Development of 11 microsatellite markers in Pinus parviflora by the dual-suppression technique and next-generation sequencing

Abstract

Pinus parviflora Sieb. et Zucc. var. parviflora is a coniferous tree species distributed in mountainous areas of temperate zones from southern Tohoku to the Kyushu area in Japan. Some P. parviflora populations have become small and endangered due to massive death resulting from pine wilt disease, scab canker, and presumably climate change. We developed 11 microsatellite markers for P. parviflora by the dual-suppression technique and next-generation sequencing using 32 individuals of P. parviflora collected from Aokigahara at the foot of Mt. Fuji. The number of alleles for each locus ranged from two to 10. The averages of observed and expected heterozygosities were 0.61 and 0.59, respectively. These markers will become powerful tools for assessing genetic diversity, genetic connectivity, and genetic structure in P. parviflora populations, which will facilitate our understanding and conservation of P. parviflora.

Keywords:

• Genetic diversity
• NGS
• Population structure
• SSR

Acknowledgments

We thank Professor Y. Watano and Dr. M. Kimura for their kind advice during the early stages of our present study. This study was partly supported by the Fujiwara Natural History Public Interest Incorporated Foundation.