Labeling and tracking of mesenchymal stromal cells with EdU

References

• Gratzner HG, Leif RC, Ingram DJ, Castro A. The use of antibody specific for bromodeoxyuridine for the immuno-fluorescent determination of DNA replication in single cells and chromosomes. Exp Cell Res 1975;95:88–94.
   PubMed Web of Science ®Google Scholar
• Gratzner HG. Monoclonal antibody to 5-bromo- and 5-iododeoxyuridine: a new reagent for detection of DNA replication. Science 1982;218:474–5.
   PubMed Web of Science ®Google Scholar
• Dean PN, Dolbeare F, Gratzner H, Rice GC, Gray JW. Cell-cycle analysis using a monoclonal antibody to BrdUrd. Cell Tissue Kinet 1984;17:427–36.
   PubMedGoogle Scholar
• Miller MW, Nowakowski RS. Use of bromodeoxyuridine-immunohistochemistry to examine the proliferation, migration and time of origin of cells in the central nervous system. Brain Res 1988;457:44–52.
   PubMed Web of Science ®Google Scholar
• Cotsarelis G, Sun TT, Lavker RM. Label-retaining cells reside in the bulge area of pilosebaceous unit: implications for follicular stem cells, hair cycle, and skin carcinogenesis. Cell 1990;61:1329–37.
   PubMed Web of Science ®Google Scholar
• Zhang J, Niu C, Ye L, Huang H, He X, Tong WG, . Identification of the haematopoietic stem cell niche and control of the niche size. Nature 2003;425:836–41.
   PubMed Web of Science ®Google Scholar
• Tumbar T, Guasch G, Greco V, Blanpain C, Lowry WE, Rendl M, . Defining the epithelial stem cell niche in skin. Science 2004;303:359–63.
   PubMed Web of Science ®Google Scholar
• Shinin V, Gayraud-Morel B, Gomes D, Tajbakhsh S. Asymmetric division and cosegregation of template DNA strands in adult muscle satellite cells. Nat Cell Biol 2006;8:677–87.
   PubMed Web of Science ®Google Scholar
• Gage FH, Coates PW, Palmer TD, Kuhn HG, Fisher LJ, Suhonen JO, . Survival and differentiation of adult neuronal progenitor cells transplanted to the adult brain. Proc Natl Acad Sci USA 1995;92:11879–83.
   PubMed Web of Science ®Google Scholar
• Kopen GC, Prockop DJ, Phinney DG. Marrow stromal cells migrate throughout forebrain and cerebellum, and they differentiate into astrocytes after injection into neonatal mouse brains. Proc Natl Acad Sci USA 1999;96:10711–6.
   PubMed Web of Science ®Google Scholar
• Ning H, Liu G, Lin G, Yang R, Lue TF, Lin CS. Fibroblast growth factor 2 promotes endothelial differentiation of adipose tissue-derived stem cells. J Sex Med 2009;6:967–79.
   PubMed Web of Science ®Google Scholar
• Salic A, Mitchison TJ. A chemical method for fast and sensitive detection of DNA synthesis in vivo. Proc Natl Acad Sci USA 2008;105:2415–20.
   PubMed Web of Science ®Google Scholar
• Chehrehasa F, Meedeniya AC, Dwyer P, Abrahamsen G, Mackay-Sim A. EdU, a new thymidine analogue for labelling proliferating cells in the nervous system. J Neurosci Methods 2009;177:122–30.
   PubMed Web of Science ®Google Scholar
• Lin G, Garcia M, Ning H, Banie L, Guo YL, Lue TF, . Defining stem and progenitor cells within adipose tissue. Stem Cells Dev 2008;17:1053–63.
   PubMed Web of Science ®Google Scholar
• Sievert KD, Emre Bakircioglu M, Tsai T, Dahms SE, Nunes L, Lue TF. The effect of simulated birth trauma and/or ovariectomy on rodent continence mechanism. I. Functional and structural change. J Urol 2001;166:311–7.
   PubMed Web of Science ®Google Scholar
• Banie L, Lin G, Ning H, Wang G, Lue TF, Lin CS. Effects of estrogen, raloxifene and levormeloxifene on alpha1A-adrenergic receptor expression. J Urol 2008;180:2241–6.
   PubMed Web of Science ®Google Scholar
• Rahman NU, Phonsombat S, Bochinski D, Carrion RE, Nunes L, Lue TF. An animal model to study lower urinary tract symptoms and erectile dysfunction: the hyperlipidaemic rat. BJU Int 2007;100:658–63.
   PubMed Web of Science ®Google Scholar
• Ning H, Lin G, Lue TF, Lin CS. Neuron-like differentiation of adipose tissue-derived stromal cells and vascular smooth muscle cells. Differentiation 2006;74:510–8.
   PubMed Web of Science ®Google Scholar
• Potten CS, Owen G, Booth D. Intestinal stem cells protect their genome by selective segregation of template DNA strands. J Cell Sci 2002;115:2381–8.
   PubMed Web of Science ®Google Scholar
• Mirza A, Hyvelin JM, Rochefort GY, Lermusiaux P, Antier D, Awede B, . Undifferentiated mesenchymal stem cells seeded on a vascular prosthesis contribute to the restoration of a physiologic vascular wall. J Vasc Surg 2008;47:1313–21.
   PubMed Web of Science ®Google Scholar
• Sauerzweig S, Baldauf K, Braun H, Reymann KG. Time-dependent segmentation of BrdU-signal leads to late detection problems in studies using BrdU as cell label or proliferation marker. J Neurosci Methods 2009;177:149–59.
   Google Scholar