169
Views
9
CrossRef citations to date
0
Altmetric
Original Article

Contortrostatin, a Homodimeric Disintegrin, Actively Disrupts Focal Adhesion and Cytoskeletal Structure and Inhibits Cell Motility Through a Novel Mechanism

, &
Pages 71-86 | Published online: 11 Jul 2009

References

  • Angers-Loustau A., Cote J.-F., Charest A., Dowbenko D., Spencer S., Lasky L. A., Tremblay M. L. Protein tyrosine phosphatase-PEST regulates Focal adhesion disassembly, migration, and cytokinesis in fibroblasts. J. Cell Biol. 1999; 144: 1019–1031
  • Aznavoorian S., Stracke M. L., Parsons J., McClanahan J., Liotta L. A. Integrin alphavbeta3 mediates chemotactic and haptotactic motility in human melanoma cells through different signaling pathways. J. Biol. Chem. 1996; 271: 3247–3254
  • Beviglia L., Stewart G. J., Niewiarowski S. Effect of four disintegrins on the adhesive and metastatic properties of B16F10 melanoma cells in a murine model. Oncology Research 1995; 7: 7–20
  • Bhattacharya S., Fu C., Bhattacharya J., Greenberg S. Soluble ligands of the αvβ3 integrin mediate enhanced tyrosine phosphorylation of multiple proteins in adherent bovine pulmonary artery endothelial cells. J. Biol. Chem. 1995; 270: 16781–16787
  • Brooks P. C., Clark R. A., Cheresh D. A. Requirement of vascular integrin αvβ3 for angiogenesis. Science 1994a; 264: 569–571
  • Brooks P. C., Montgomery A. M.P., Rosenfeld M., Reisfeld R. A., Hu T., Klier G., Cheresh D. A. Integrin αvβ3 antagonists promote tumor regression by inducing apoptosis of angiogenic blood vessels. Cell 1994b; 79: 1157–1164
  • Brooks P. C., Silletti S., Von Schalscha T. L., Friedlander M., Cheresh D. A. Disruption of angiogenesis by PEX, a noncatalytic metalloproteinase fragment with integrin binding activity. Cell 1998; 92: 391–400
  • Brooks P. C., Stromblad S., Sanders L. C., Von Schalscha T. L., Aimes R. T., Stetler-Stevenson W. G., Quigley J. P., Cheresh D. A. Localization of matrix metalloproteinase MMP-2 to the surface of invasive cells by interaction with integrin αvβ3. Cell 1996; 85: 683–693
  • Burridge K., Fath K., Kelly T., Nuckolls G., Turner C. Focal adhesions: transmembrane junctions between the extracellular matrix and the cytoskeleton. A. Rev. Cell Biol. 1988; 4: 487–525
  • Burridge K., Turner C. E., Romer L. H. Tyrosine phosphorylation of paxillin and pp125FAKaccompanies cell adhesion to extracellular matrix: A role in cytoskeletal assembly. J. Cell Biol. 1992; 119: 893–903
  • Cary L. A., Han D. C., Polte T. R., Hanks S. K., Guan J.-L. Identification of p130Casas a mediator of focal adhesion kinase-promoted cell migration. J. Cell Biol. 1998; 140: 211–221
  • Cobb B. S., Schaller M. D., Leu T. H., Parsons J. T. Stable association of pp60src and pp59fyn with the focal adhesion-associated protein tyrosine kinase, pp125 FAK. Mol. Cell. Biol. 1994; 14: 147–155
  • Friedman E., Verderame M., Lipkin M., Pollack R. Altered actin cytoskeletal patterns in two premalignant stages in human colon carcinoma development. Cancer Research 1985; 45: 3236–3242
  • Garton A. J., Tonks N. K. Regulation of fibroblast motility by the protein tyrosine phosphatase PTP-PEST. J. Biol. Chem. 1999; 274: 3811–3818
  • Giancotti F. G., Ruoslahti E. Integrin signaling. Science 1999; 285: 1028–1032
  • Gilmore A. P., Romer L. H. Inhibition of focal adhesion kinase (FAK) signaling in focal adhesions decreases cell motility and proliferation. Mol. Biol. Cell 1996; 7: 1209–1224
  • Huang T.-F., Holt J. C., Lukasiewicz H., Niewiarowski S. Trigramin: A low molecular weight peptide inhibiting fibrinogen interaction with platelet expressed on glycoprotein IIb-IIIa complex. J. Biol. Chem. 1987; 262: 16157–16163
  • Kang I.-C., Lee Y.-D., Kim D.-S. A novel disin-tegrin salmosin inhibits tumor angiogenesis. Cancer Research 1999; 59: 3754–3760
  • Keely P., Parise L., Juliano R. Integrins and GTPases in tumour cell growth, motility and invasion. Trends Cell Biol. 1998; 8: 101–106
  • Klemke R. L., Leng J., Molander R., Brooks P. C., Vuori K., Cheresh D. A. CAS/Crk coupling serves as a “Molecular Switch” for induction of cell migration. J. Cell Biol. 1998; 140: 961–972
  • Kornberg L. J., Earp H. S., Turner C. E., Prockop C., Juliano R. L. Signal transduction by integrins: increased protein tyrosine phosphorylation caused by clustering of β1 integrins. Proc. Nail. Acad. Sci. USA 1991; 88: 8392–8396
  • Lin E. C.K., Ratnikov B. I., Tsai P. M., Carron C. P., Myers D. M., Barbas C.F., Smith J. Identification of a region in the integrin β3 subunit that confers ligand binding specificity. J. Biol. Chem. 1997; 272: 23912–23920
  • Lipfert L., Haimovich B., Schaller M. D., Cobb B. S., Parsons J. T., Brugge J. S. Integrin-dependent phosphorylation and activation of the protein tyrosine kinase pp125FAKin platelets. J. Cell Biol. 1992; 119: 905–912
  • Marcinkiewicz C., Calvete J. J., Marcinkiewicz M. M., Raida M., Vijay-Kumar S., Huang Z., Lobb R. R., Niewiarowski S. EC3, a novel heterodimeric disintegrin fromEchis carinatusvenom, inhibits α4 and α5 integrins in an RGD-independent manner. J. Biol. Chem. 1999a; 274: 12468–12473
  • Marcinkiewicz C., Calvete J. J., Vijay-Kumar S., Marcinkiewicz M. M., Raida M., Schick P., Lobb R. R., Niewiarowski S. Structural and functional characterization of EMF10, a heterodimeric disintegrin from eristocophis macmahoni venom that selectively inhibits alpha 5 beta 1 integrin. Biochemistry 1999b; 38: 13302–13309
  • Markland F. S., Zhou Q. Snake venom disintegrin: an effective inhibitor of breast cancer growth and dissemination. Natural and Selected Synthetic Toxins: Biological Implications, A. T. Tu, W. Gaffield. American Chemical Society, Washington, DC 2000; 262–282, ACS Symposium Series #745
  • Miyamoto S., Teramoto H., Coso O. A., Gutkind J. S., Burbelo P. D., Akiyama S. K., Yamada K. M. Integrin function: molecular hierarchies of cytoskeletal and signaling molecules. J. Cell Biol. 1995; 131: 791–805
  • Niewiarowski S., McLane M. A., Kloczewiak M., Stewart G. Disintegrins and other naturally occurring antagonists of platelet fibrinogen receptors. Seminars in Hematology 1994; 31: 289–300
  • Oktay M., Wary K. K., Dans M., Birge R. B., Giancotti F. G. Integrin-mediated activation of focal adhesion kinase is required for signaling to Jun NH2-terminal kinase and progression through the Gl phase of the cell cycle. J. Cell Biol. 1999; 145: 1461–1469
  • Oshikawa K., Terada S. Ussuristatin 2, a novel KGD-bearing disintegrin from Agkistrodon ussuriensis venom. J. Biochem. 1999; 125: 31–35
  • Petitclerc E., Stromblad S., Von Schalscha T. L., Mitjans F., Piulats J., Montgomery A. M., Cheresh D. A., Brooks P. C. lntegrin alpha(v)beta3 promotes M21 melanoma growth in human skin by regulating tumor cell survival. Cancer Research 1999; 59: 2724–2730
  • Pfaff M., McLane M. A., Beviglia L., Niewiarowski S., Timpl R. Comparison of disintegrins with limited variation in the RGO loop in their binding to purified integ-rins αIIbβ3, αvβ3 and α5β1 and in cell adhesion inhibition. Cell Adhesion Commun. 1994; 2: 491–501
  • Price J. E., Polyzos A., Zhang R. D., Daniels L. M. Tumorigenicity and Metastasis of human breast carcinoma cell Lines in nude mice. Cancer Research 1990; 50: 717–721
  • Repesh L. A. A Newin vitroAssay for quantitating tumor cell invasion. Invasion and Metastasis 1989; 9: 192–208
  • Ritter M. R., Markland F. S. Differential regulation of tyrosine phosphorylation in tumor cells by contortrostatin, a homodimeric disintegrin, and monomelic disintegrins echistatin and flavoridin. Toxicon 2001; 39: 283–289
  • Ritter M. R., Zhou Q., Markland F. S. Contortrostatin, A snake venom disintegrin, induces αvβ3-mediated tyrosine phosphorylation of CAS and FAK in tumor cells. J. Cell. Biochem. 2000; 79: 28–37
  • Rodriguez-Fernandez J. L. Why do so many stimuli induce tyrosine phosphorylation of FAK?. Bioessays 1999; 21: 1069–1075
  • Schaller M. D., Hildebrand J. D., Shannon J. D., Fox J. W., Vines R. R., Parsons J. T. Autophosphorylation of the focal adhesion kinase, pp125FAKdirects SH2-dependent binding of pp60src. Mol. Cell. Biol. 1994; 14: 1680–1688
  • Schilder R. J., Hall L., Monks A., Handel L. M., Fornace A. J., Jr., Ozols R. F., Fqjo A. T., Hamilton T. C. Metallothionein gene expression and resistance to cisplatin in human ovarian cancer. International J. Cancer 1990; 45: 416–422
  • Schlaepfer D. D., Hanks S. K., Hunter T., van der Geer P. Integrin-mediated signal transduction linked to Ras pathway by GRB2 binding to focal adhesion kinase. Nature 1994; 372: 786–791
  • Schlaepfer D. D., Jones K. C., Hunter T. Multiple Grb2-mediated integrin-stimulated signaling pathways to ERK2/mitogen-activated protein kinase: summation of both c-Src- and focal adhesion kinase-initiated tyrosine phosphorylation events. Mol. Cell. Biol. 1998; 18: 2571–2585
  • Senn H., Klaus W. The nuclear magnetic resonance solution structure of flavoridin, an antagonist of the platelet GP IIb-IIIa receptor. J. Mol. Biol. 1993; 232: 907–925
  • Sheu J.-R., Yen M.-H., Kan Y.-C., Hung W.-C., Chang P.-T., Luk H.-N. Inhibition of angiogenesisin vitroandin vivo: comparison of the relative activities of triflavin, an Arg-Gly-Asp-containing peptide and anti-αvβ3 integrin monoclonal antibody. Biochim. Biophys. Acta 1997; 1336: 445–454
  • Sieg D. J., Hauck C. R., Schlaepfer D. D. Required role of focal adhesion kinase (FAK) for integrin-stimulated cell migration. J. Cell Sci. 1999; 112: 2677–2691
  • Tam S. H., Sassoli P. M., Jordan R. E., Nakada M. T. Abciximab (ReoPro, chimeric 7E3 Fab) demonstrates equivalent affinity and functional blockade of GPIIb/IIIa and αvβ3 integrins. Circulation 1998; 98: 1085–1091
  • Trikha M., de Clerck Y. A., Markland F. S. Contortrostatin, a snake venom disintegrin, inhibits β1 integrin-mediated human metastatic melanoma cell adhesion and blocks experimental metastasis. Cancer Research 1994a; 54: 4993–4998
  • Trikha M., Rote W., Manley P., Lucchesi B. R., Mark-Land F. S. Purification and characterization of platelet aggregation inhibitors from snake venoms. Thrombosis Research 1994b; 73: 39–52
  • Vuori K., Hirai H., Aizawa S., Ruoslahti E. Induction of p130cassignaling complex formation upon integrin-mediated cell adhesion: a role for Src family kinases. Mol. Cell. Biol. 1996; 16: 2606–2613
  • Wong N. C., Mueller B. M., Barbas C. F., Ruminski P., Quaranta V., Lin E. C., Smith J. W. Alpha v integrins mediate adhesion and migration of breast carcinoma cell lines. Clinical Exp. Metastasis 1998; 16: 50–61
  • Yamakita Y., Totsukawa G., Yamashiro S., Fry D., Zhang X., Hanks S. K., Matsumura F. Dissociation of FAK/p130(CAS)/c-Src complex during mitosis: role of mitosis-specific serine phosphorylation of FAK. J. Cell Biol. 1999; 144: 315–324
  • Yeh C. H., Peng H.-C., Huang T.-F. Accutin, a new disintegrin, inhibits angiogenesisin vitroandin vivoby acting as integrin αvβ3 antagonist and inducing apoptosis. Blood 1998; 92: 3268–3276
  • Zhou Q., Nakada M., Arnold C., Shieh K. Y., Markland F. S. Contortrostatin, a dimeric disintegrin fromAgkistrodon contortrix contortrixinhibits angiogenesis. Angiogenesis 1999; 3: 259–269
  • Zhou Q., Hu P., Ritter M. R., Swenson S. D., Argounova S., Epstein A. L., Markland F. S. Molecular cloning and functional expression of contortrostatin, a homodimeric disintegrin from southern copperhead snake venom. Archs Biochem. Biophys. 2000a; 375: 278–288
  • Zhou Q., Nakada M. T., Brooks P.C, Swenson S. D., Ritter M. R., Argounova S., Arnold C., Markland F. S. Contortrostatin, a homodimeric disintegrin, binds to integrin αvβ5. Biochem. Biophys. Res. Commun. 2000b; 267: 350–355
  • Zhou Q., Sherwin R. P., Parrish C., Richters V., Groshen S. G., Tsao-Wei D., Markland F. S. Contortrostatin, a dimeric disintegrin from Agkistrodon contortrix contortrix, inhibits breast cancer progression. Breast Cancer Res. Treat. 2000c; 61(3)249–260

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.