370
Views
7
CrossRef citations to date
0
Altmetric
ORIGINAL ARTICLE

Optimizing the Solution Conditions to Solve the Structure of the Connexin43 Carboxyl Terminus Attached to the 4th Transmembrane Domain in Detergent Micelles

, &
Pages 23-33 | Received 07 Dec 2009, Accepted 25 Mar 2010, Published online: 01 Jun 2010

REFERENCES

  • Columbus L, Lipfert J, Jambunathan K, Fox DA, Sim AY, Doniach S, Lesley SA (2009). Mixing and matching detergents for membrane protein NMR structure determination. J Am Chem Soc. 131: 7320–7326.
  • Delaglio F, Grzesiek S, Vuister GW, Zhu G, Pfeifer J, Bax A (1995). NMRPipe: A multidimensional spectral processing system based on UNIX pipes. J Biomol NMR. 6: 277–293.
  • Delmar M, Coombs W, Sorgen P, Duffy HS, Taffet SM (2004). Structural bases for the chemical regulation of Connexin43 channels. Cardiovasc Res. 62: 268–275.
  • Dobrowolski R, Willecke K (2009). Connexin-caused genetic diseases and corresponding mouse models. Antioxid Redox Signal. 11: 283–295.
  • Duffy HS, Ashton AW, O'Donnell P, Coombs W, Taffet SM, Delmar M, Spray DC (2004). Regulation of connexin43 protein complexes by intracellular acidification. Circ Res. 94: 215–222.
  • Duffy HS, Sorgen PL, Girvin ME, O'Donnell P, Coombs W, Taffet SM, Delmar M, Spray DC (2002). pH-dependent intramolecular binding and structure involving Cx43 cytoplasmic domains. J Biol Chem. 277: 36706–36714.
  • Fernandez C, Wuthrich K (2003). NMR solution structure determination of membrane proteins reconstituted in detergent micelles. FEBS Lett. 555: 144–150.
  • Fort AG, Spray DC (2009). Trifluoroethanol reveals helical propensity at analogous positions in cytoplasmic domains of three connexins. Biopolymers. 92: 173–182.
  • Hirst-Jensen BJ, Sahoo P, Kieken F, Delmar M, Sorgen PL (2007). Characterization of the pH-dependent interaction between the gap junction protein connexin43 carboxyl terminus and cytoplasmic loop domains. J Biol Chem. 282:5801–5813.
  • Johnson BA (2004). Using NMRView to visualize and analyze the NMR spectra of macromolecules. Methods Mol Biol. 278: 313–352.
  • Kellezi A, Grosely R, Kieken F, Borgstahl GE, Sorgen PL (2008). Purification and reconstitution of the connexin43 carboxyl terminus attached to the 4th transmembrane domain in detergent micelles. Protein Expr Purif. 59: 215–222.
  • Krueger-Koplin RD, Sorgen PL, Krueger-Koplin ST, Rivera-Torres IO, Cahill SM, Hicks DB, Grinius L, Krulwich TA, Girvin ME (2004). An evaluation of detergents for NMR structural studies of membrane proteins. J Biomol NMR. 28: 43–57.
  • Lees JG, Smith BR, Wien F, Miles AJ, Wallace BA (2004). CDtool—An integrated software package for circular dichroism spectroscopic data processing, analysis, and archiving. Anal Biochem. 332: 285–289.
  • Libich DS, Harauz G (2008). Solution NMR and CD spectroscopy of an intrinsically disordered, peripheral membrane protein: Evaluation of aqueous and membrane-mimetic solvent conditions for studying the conformational adaptability of the 18.5 kDa isoform of myelin basic protein (MBP). Eur Biophys J. 37: 1015–1029.
  • Lipari G, Szabo A (1981). Nuclear magnetic resonance relaxation in nucleic acid fragments: Models for internal motion. Biochemistry. 20: 6250–6256.
  • Maeda S, Nakagawa S, Suga M, Yamashita E, Oshima A, Fujiyoshi Y, Tsukihara T (2009). Structure of the connexin 26 gap junction channel at 3.5 Å resolution. Nature. 458: 597–602.
  • Main ER, Jackson SE (1999). Does trifluoroethanol affect folding pathways and can it be used as a probe of structure in transition states? Nat Struct Biol. 6: 831–835.
  • Orekhov VYu, Nolde DE, Golovanov AP, Korzhnev DM, Arseniev AS (1995). Processing of heteronuclear NMR relaxation data with the new software DASHA. Appl Magn Reson. 9:581–588.
  • Reiersen H, Rees AR (2000). Trifluoroethanol may form a solvent matrix for assisted hydrophobic interactions between peptide side chains. Protein Eng. 13: 739–743.
  • Sanders CR, Sonnichsen F (2006). Solution NMR of membrane proteins: Practice and challenges. Magn Reson Chem. 44(Spec No.): S24–S40.
  • Severs NJ, Bruce AF, Dupont E, Rothery S (2008). Remodelling of gap junctions and connexin expression in diseased myocardium. Cardiovasc Res. 80: 9–19.
  • Sorgen PL, Duffy HS, Sahoo P, Coombs W, Delmar M, Spray DC (2004a). Structural changes in the carboxyl terminus of the gap junction protein connexin43 indicates signaling between binding domains for c-Src and zonula occludens-1. J Biol Chem. 279: 54695–54701.
  • Sorgen PL, Duffy HS, Spray DC, Delmar M (2004b). pH-dependent dimerization of the carboxyl terminal domain of Cx43. Biophys J. 87: 574–581.
  • Unger VM, Kumar NM, Gilula NB, Yeager M (1999a). Expression, two-dimensional crystallization, and electron cryo-crystallography of recombinant gap junction membrane channels. J Struct Biol. 128: 98–105.
  • Unger VM, Kumar NM, Gilula NB, Yeager M (1999b). Three-dimensional structure of a recombinant gap junction membrane channel. Science. 283: 1176–1180.
  • Weber DJ, Gittis AG, Mullen GP, Abeygunawardana C, Lattman EE, Mildvan AS (1992). NMR docking of a substrate into the X-ray structure of staphylococcal nuclease. Proteins. 13: 275–287.
  • Whitmore L, Wallace BA (2008). Protein secondary structure analyses from circular dichroism spectroscopy: Methods and reference databases. Biopolymers. 89: 392–400.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.