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ORIGINAL ARTICLES

Identification of novel in vitro protein kinase A phosphorylation sites on recombinant non-muscle myosin light chain kinase: nano-liquid chromatography tandem mass spectrometry methodology

, , , , & , MD
Pages 242-253 | Published online: 18 Nov 2009

References

  • Hunter T. Protein kinases and phosphatases: The yin and yang of protein phosphorylation and signaling. Cell 1995; 80: 225–36
  • Deng JT, Van Lierop JE, Sutherland C, Walsh MP. Ca2+-independent smooth muscle contraction. A novel function for integrin-linked kinase. J Biol Chem 2001; 276: 16365–73
  • Tinsley JH, De Lanerolle P, Wilson E, Ma W, Yuan SY. Myosin light chain kinase transference induces myosin light chain activation and endothelial hyperpermeability. Am J Physiol Cell Physiol 2000; 279: C1285–9
  • Garcia JG, Schaphorst KL. Regulation of endothelial cell gap formation and paracellular permeability. J Invest Med 1995; 43: 117–26
  • Garcia JG, Davis HW, Patterson CE. Regulation of endothelial cell gap formation and barrier dysfunction: Role of myosin light chain phosphorylation. J Cell Physiol 1995; 163: 510–22
  • Garcia JG, Lazar V, Gilbert-McClain LI, Gallagher PJ, Verin AD. Myosin light chain kinase in endothelium: molecular cloning and regulation. Am J Respir Cell Mol Biol 1997; 16: 489–94
  • Essler M, Amano M, Kruse HJ, Kaibuchi K, Weber PC, Aepfelbacher M. Thrombin inactivates myosin light chain phosphatase via rho and its target rho kinase in human endothelial cells. J Biol Chem 1998; 273: 21867–74
  • Carbajal JM, Gratrix ML, Yu CH, Schaeffer RC, Jr. Rock mediates thrombin′s endothelial barrier dysfunction. Am J Physiol Cell Physiol 2000; 279: C195–204
  • Dudek SM, Garcia JG. Cytoskeletal regulation of pulmonary vascular permeability. J Appl Physiol 2001; 91: 1487–500
  • Garcia JG, Verin AD, Herenyiova M, English D. Adherent neutrophils activate endothelial myosin light chain kinase: role in transendothelial migration. J Appl Physiol 1998; 84: 1817–21
  • Petrache I, Verin AD, Crow MT, Birukova A, Liu F, Garcia JG. Differential effect of mlc kinase in tnf-alpha-induced endothelial cell apoptosis and barrier dysfunction. Am J Physiol Lung Cell Mol Physiol 2001; 280: L1168–78
  • Birukov KG, Birukova AA, Dudek SM, Verin AD, Crow MT, Zhan X, et al. Shear stress-mediated cytoskeletal remodeling and cortactin translocation in pulmonary endothelial cells. Am J Respir Cell Mol Biol 2002; 26: 453–64
  • Linz-McGillem LA, Moitra J, Garcia JG. Cytoskeletal rearrangement and caspase activation in sphingosine 1-phosphate-induced lung capillary tube formation. Stem Cells Dev 2004; 13: 496–508
  • Wadgaonkar R, Linz-McGillem L, Zaiman AL, Garcia JG. Endothelial cell myosin light chain kinase (mlck) regulates tnfalpha-induced nfkappab activity. J Cell Biochem 2005; 94: 351–64
  • Wainwright MS, Rossi J, Schavocky J, Crawford S, Steinhorn D, Velentza AV, et al. Protein kinase involved in lung injury susceptibility: Evidence from enzyme isoform genetic knockout and in vivo inhibitor treatment. Proc Natl Acad Sci U S A 2003; 100: 6233–8
  • Gao L, Grant A, Halder I, Brower R, Sevransky J, Maloney JP, et al. Novel polymorphisms in the myosin light chain kinase gene confer risk for acute lung injury. Am J Respir Cell Mol Biol 2006; 34: 487–95
  • Lazar V, Garcia JG. A single human myosin light chain kinase gene (mlck; mylk). Genomics 1999; 57: 256–67
  • Garcia JG, Verin AD, Schaphorst K, Siddiqui R, Patterson CE, Csortos C, et al. Regulation of endothelial cell myosin light chain kinase by rho, cortactin, and p60(src). Am J Physiol 1999; 276: L989–98
  • Wadgaonkar R, Dudek SM, Zaiman AL, Linz-McGillem L, Verin AD, Nurmukhambetova S, et al. Intracellular interaction of myosin light chain kinase with macrophage migration inhibition factor (mif) in endothelium. J Cell Biochem 2005; 95: 849–58
  • Dudek SM, Birukov KG, Zhan X, Garcia JG. Novel interaction of cortactin with endothelial cell myosin light chain kinase. Biochem Biophys Res Commun 2002; 298: 511–9
  • Verin AD, Gilbert-McClain LI, Patterson CE, Garcia JG. Biochemical regulation of the nonmuscle myosin light chain kinase isoform in bovine endothelium. Am J Respir Cell Mol Biol 1998; 19: 767–76
  • Patterson CE, Lum H, Schaphorst KL, Verin AD, Garcia JG. Regulation of endothelial barrier function by the camp-dependent protein kinase. Endothelium 2000; 7: 287–308
  • Krymsky MA, Kudryashov DS, Shirinsky VP, Lukas TJ, Watterson DM, Vorotnikov AV. Phosphorylation of kinase-related protein (telokin) in tonic and phasic smooth muscles. J Muscle Res Cell Motil 2001; 22: 425–37
  • MacDonald JA, Walker LA, Nakamoto RK, Gorenne I, Somlyo AV, Somlyo AP, et al. Phosphorylation of telokin by cyclic nucleotide kinases and the identification of in vivo phosphorylation sites in smooth muscle. FEBS Lett 2000; 479: 83–8
  • Wu X, Haystead TA, Nakamoto RK, Somlyo AV, Somlyo AP. Acceleration of myosin light chain dephosphorylation and relaxation of smooth muscle by telokin. Synergism with cyclic nucleotide-activated kinase. J Biol Chem 1998; 273: 11362–9
  • Birukov KG, Csortos C, Marzilli L, Dudek S, Ma SF, Bresnick AR, et al. Differential regulation of alternatively spliced endothelial cell myosin light chain kinase isoforms by p60(src). J Biol Chem 2001; 276: 8567–73
  • Carbajal JM, Schaeffer RC, Jr. H2O2 and genistein differentially modulate protein tyrosine phosphorylation, endothelial morphology, and monolayer barrier function. Biochem Biophys Res Commun 1998; 249: 461–6
  • Goeckeler ZM, Masaracchia RA, Zeng Q, Chew TL, Gallagher P, Wysolmerski RB. Phosphorylation of myosin light chain kinase by p21-activated kinase pak2. J Biol Chem 2000; 275: 18366–74
  • Wolff JC, Eckers C, Sage AB, Giles K, Bateman R. Accurate mass liquid chromatography/mass spectrometry on quadrupole orthogonal acceleration time-of-flight mass analyzers using switching between separate sample and reference sprays. 2. Applications using the dual-electrospray ion source. Anal Chem 2001; 73: 2605–12
  • Larsen MR, Thingholm TE, Jensen ON, Roepstorff P, Jorgensen TJ. Highly selective enrichment of phosphorylated peptides from peptide mixtures using titanium dioxide microcolumns. Mol Cell Proteomics 2005; 4: 873–86
  • Hannis JC, Muddiman DC. A dual electrospray ionization source combined with hexapole accumulation to achieve high mass accuracy of biopolymers in fourier transform ion cyclotron resonance mass spectrometry. J Am Soc Mass Spectrom 2000; 11: 876–83
  • Yates JR 3rd, Eng JK, McCormack AL, Schieltz D. Method to correlate tandem mass spectra of modified peptides to amino acid sequences in the protein database. Anal Chem 1995; 67: 1426–36
  • Schroeder MJ, Webb DJ, Shabanowitz J, Horwitz AF, Hunt DF. Methods for the detection of paxillin post-translational modifications and interacting proteins by mass spectrometry. J Proteome Res 2005; 4: 1832–41
  • Hoffert JD, Pisitkun T, Wang G, Shen RF, Knepper MA. Quantitative phosphoproteomics of vasopressin-sensitive renal cells: Regulation of aquaporin-2 phosphorylation at two sites. Proc Natl Acad Sci U S A 2006; 103: 7159–64
  • Blom N, Sicheritz-Ponten T, Gupta R, Gammeltoft S, Brunak S. Prediction of post-translational glycosylation and phosphorylation of proteins from the amino acid sequence. Proteomics 2004; 4: 1633–49
  • Hjerrild M, Stensballe A, Rasmussen TE, Kofoed CB, Blom N, Sicheritz-Ponten T, et al. Identification of phosphorylation sites in protein kinase a substrates using artificial neural networks and mass spectrometry. J Proteome Res 2004; 3: 426–33
  • Kobe B, Kampmann T, Forwood JK, Listwan P, Brinkworth RI. Substrate specificity of protein kinases and computational prediction of substrates. Biochim Biophys Acta 2005; 1754: 200–9
  • Brinkworth RI, Breinl RA, Kobe B. Structural basis and prediction of substrate specificity in protein serine/threonine kinases. Proc Natl Acad Sci U S A 2003; 100: 74–9
  • Xue Y, Li A, Wang L, Feng H, Yao X. Ppsp: prediction of pk-specific phosphorylation site with Bayesian decision theory. BMC Bioinformatics 2006; 7: 163
  • Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villen J, Li J, et al. Large-scale characterization of hela cell nuclear phosphoproteins. Proc Natl Acad Sci U S A 2004; 101: 12130–5
  • Molloy MP, Andrews PC. Phosphopeptide derivatization signatures to identify serine and threonine phosphorylated peptides by mass spectrometry. Anal Chem 2001; 73: 5387–94
  • Garcia JG, Wang P, Liu F, Hershenson MB, Borbiev T, Verin AD. Pertussis toxin directly activates endothelial cell p42/p44 map kinases via a novel signaling pathway. Am J Physiol Cell Physiol 2001; 280: C1233–41
  • Garcia JG, Liu F, Verin AD, Birukova A, Dechert MA, Gerthoffer WT, et al. Sphingosine 1-phosphate promotes endothelial cell barrier integrity by Edg-dependent cytoskeletal rearrangement. J Clin Invest 2001; 108: 689–701
  • Liu F, Verin AD, Wang P, Day R, Wersto RP, Chrest FJ, et al. Differential regulation of sphingosine-1-phosphate- and vegf-induced endothelial cell chemotaxis. Involvement of g(ialpha2)-linked rho kinase activity. Am J Respir Cell Mol Biol 2001; 24: 711–9
  • Khapchaev AY, Krymsky MA, Sidorova MV, Bespalova Zh D, Wang CL, Shirinsky VP, et al. Novel phosphospecific antibodies for monitoring phosphorylation of proteins encoded by the myosin light chain kinase genetic locus. Biochemistry 2004; 69: 789–98
  • Foyt HL, Means AR. Characterization and analysis of an apparent autophosphorylation of chicken gizzard myosin light chain kinase. J Cyclic Nucleotide Protein Phosphor Res 1985; 10: 143–55
  • Tokui T, Ando S, Ikebe M. Autophosphorylation of smooth muscle myosin light chain kinase at its regulatory domain. Biochemistry 1995; 34: 5173–9

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