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Research Article

Partial purification and characterization of lipase from Geobacillus stearothermophilus AH22

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Pages 325-331 | Received 27 Jan 2015, Accepted 22 Feb 2015, Published online: 23 Mar 2015

References

  • Svendsen A. Review: lipase protein engineering. Biochem Biophys Acta 2000;1543:223–38
  • Verma ML, Kanwar SS. Properties and application of poly hydrogel immobilized Bacillus cereus MTCC 8372 lipase for the synthesis of geranylacetate. J Appl Polymer Sci 2008;110:837–46
  • Marek A, Bednarski W. Some factors affecting lipase production by yeasts and filamentous fungi. Biotechnol Lett 1996;18:1155–60
  • Gupta R, Gupta N, Rathi P. Bacterial lipases: an overview of production, purification and biochemical properties. Appl Microbiol Biotechnol 2004;64:763–81
  • Singh AK, Mukhopadhyay M. Overview of fungal lipase: a review. Appl Biochem Biotechnol 2012;166:486–520
  • Alberghina L, Schmid RD, Verger R. eds. Lipases: structure, mechanism and genetic engineering. Weinheim: Wiley-VCH; 1991
  • Bornscheuer UT. Enzymes in lipid modification. Weinheim: Wiley-VCH, Greifswald University; 2000
  • Adiguzel A, Ozkan H, Baris O, et al. Identification and characterization of thermophilic bacteria isolated from hot springs in Turkey. J Microbiol Methods 2009;79:321–8
  • Winkler UK, Stuckman M. Glycogen hyaluronate and some other polysaccharides greatly enhance the formation of exolipase by Serratia marcescens. J Bacteriol 1979;138:663–70
  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951;193:265–75
  • Laemmli UK. Cleavage of structural proteins during the assembly of the head. Nature 1970;227:680–5
  • Mastropaolo W, Yourno J. An ultraviolet spectrophotometric assay for 1 – naphtylacetate and naphtylbutyrate esterases. Anal Biochem 1981;115:188–93
  • Kambourova M, Kirilova N, Mandeva R, Derekova A. Purification and properties of thermostable lipase from a thermophilic Bacillus stearothermophilus MC 7. J Mol Catal B Enzym 2003;22:307–13
  • Masomian M, Abd Rahman RNZR, Salleh AB, Basri M. A new thermostable and organic solvent-tolerant lipase from Aneurinibacillus thermoaerophilus strain HZ. Process Biochem 2013;48:169–75
  • Lesuisse E, Schanck K, Colson C. Purification and preliminary characterization of the extracellular lipase of Bacillus subtilis 168, an extremely basic pH-tolerant enzyme. Eur J Biochem 1993;216:155–60
  • Saxena RK, Davidson WS, Sheoran A, Giri B. Purification and characterization of an alkaline thermostable lipase from Aspergillus carneus. Process Biochem 2003;39:239–47
  • Zheng YY, Guo XH, Song NN, Li DC. Thermophilic lipase from Thermomyces lanuginosus: gene cloning, expression and characterization. J Mol Catal B Enzym 2011;69:127–32
  • Cao Y, Zhuang Y, Yao C, et al. Purification and characterization of an organic solvent-stable lipase from Pseudomonas stutzeri LC2-8 and its application for efficient resolution of (R,S)-1-phenylethanol. Biochem Eng J 2012;64:55–60
  • Lee DW, Kim HW, Lee KW, et al. Purification and characterization of two distinct thermostable lipases from the gram-positive thermophilic bacterium Bacillus thermoleovorans ID-1. Enzyme Microb Technol 2001;29:363–71
  • Chen S, Qian L, Shi B. Purification and properties of enantio selective lipase from a newly isolated Bacillus cereus C71. Process Biochem 2007;42:988–94
  • Joshi C, Khare SK. Purification and characterization of Pseudomonas aeruginosa lipase produced by SSF of deoiled Jatropha seed cake. Biocatal Agric Biotechnol 2013;2:32–7
  • Li XL, Zhang WH, Wang YD, et al. A high-detergent-performance, cold-adapted lipase from Pseudomonas stutzeri PS59 suitable for detergent formulation. J Mol Catal B Enzym 2014;102:16–24
  • Dheeman SD, Antony-Babu S, Frías JM, Henehan GTM. Purification and characterization of an extracellular lipase from a novel strain Penicillium sp. DS-39 (DSM 23773). J Mol Catal B Enzym 2009;72:256–62
  • Joseph B, Shrivastava N, Ramteke PW. Extracellular cold-active lipase of Microbacterium luteolum isolated from Gangotri glacier, western Himalaya: isolation, partial purification and characterization. J Genet Eng Biotechnol 2012;10:137–44
  • Jermsuntiea W, Aki T, Toyoura R, et al. Purification and characterization of intracellular lipase from the polyunsaturated fatty acid-producing fungus Mortierella alliacea. New Biotechnol 2011;28:158–64
  • Sarkar P, Yamasaki S, Basak S, et al. Purification and characterization of a new alkali-thermostable lipase from Staphylococcus aureus isolated from Arachis hypogaea rhizosphere. Process Biochem 2012;47:858–66
  • Sinchaikul S, Sookkheo B Phutrakul S, et al. Optimization of a thermostable lipase from Bacillus stearothermophilus P1: overexpression, purification, and characterization. Protein Express Purif 2001;22:388–98
  • Nawani N, Khurana J, Kaur J. A thermostable lipolytic enzyme from a thermophilic Bacillus sp.: purification and characterization. Mol Cell Biochem 2006;290:17–22
  • Liu Z, Chi Z, Wang L, Li J. Production, purification and characterization of an extracellular lipase from Aureobasidium pullulans HN2.3 with potential application for the hydrolysis of edible oils. Biochem Eng J 2008;40:445–51
  • Tayyab M, Rashid N, Akhtar M. Isolation and identification of lipase producing thermophilic Geobacillus sp. SBS-4S: cloning and characterization of the lipase. J Biosci Bioeng 2009;111:272–8
  • Fullbrook PD. Practical applied kinetics. In: Godfrey T, West S, eds. Industrial enzymology. London: Macmillan; 1996:483–501
  • Florczak T, Daroch M, Wilkinson MC, et al. Purification, characterisation and expression in Saccharomyces cerevisiae of LipG7 an enantioselective, cold-adapted lipase from the Antarctic filamentous fungus Geomyces sp. P7 with unusual thermostability characteristics. Enzym Microb Technol 2013;53:18–24
  • Barth G, Gaillardin C. Physiology and genetics of the dimorphic fungus Yarrowia lipolytica. FEMS Microbiol Rev 1997;19:219–37
  • Gaur R, Gupta A, Khare SK. Purification and characterization of lipase from solvent tolerant Pseudomonas aeruginosa PseA. Process Biochem 2008;43:1040–6
  • Ji Q, Xiao S, He B, Liu X. Purification and characterization of an organic solvent-tolerant lipase from Pseudomonas aeruginosa LX1 and its application for biodiesel production. J Mol Catal B Enzym 2010;66:264–9
  • Macrae AR, Hammond RC. Present and future applications of lipases. Biotechnol Genet Eng Rev 1985;3:193–217
  • Godtfredsen SE. Microbial lipases. In: Fogarty WM, Kelly ET, Eds. Microbial enzymes and biotechnology. Amsterdam: Elsevier; 1990:255–73
  • Ramirez-Zavala B, Mercado-Flores Y, Hernadez-Rodriguez C, Villa-Tanaca L. Purification and characterization of lysine aminopeptidase from Kluyveromyces marxiamus. FEMS Microbiol Lett 2004;235:369–75
  • Chartrain M, Katz L, Marcin C, et al. Purification and characterization of a novel bioconverting lipase from Pseudomonas aeruginosa MB 5001. Enzyme Microb Technol 1993;15:575–80
  • Gao XG, Cao SG, Zhang KC. Production, properties and application to nonaqueous enzymatic catalysis of lipase from a newly isolated Pseudomonas strain. Enzyme Microb Technol 2000;27:74–82
  • Ramani K, Kennedy LJ, Ramakrishnan M, Sekarana G. Purification, characterization and application of acidic lipase from Pseudomonas gessardii using beef tallow as a substrate for fats and oil hydrolysis. Process Biochem 2010;45:1683–91
  • Tamilarasan K, Kumar MD. Purification and characterization of solvent tolerant lipase from Bacillus sphaericus MTCC 7542. Biocatal Agric Biotechnol 2012;1:309–13
  • Dandavate V, Jinjala J, Keharia H, Madamwar D. Production, partial purification and characterization of organic solvent tolerant lipase from Burkholderia multivorans V2 and its application for ester synthesis. Bioresource Technol 2009;100:3374–81
  • Supakdamrongkul P, Bhumiratana A, Wiwat C. Characterization of an extracellular lipase from the biocontrol fungus, Nomuraea rileyi MJ, and its toxicity toward Spodoptera litura. J Invertebr Pathol 2010;105:228–35

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