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Abstract
Ginsenosides Re and Rg1, the main bioactive components of Panax ginseng, have been preparatively isolated and purified using high-speed counter-current chromatography (HSCCC) combined with MCI gel column chromatography (MCI gel CC). Guided by HPLC-MS, the crude extracts from the roots of P. ginseng were pre-separated on an MCI gel column to obtain mixtures of ginsenosides Re and Rg1. Then, a 200 mg volume of enriched sample was separated by HSCCC with a two-phase solvent system composed of ethyl acetate–n-butanol–water (4: 1: 6, v/v/v), yielding 52.6 mg of Rg1 and 37.2 mg of Re with the purities of 99.3% and 98.2%, respectively. The total recovery of Rg1 and Re was 82.4% and 84.4%, respectively, in the two-step separation. This study introduces a fast and efficient method for purification of ginsenosides Re and Rg1 from P. ginseng.
ACKNOWLEDGMENT
This research work was financially supported by the National Key Scientific and Technological Special Projects (2009ZX09502-011), the National Natural Science Foundation of China (30830116), and the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions.
Notes
a Partition coefficient (k = Au/Al) is defined as solute peak area in the upper phase divided by that of compound in the lower phase.
b Separation factor between two components is defined as (α = k1/k2, k1 > k2).