Human adipose-derived mesenchymal stem cells in vitro: evaluation of an optimal expansion medium preserving stemness

References

• Gronthos S, Franklin DM, Leddy HA, Robey PG, Storms RW, Gimble JM. Surface protein characterization of human adipose-derived stromal cells. J Cell Physiol 2001;189:54–63.
   PubMed Web of Science ®Google Scholar
• De Ugarte DA, Morizono K, Elbarbary A, Alfonso Z, Zuk PA, Zhu M, . Comparison of multi-lineage cells from human adipose tissue and bone marrow. Cells Tissues Organs 2003;174:101–9.
   PubMed Web of Science ®Google Scholar
• Strem BM, Hicok KC, Zhu M, Wulur I, Alfonso Z, Schreiber RE, . Multilineage differentiation of adipose tissue-derived stem cells. Keio J Med 2005;54:132–41.
   PubMed Web of Science ®Google Scholar
• Wagner W, Wein F, Seckinger A, Frankhauser M, Wirkner U, Krause U, . Comparative characteristics of mesenchymal stem cells from human bone marrow, adipose tissue, and umbilical cord blood. Exp Hematol 2005;33:1402–16.
   PubMed Web of Science ®Google Scholar
• Kern S, Eichler H, Stoeve J, Klüter H, Bieback K. Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord or adipose tissue. Stem Cells 2006;24:1294–301.
   PubMed Web of Science ®Google Scholar
• Zuk PA, Zhu M, Mizuno H, Huang JI, Futrell JW, Katz AJ, . Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng. 2001;7:211–28.
   PubMedGoogle Scholar
• Gimble JM, Guilak F. Differential potential of adipose derived adult stem (ADAS) cells. Curr Topics Dev Biol 2003;58:137–60.
   PubMed Web of Science ®Google Scholar
• Ho AD, Wagner W, Franke W. Heterogeneity of mesenchymal stromal cell preparations. Cytotherapy 2008;10:320–30.
   PubMed Web of Science ®Google Scholar
• Brzoska M, Gauer S, Geiger H, Baer PC. Epithelial differentiation of human adipose tissue-derived adult stem cells. Biochem Biophys Res Commun 2005;330:142–50.
   PubMed Web of Science ®Google Scholar
• Baer PC, Bereiter-Hahn J, Missler C, Brzoska M, Schubert R, Gauer S, . Conditioned medium from renal tubular epithelial cells initiates differentiation of human mesenchymal stem cells. Cell Proliferation 2009:42:29–37.
   PubMed Web of Science ®Google Scholar
• Gimble JM, Katz AJ, Bunnell BA. Adipose-derived stem cells for regenerative medicine. Circ Res 2007;100:1249–60.
   PubMed Web of Science ®Google Scholar
• Riordan NH, Ichim TE, Min WP, Wang H, Solano F, Lara F, . Non-expanded adipose stromal vascular fraction cell therapy for multiple sclerosis. J Transl Med 2009;7:29.
   PubMed Web of Science ®Google Scholar
• Suga H, Shigeura T, Matsumoto D, Inoue K, Kato H, Aoi N, . Rapid expansion of human adipose-derived stromal cells preserving multipotency. Cytotherapy 2007;9:738–45.
   PubMed Web of Science ®Google Scholar
• Zuk PA, Zhu M, Ashjian P, De Ugarte DA, Huang JI, Mizuno H, . Human adipose tissue is a source of multipotent stem cells. Molecul Biol Cell 2002;13:4279–95.
   PubMed Web of Science ®Google Scholar
• Baer PC, Schubert R, Bereiter-Hahn J, Plösser M, Geiger H. Expression of a functional epidermal growth factor receptor on human adipose-derived mesenchymal stem cells and its signaling mechanism. Eur J Cell Biol 2009;88:273–83.
   PubMed Web of Science ®Google Scholar
• Pfaffl MW. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 2001; 29:e45.
   PubMed Web of Science ®Google Scholar
• Kilroy GE, Foster SJ, Wu X, Ruiz J, Sherwood S, Heifetz A, . Cytokine profile of human adipose-derived stem cells: expression of angiogenic, hematopoietic, and pro-inflammatory factors. J Cell Physiol 2007;212:702–29.
   PubMed Web of Science ®Google Scholar
• Van Harmelen V, Röhrig K, Hauner H. Comparison of proliferation and differentiation capacity of human adipocyte precursor cells from the omental and subcutaneous adipose tissue depot of obese subjects. Metabolism 2004;53:632–7.
   PubMed Web of Science ®Google Scholar
• Oedayrajsingh-Varma MJ, van Ham SM, Knippenberg M, Helder MN, Klein-Nulend J, Schouten TE, . Adipose tissue-derived mesenchymal stem cell yield and growth characteristics are affected by the tissue-harvesting procedure. Cytotherapy 2006;8:166–77.
   PubMed Web of Science ®Google Scholar
• Fraser JK, Wulur I, Alfonso Z, Zhu M, Wheeler ES. Differences in stem and progenitor cell yield in different subcutaneous adipose tissue depots. Cytotherapy 2007:9:459–67.
   PubMed Web of Science ®Google Scholar
• Varma MJ, Breuls RG, Schouten TE, Jurgens WJ, Bontkes HJ, Schuurhuis GJ, . Phenotypical and functional characterization of freshly isolated adipose tissue-derived stem cells. Stem Cells Dev 2007;16:91–104.
   PubMed Web of Science ®Google Scholar
• Jurgens WJ, Oedayrajsingh-Varma MJ, Helder MN, Zandiehdoulabi B, Schouten TE, Kuik DJ, . Effect of tissue-harvesting site on yield of stem cells derived from adipose tissue: implications for cell-based therapies. Folia Histochem Cytobiol 2008;46:307–14.
   PubMedGoogle Scholar
• Padoin AV, Braga-Silva J, Martins P, Rezende K, Rezende AR, Grechi B, . Sources of processed lipoaspirate cells: influence of donor site on cell concentration. Plast Reconstr Surg 2008;122:614–8.
   PubMedGoogle Scholar
• Dominici M, Paolucci P, Conte P, Horwitz EM. Heterogeneity of multipotent mesenchymal stromal cells: from stromal cells to stem cells and vice versa. Transplant. 2009;87:S36–42.
   Web of Science ®Google Scholar
• Horwitz EM, Dominici M. How do mesenchymal stromal cells exert their therapeutic benefit? Cytotherapy 2008;10:771–4.
   PubMed Web of Science ®Google Scholar
• Wagner W, Ho AD. Mesenchymal stem cell preparations: comparing apples and oranges. Stem Cell Rev 2007;3:239–48.
   PubMed Web of Science ®Google Scholar
• Sotiropoulou PA, Perez SA, Salagianni M, Baxevanis CN, Papamichail M. Characterization of the optimal culture conditions for clinical scale production of human mesenchymal stem cells. Stem Cells 2006;24:462–71.
   PubMed Web of Science ®Google Scholar
• Perez-Ilzarbe M, Diez-Campelo M, Aranda P, Tabera S, Lopez T, del Canizo C, . Comparison of ex vivo expansion culture conditions of mesenchymal stem cells for human cell therapy. Transfusion 2009;49:1901–10.
   PubMed Web of Science ®Google Scholar
• Reyes M, Lund T, Lenvik T, Aguiar D, Koodie L, Verfailles CM. Purification and ex vivo expansion of postnatal human marrow mesodermal progenitor cells. Blood 2001;98:2615–25.
   PubMed Web of Science ®Google Scholar
• Pochambally RR, Smith JR, Ylostalo J, Prockop DJ. Serum deprivation of human marrow stromal cells (hMSCs) selects for a subpopulation of early progenitor cells with enhanced expression of OCT-4 and other embryonic genes. Blood 2004;103:1647–52.
   PubMed Web of Science ®Google Scholar
• Bernardo ME, Avanzini MA, Perotti C, Cometa AM, Moretta A, Lenta E, . Optimization of in vitro expansion of human multipotent mesenchymal stromal cells for cell-therapy approaches: further insights in the search for a fetal calf serum substitute. J Cell Physiol 2007;211:121–30.
   PubMed Web of Science ®Google Scholar
• Doucet C, Ernou I, Zhang Y, Llense JR, Begot L, Holy X, . Platelet lysates promote mesenchymal stem cell expansion: a safety substitute for animal serum in cell-based therapy applications. J Cell Physiol 2005;205:228–36.
   PubMed Web of Science ®Google Scholar
• Muller I, Kordowich S, Holzwarth C, Spano C, Isensee G, Staiber A, . Animal serum-free culture conditions for isolation and expansion of multipotent mesenchymal stromal cells from human BM. Cytotherapy 2006;8:437–44.
   PubMed Web of Science ®Google Scholar
• Lund P, Pilgaard L, Duroux M, Fink T, Zachar V. Effect of growth media and serum replacements on the proliferation and differentiation of adipose-derived stem cells. Cytotherapy 2009;11:189–97.
   PubMed Web of Science ®Google Scholar
• Lin TM, Tsai JL, Lin SD, Lai CS, Chang CC. Accelerated growth and prolonged lifespan of adipose tissue-derived human mesenchymal stem cells in a medium using reduced calcium and antioxidants. Stem Cells Dev 2005;14:92–102.
   PubMed Web of Science ®Google Scholar
• Iwashima S, Ozaki T, Maruyama S, Saka Y, Kobori M, Omae K, . Novel culture system of mesenchymal stromal cells from human subcutaneous adipose tissue. Stem Cells Dev 2009;18:533–43.
   PubMed Web of Science ®Google Scholar
• Lee SY, Lim J, Khang G, Youngsook S, Choung PH, Kang SS, . Enhanced ex vivo expansion of human adipose tissue-derived mesenchymal stromal cells by fibroblast growth factor-2 and dexamethasone. Tissue Eng Part A 2009;15:2491–9.
   PubMed Web of Science ®Google Scholar
• Chun-Mao H, Su-Yi W, Ping-Ping L, Hang-Hui C. Human bone marrow-derived mesenchymal stem cells differentiate into epidermal-like cells in vitro. Differentiation 2007;75:292–8.
   PubMed Web of Science ®Google Scholar
• Apel A, Groth A, Bruns H, Schemmer P, Büchler MW, Herr I. Suitability of human mesenchymal stem cells for gene therapy depends on the expansion medium. Exp Cell Res 2009;315:498–507.
   PubMed Web of Science ®Google Scholar
• Jin HJ, Park SK, Oh W, Yang YS, Kim SW. Down-regulation of CD105 is associated with multi-lineage differentiation in human umbilical cord blood-derived mesenchymal stem cells. Biochem Biophys Res Comm 2009;381:676–81.
   PubMed Web of Science ®Google Scholar
• Mitchell JB, McIntosh K, Zvonic S, Garretta S, Floyd ZE, Kloster A, . Immunophenotype of human adipose-derived cells: temporal changes in stromal-associated and stem cell-associated markers. Stem Cells 2006;24:376–85.
   PubMed Web of Science ®Google Scholar