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Original Article

Long-term exposures to low doses of titanium dioxide nanoparticles induce cell transformation, but not genotoxic damage in BEAS-2B cells

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Pages 568-578 | Received 24 Jan 2014, Accepted 08 Aug 2014, Published online: 19 Sep 2014

References

  • Andersson PO, Lejon C, Ekstrand-Hammarström B, Akfur C, Ahlinder L, Bucht A, Osterlund L. 2011. Polymorph- and size-dependent uptake and toxicity of TiO- nanoparticles in living lung epithelial cells. Small 7:514–23
  • Armand L, Dagouassat M, Belade E, Simon-Deckers A, Le Gouvello S, Tharabat C, et al. 2013. Titanium dioxide nanoparticles induce matrix metalloprotease 1 in human pulmonary fibroblasts partly via an interleukin-1β-dependent mechanism. Am J Respir Cell Mol Biol 48:354–63
  • Arora S, Rajwade JM, Paknikar KM. 2012. Nanotoxicology and in vitro studies: the need of the hour. Toxicol Appl Pharmacol 258:151–65
  • Annangi B, Bach J, Vales G, Rubio L, Marcos R, Hernández A. 2014. Long-term exposures to low doses of cobalt nanoparticles induce cell-transformation enhanced by oxidative damage. Nanotoxicology DOI: 10.3109/17435390.2014.900582
  • Barrett JC, Ts'o PO. 1978. Evidence for the progressive nature of neoplastic transformation in vitro. Proc Natl Acad Sci USA 75:3761–5
  • Becker H, Herzberg F, Schulte A, Kolossa-Gehring M. 2011. The carcinogenic potential of nanomaterials, their release from products and options for regulating them. Int J Hyg Environ Health 214:231–8
  • Bhattacharya K, Davoren M, Boertz J, Schins RP, Hoffmann E, Dopp E. 2009. Titanium dioxide nanoparticles induce oxidative stress and DNA-adduct formation but not DNA-breakage in human lung cells. Part Fibre Toxicol 6:17
  • Collins AR. 2004. The comet assay for DNA damage and repair: principles, applications, and limitations. Mol Biotechnol 26:249–61
  • Dusinska M, Collins AR. 2008. The comet assay in human biomonitoring: gene–environment interactions. Mutagenesis 23:191–205
  • Falck GC, Lindberg HK, Suhonen S, Vippola M, Vanhala E, Catalán J, Savolainen K, Norppa H. 2009. Genotoxic effects of nanosized and fine TiO2. Hum Exp Toxicol 28:339–52
  • Fenech M. 2007. Cytokinesis-block micronucleus cytome assay. Nat Protoc 2:1084–104
  • Gibbs-Flournoy EA, Bromberg PA, Hofer TP, Samet JM, Zucker RM. 2011. Darkfield-confocal microscopy detection of nanoscale particle internalization by human lung cells. Part Fibre Toxicol 8:2
  • Ghosh M, Chakraborty A, Mukherjee A. 2013. Cytotoxic, genotoxic and the hemolytic effect of titanium dioxide (TiO2) nanoparticles on human erythrocyte and lymphocyte cells in vitro. J Appl Toxicol 33:1097–110
  • Gonçalves DM, Chiasson S, Girard D. 2010. Activation of human neutrophils by titanium dioxide (TiO2) nanoparticles. Toxicol In Vitro 24:1002–8
  • Hackenberg S, Scherzed A, Technau A, Froelich K, Hagen R, Kleinsasser N. 2013. Functional responses of human adipose tissue-derived mesenchymal stem cells to metal oxide nanoparticles in vitro. J Biomed Nanotechnol 9:86–95
  • Hamzeh M, Sunahara GI. 2013. In vitro cytotoxicity and genotoxicity studies of titanium dioxide (TiO2) nanoparticles in Chinese hamster lung fibroblast cells. Toxicol In Vitro 27:864–73
  • Hernández LG, van Steeg H, Luijten M, van Benthem J. 2009. Mechanisms of non-genotoxic carcinogens and importance of a weight of evidence approach. Mutat Res 682:94–109
  • Hristozov DR, Gottardo S, Critto A, Marcomini A. 2012. Risk assessment of engineered nanomaterials: a review of available data and approaches from a regulatory perspective. Nanotoxicology 6:880–98
  • Huang S, Chueh PJ, Lin YW, Shih TS, Chuang SM. 2009. Disturbed mitotic progression and genome segregation are involved in cell transformation mediated by nano-TiO2 long-term exposure. Toxicol Appl Pharmacol 241:182–94
  • Jacobsen NR, Saber AT, White P, Møller P, Pojana G, Vogel U, et al. 2007. Increased mutant frequency by carbon black, but not quartz, in the lacZ and cII transgenes of muta mouse lung epithelial cells. Environ Mol Mutagen 48:451–61
  • Kang SJ, Kim BM, Lee YJ, Chung HW. 2008. Titanium dioxide nanoparticles trigger p53-mediated damage response in peripheral blood lymphocytes. Environ Mol Mutagen 49:399–405
  • Kocbek P, Teskac K, Kreft ME, Kristl J. 2010. Toxicological aspects of long-term treatment of keratinocytes with ZnO and TiO2 nanoparticles. Small 6:1908–17
  • Lindberg HK, Falck GC, Catalán J, Koivisto AJ, Suhonen S, Järventaus H, et al. 2012. Genotoxicity of inhaled nanosized TiO2 in mice. Mutat Res 745:58–64
  • Liu Q, Hong Z, Guo B, Zhang Y, Li Y, Liu J. 2006. Experimental study on toxicity of nanozided titanium dioxide. Mod Preventive Med 33:1211–12
  • Magdolenova Z, Bilaničová D, Pojana G, Fjellsbø LM, Hudecova A, Hasplova K, et al. 2012a. Impact of agglomeration and different dispersions of titanium dioxide nanoparticles on the human related in vitro cytotoxicity and genotoxicity. J Environ Monit 14:455–64
  • Magdolenova Z, Lorenzo Y, Collins A, Dusinska M. 2012b. Can standard genotoxicity tests be applied to nanoparticles? J Toxicol Environ Health A 75:800–6
  • McNamee JP, McLean JR, Ferrarotto CL, Bellier PV. 2000. Comet assay: rapid processing of multiple samples. Mutat Res 466:63–9
  • Mussá T, Rodríguez-Cariño C, Sánchez-Chardi A, Baratelli M, Costa-Hurtado M, Fraile L, et al. 2012. Differential interactions of virulent and nonvirulent H. parasuis strains with naïve or swine influenza virus pre-infected dendritic cells. Vet Res 43:80
  • Nanogenotox, 2011. Available from: http://www.nanogenotox.eu/files/PDF/Deliverables/nanogenotox %20deliverable%203_wp4_%20dispersion%20protocol.pdf
  • Nanogenotox, 2012. Available from: http://www.nanogenotox.eu/files/PDF/Deliverables/d4.5_zeta_dls_ saxs_analysis.pdf
  • Oum'hamed Z, Garnotel R, Josset Y, Trenteseaux C, Laurent-Maquin D. 2004. Matrix metalloproteinases MMP-2, -9 and tissue inhibitors TIMP-1, -2 expression and secretion by primary human osteoblast cells in response to titanium, zirconia, and alumina ceramics. J Biomed Mater Res A 68:114–22
  • Park EJ, Yi J, Chung KH, Ryu DY, Choi J, Park K. 2008. Oxidative stress and apoptosis induced by titanium dioxide nanoparticles in cultured BEAS-2B cells. Toxicol Lett 180:222–9
  • Petković J, Zegura B, Stevanović M, Drnovšek N, Uskoković D, Novak S, Filipič M. 2011. DNA damage and alterations in expression of DNA damage responsive genes induced by TiO2 nanoparticles in human hepatoma HepG2 cells. Nanotoxicology 5:341–53
  • Prasad RY, Wallace K, Daniel KM, Tennant AH, Zucker RM, Strickland J, et al. 2013. Effect of treatment media on the agglomeration of titanium dioxide nanoparticles: impact on genotoxicity, cellular interaction, and cell cycle. ACS Nano 7:1929–42
  • Rodríguez-Cariño C, Duffy C, Sánchez-Chardi A, McNeilly F, Allan GM, Segalés J. 2011. Porcine circovirus type 2 morphogenesis in a clone derived from the l35 lymphoblastoid cell line. J Comp Pathol 144:91–102
  • Shi H, Magaye R, Castranova V, Zhao J. 2013. Titanium dioxide nanoparticles: a review of current toxicological data. Part Fibre Toxicol 10:15
  • Singh S, Shi T, Duffin R, Albrecht C, van Berlo D, Höhr D, et al. 2007. Endocytosis, oxidative stress and IL-8 expression in human lung epithelial cells upon treatment with fine and ultrafine TiO2: role of the specific surface area and of surface methylation of the particles. Toxicol Appl Pharmacol 222:141–51
  • Sun H, Clancy HA, Kluz T, Zavadil J, Costa M. 2011. Comparison of gene expression profiles in chromate transformed BEAS-2B cells. PLoS One 6:e17982
  • Surrallés J, Xamena N, Creus A, Catalán J, Norppa H, Marcos R. 1995. Induction of micronuclei by five pyrethroid insecticides in whole blood and isolated human lymphocyte cultures. Mutat Res 341:169–84
  • Suzuki H, Toyooka T, Ibuki Y. 2007. Simple and easy method to evaluate uptake potential of nanoparticles in mammalian cells using a flow cytometric light scatter analysis. Environ Sci Technol 41:3018–24
  • Toduka Y, Toyooka T, Ibuki Y. 2012. Flow cytometric evaluation of nanoparticles using side-scattered light and reactive oxygen species-mediated fluorescence–correlation with genotoxicity. Environ Sci Technol 4614:762976–36
  • Vanparys P, Corvi R, Aardema MJ, Gribaldo L, Hayashi M, Hoffmann S, Schechtman L. 2012. Application of in vitro cell transformation assays in regulatory toxicology for pharmaceuticals, chemicals, food products and cosmetics. Mutat Res 744:111–16
  • Xu J, Futakuchi M, Iigo M, Fukamachi K, Alexander DB, Shimizu H, et al. 2010. Involvement of macrophage inflammatory protein 1alpha (MIP1alpha) in promotion of rat lung and mammary carcinogenic activity of nanoscale titanium dioxide particles administered by intra-pulmonary spraying. Carcinogenesis 31:927–35
  • Xu J, Sagawa Y, Futakuchi M, Fukamachi K, Alexander DB, Furukawa F, et al. 2011. Lack of promoting effect of titanium dioxide particles on ultraviolet B-initiated skin carcinogenesis in rats. Food Chem Toxicol 49:1298–302
  • Xu J, Shi H, Ruth M, Yu H, Lazar L, Zou B, et al. 2013. Acute toxicity of intravenously administered titanium dioxide nanoparticles in mice. PLoS One 8:e70618

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