production and NADPH oxidase activity by HPLC analysis of dihydroethidium oxidation products. Methods Enzymol 2008;441:237–260.
  • Hall DJ, Han SH, Chepetan A, Inui EG, Rogers M, Dugan LL. Dynamic optical imaging of metabolic and NADPH oxidase-derived superoxide in live mouse brain using fluorescence lifetime unmixing. J Cereb Blood Flow Metab 2012;32: 23–32.
  • Michalski R, Zielonka J, Hardy M, Joseph J, Kalyanaraman B. Hydropropidine: a novel, cell-impermeant fluorogenic probe for detecting extracellular superoxide. Free Radic Biol Med 2013;54:135–147.
  • Zhang H, Joseph J, Gurney M, Becker D, Kalyanaraman B. Bicarbonate enhances peroxidase activity of Cu,Zn- superoxide dismutase. Role of carbonate anion radical and scavenging of carbonate anion radical by metalloporphyrin antioxidant enzyme mimetics. J Biol Chem 2002; 277: 1013–1020.
  • Villamena FA, Locigno EJ, Rockenbauer A, Hadad CM, Zweier JL. Theoretical and experimental studies of the spin trapping of inorganic radicals by 5,5-dimethyl-1-pyrroline N-oxide (DMPO). 2. Carbonate radical anion. J Phys Chem A 2007;111:384–391.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.