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Free Radical Research Communications Volume 19, 1993 - Issue sup1: Third International Symposium on Spin Trapping and Aminoxyl Radical Chemistry
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Original Article

Detection of Specific Base Sequences in Solution Using DNA Probes Labeled with D- and/or 15N-substituted Spin-labels

Keisuke Makino Department of Polymer Science and Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606, Japan
,
Shunji Nagahara Department of Polymer Science and Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606, Japan
,
Yuichiro Konishi Department of Polymer Science and Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606, Japan
,
Masayuki Mukae Department of Polymer Science and Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606, Japan
,
Hiroshi Ide Department of Polymer Science and Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606, Japan
&
Akira Murakami Department of Polymer Science and Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606, Japan
Pages s109-s116 | Published online: 07 Jun 2011

References

  • K. Makino. A. Murakami, S. Nagahara. Y. Nakaisuji and T. Takeuchi (1989) A study on spin-labelled oligonucleotide synthesis and its electron spin resonance behavior in solution. Free Radical Research Communications, 6. 311–316.
  • S. Nagahara. A. Murakami and K. Makino (1992) Spin-labeled oligonucleotides sites specifically labeled at the internueteonde linkage. Separation of stereo isomeric probes and EPR spectroscopics detection of hybrid formation in solution. Nucleotides Nucleosides, II. 889–901.
  • S.D. Vcnkataramu, D.E. Pearson, A.H. Beth, R.C. Perkins, C.R. Park and J.H. Park (1981) Synthesis of pcrdeuterio-N-(l-oxyl-2,2.6,6-ietrumcihyl-4-piperidinyl) nialamide, a highly sensitive spin probe. Journal of Labelled Compounds and Radiopharmaceuticals. 18. 371–383.
  • A.H. Bclh, S.D. Venkataramu. K. Balasubramanian, UR. Dalton, B.H. Robinson. D.E. Pearson, C.R. Park and J.H. Park (1981) 15N- and 2H-substituied maleimide spin labels: Improved sensitivity and resolution for biological iiPR studies. Proceedings of the National Academy of Sciences of the United States of America. 78, 967–971.
  • H. Sayo and M-Hosokawa (1984) Simultaneous detection of plicnytoin and phénobarbital in serum by spin immunoassay. Chemical Pharmaceutical Bulletin, 32, 1675–1678.
  • B.A. Bates, M.F., Johnson and B.L. Currie (1982) Stable isotope substituted spin labels. 2. An improved synthesis of pcrdculcrio-N-(l-oxyl-2,2,6,6-ieiramcthyl-4-pipcndinyl) malcimide. Journal of Labelled Compounds and Radiopharmaceuticals, 20, 33–38.
  • M.H. Caruihers (1985) Gene synthesis machines: DNA chemistry and its uses. Science, 230. 281–285.
  • B.C. Frothier (1986) Deoxynuclcosidc H-phosphonate diester intermediate in the synthesis of intcr-nuclcotide phosphate analogues. Tetrahedron Letters, 27, 5575–5578.
  • B.C. Froehler, P-C. Ng and M.D. Matteuchi (1986) Synthesis of DNA via dcoxynuclcoside H-phosphonale intermediates. Nucleic Acids Research, 14, 5399–5407.

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