69
Views
2
CrossRef citations to date
0
Altmetric
Research Article

A real-time PCR assay for differential expression of vitellogenin I and II genes in the liver of the sentinel fish species Lipophrys pholis

, , , , &
Pages 591-597 | Received 22 Feb 2013, Accepted 26 May 2013, Published online: 08 Oct 2013

References

  • Ankley GT, Johnson RD. (2004). Small fish models for identifying and assessing the effects of endocrine-disrupting chemicals. ILAR J 45:469–83
  • Ashby J, Houthoff E, Kennedy SJ, et al. (1997). The challenge posed by endocrine-disrupting chemicals. Environ Health Perspect 105:164–9
  • Barucca M, Canapa A, Olmo E, Regoli F. (2006). Analysis of vitellogenin gene induction as a valuable biomarker of estrogenic exposure in various Mediterranean fish sp. Environ Res 101:68–73
  • Beeby A. (2001). What do sentinels stand for? Environ Pollut 112:285–98
  • Brown M, Robinson C, Davies IM, et al. (2004). Temporal changes in gene expression in the liver of male plaice (Pleuronectes platessa) in response to exposure to ethynyloestradiol analysed by macroarray and real-time PCR. Mutat Res 552:35–49
  • Crews D, Willingham E, Skipper JK. (2000). Endocrine disruptors: present issues, future directions. Q Rev Biol 75:243–60
  • Faria C, Almada VC, Goncalves EJ. (1996). Juvenile recruitment, growth and maturation of Lipophrys pholis (Pisces: Blenniidae), from the west coast of Portugal. J Fish Biol 49:727–30
  • Ferreira F, Santos MM, Castro LFC, et al. (2009). Vitellogenin gene expression in the intertidal blenny Lipophrys pholis: a new sentinel species for estrogenic chemical pollution monitoring in the European Atlantic coast? Comp Biochem Phys C 149:58–64
  • Ferreira F, Santos MM, Reis-Henriques MA, et al. (2010). Sexing blennies using genital papilla morphology or ano-genital distance. J Fish Biol 77:1432–8
  • Ferreira F, Santos MM, Reis-Henriques MA, et al. (2011). The annual cycle of spermatogenesis in Lipophrys pholis (Blenniidae), a recently proposed sentinel species for pollution monitoring. Ichthyol Res 58:360–5
  • Ferreira F, Santos MM, Reis-Henriques MA, et al. (2012). The annual cycle of oogenesis in the Shanny, Lipophrys pholis. Scien Mar 76:273–80
  • García-Reyero N, Raldúa D, Quirós L, et al. (2004). Use of vitellogenin mRNA as a biomarker for endocrine disruption in feral and cultured fish. Anal Bioanal Chem 378:670–5
  • Gonçalves AF, Castro LF, Pereira-Wilson C, et al. (2007). Is there a compromise between nutrient uptake and gas exchange in the gut of Misgurnus anguillicaudatus, an intestinal air-breathing fish? Comp Biochem Physiol Part D 4:345–55
  • Hagger JA, Jones MB, Lowe D, et al. (2008). Application of biomarkers for improving risk assessments of chemicals under the Water Framework Directive: a case study. Marine Poll Bull 56:1111–18
  • Harvey JS, Lyons BP, Page TS, et al. (1999). An assessment of the genotoxic impact of the Sea Empress oil spill by the measurement of DNA adduct levels in selected invertebrate and vertebrate species. Mutat Res Gen Tox En 441:103–14
  • Hemmer MJ, Bowman CJ, Hemmer BL, et al. (2002). Vitellogenin mRNA regulation and plasma clearance in male sheepshead minnows, (Cyprinodon variegatus) after cessation of exposure to 17β-estradiol and p-nonylphenol. Aquat Toxicol 58:99–112
  • Henry TB, McPherson JT, Rogers ED, et al. (2009). Changes in the relative expression pattern of multiple vitellogenin genes in adult male and larval zebrafish exposed to exogenous estrogens. Comp Biochem Phys A 154:119–26
  • Hiramatsu N, Matsubara T, Fujita T, et al. (2006). Multiple piscine vitellogenins: biomarkers of fish exposure to estrogenic endocrine disruptors in aquatic environments. Mar Biol 149:35–47
  • Islinger M, Willimski D, Volkl A, Braunbeck T. (2003). Effects of 17α-ethinylestradiol on the expression of three estrogen-responsive genes and cellular ultrastructure of liver and testes in male zebrafish. Aquat Toxicol 62:85–103
  • Jobling S, Nolan M, Tyler CR, et al. (1998). Widespread sexual disruption in wild fish. Environ Sci Technol 32:2498–506
  • Jobling S, Casey D, Rodgers-Gray T, et al. (2004). Comparative responses of molluscs and fish to environmental estrogens and an estrogenic effluent. Aquat Toxicol 66:207–22
  • Kime DE, Nash JP, Scott AP. (1999). Vitellogenesis as a biomarker of reproductive disruption by xenobiotics. Aquaculture 177:345–52
  • Kirby MF, Bignell J, Brown E, et al. (2003). The presence of morphologically intermediate papilla syndrome in United Kingdom populations of sand goby (Pomatochistus spp.): endocrine disruption? Environ Toxicol Chem 22:239–51
  • Knoebl I, Hemmer MJ, Denslow ND. (2004). Induction of zona radiate and vitellogenin genes in estradiol and nonylphenol exposed male sheepshead minnows (Cyprinodon variegates). Mar Environ Res 58:547–51
  • Lima D, Santos MM, Ferreira AM, et al. (2008). The use of the shanny Lipophrys pholis for pollution monitoring: a new sentinel species for the northwestern European marine ecosystems. Environ Int 34:94–101
  • Livak KJ, Schmittgen TD. (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods 25:402–8
  • Lower WR, Kendall RJ. (1990). Sentinel species and sentinel bioassay. In: McCarthy JF, Shugart LR, eds. Biomarkers of environmental contamination. Boca Raton (FL): Lewis Publishers, 309–31
  • Lyons BP, Harvey JS, Parry JM. (1997). An initial assessment of the genotoxic impact of the Sea Empress oil spill by the measurement of DNA adduct levels in the intertidal teleost Lipophrys pholis. Mutat Res Gen Tox En 390:263–8
  • Matsubara T, Nagae M, Ohkubo N, et al. (2003). Multiple vitellogenins and their unique roles in marine teleosts. Fish Physiol Biochem 28:295–9
  • Miracle A, Ankley G, Lattier D. (2006). Expression of two vitellogenin genes (vg1 and vg3) in fathead minnow (Pimephales promelas) liver in response to exposure to steroidal estrogens and androgens. Ecotoxicol Environ Safety 63:337–42
  • Monteiro NM, Quinteira SM, Silva K, et al. (2005). Diet preference reflects the ontogenic shift in microhabitat use in Lipophrys pholis. J Fish Biol 67:102–13
  • OECD (1999). Final report of the fish expert consultation meeting, London, U.K. 28–29 October. Paris, France: Organization for Economic Co-operation and Development, Environmental Health and Safety Division
  • OECD (2000). Report of the OECD expert consultation on testing in fish-EDF2, Tokyo, March 2000. Paris, France: Organization for Economic Co-operation and Development
  • OECD (2004). OECD draft report of the initial work towards the validation of the fish screening assay for the detection of endocrine active substances: phase 1A. Paris, France: Organization for Economic Co-operation and Development
  • Rodrigues P, Reis-Henriques MA, Campos J, Santos MM. (2006). Urogenital Papilla feminization in male Pomatoschistus minutus from two estuaries in northwestern Iberian Peninsula. Mar Environ Res 62:S258–62
  • Sanchez W, Porcher JM. (2009). Fish biomarkers for environmental monitoring within the water Framework Directive of the European Union. Trends Anal Chem 28:150–8
  • Santos MM, Solé M, Lima D, et al. (2010). Validating a multi-biomarker approach with the shanny Lipophrys pholis to monitor oil spills in European marine ecosystem. Chemosphere 81:685–91
  • Scholz S, Kordes C, Haman J, Gutzeit HO. (2004). Induction of vitellogenin in vivo and in vitro in the model teleost medaka (Oryzias latipes): comparison of gene expression and protein levels. Mar Environ Res 57:235–44
  • Soares J, Coimbra AM, Reis-Henriques MA, et al. (2009). Disruption of zebrafish (Danio rerio) embryonic development after full life-cycle parental exposure to low levels of ethinylestradiol. Aquat Toxicol 95:330–8
  • Solé M, Raldua D, Piferrer F, et al. (2003). Feminization of wild carp, Cyprinus carpio, in a polluted environment: plasma steroid hormones, gonadal morphology and xenobiotic metabolizing system. Comp Biochem Phys C 136:145–56
  • Solé M, Lobera G, Lima D, et al. (2008). Esterases activities and lipid peroxidation levels in muscle tissue of the shanny Lipophrys pholis along several sites from the Portuguese Coast. Mar Pollut Bull 56:999–1007
  • Sumpter JP, Jobling S. (1995). Vitellogenesis as a biomarker for estrogenic contamination of the aquatic environment. Environ Health Perspect 103:173–8
  • Sumpter JP. (2005). Endocrine disruptors in the aquatic environment: an overview. Acta Hydroch Hydrob 33:9–16
  • Tamura K, Dudley J, Nei M, Kumar S. (2007). MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596--9
  • Thompson JD, Gibson TJ, Plewniak F, et al. (1997). The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–82
  • Trichet V, Buisine N, Mouchel N, et al. (2000). Genomic analysis of the vitellogenin locus in rainbow trout (Oncorhynchus mykiss) reveals a complex history of gene amplification and retroposon activity. Mol Gen Genet S263:828–37
  • Van Aerle R, Nolan TM, Jobling S, et al. (2001). Sexual disruption in a second species of wild cyprinid fish (the gudgeon, Gobio gobio) in United Kingdom freshwaters. Environ Toxicol Chem 20:2841–7
  • Wang H, Tan JTT, Emelyanov A, et al. (2005). Hepatic and estrahepatic expression of vitellogenin genes in the zebrafish Danio rerio. Gene 356:91–100
  • Yamaguchi A, Ishibashi H, Kohra S, et al. (2009). Expression analysis of estrogen-responsive genes vitellogenin 1 and 2 in liver of male medaka (Oryzias latipes) exposed to selective ligands of estrogen receptor subtypes. J Health Sci 55:930–8

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.