Abstract
Introduction
In recent years, the role of high mobility group box-(HMGB-1) protein and its receptors has received increasing attention. It has been documented that HMGB-1 is associated with disease activity in systemic lupus erythematosus (SLE). HMGB-1 supports the inflammatory clearance of apoptotic cells and remnants. It binds to molecules released from apoptotic cells such as nucleosomes and DNA thereby, increasing the immunogenicity of macrophages through receptors for advanced glycation end products (sRAGE).
Aim of the work
Was to measure the plasma level of sRAGE in SLE patients and to correlate it with the clinical and laboratory parameters of disease activity.
Patients and methods
The study was composed of 35 SLE patients; 31 females and 4 males (Group I) and 20 age and gender matched healthy subjects as a control (Group II). All patients fulfilling the American College of Rheumatology (ACR) classification criteria for the diagnosis of SLE. Active disease was identified using SLE disease activity index (SLE-DAI).
Demographic data, cutaneous manifestations, arthritis, vasculitis, myositis, renal, and hematological disorders were recorded. In addition; complete blood picture, blood urea, serum creatinine, 24 h urine proteins, creatinine clearance, protein/creatinine ratio, C3, C4, Anti-nuclear antibody, Anti-double stranded DNA were conducted for all patients and controls.
Results
The mean value of plasma level of (sRAGE) in SLE patients was significantly higher in SLE patients than in the normal healthy controls (P < 0.001). There was a statistically significant positive correlation between sRAGE and SLE-DAI (P < 0.001).
Conclusion
The plasma level of sRAGE is considered as a potential biomarker for disease activity in SLE, severity and prognosis.
1 Introduction
Systemic lupus erythematosus (SLE) is a chronic autoimmune disease characterized by the involvement of multiple organ systems. Its etiology is largely unknown; however, it has been proposed that genetic and environmental factors contribute to breaking tolerance, resulting in the production of a variety of antibodies directed at self-components.Citation1
These autoantibodies form immune complexes can be deposited in many tissues, particularly the skin and kidneys.Citation2,Citation3 Currently, research is being conducted to determine what patho-physiological mechanisms are involved in this entire process.
Receptor for advanced glycation end products (RAGE) is a multi-ligand member of the immunoglobulin super-family. It is expressed by most types of immune cells, including macrophages, neutrophils, and T cells and interacts with several classes of ligands.Citation4 Currently, the known RAGE ligands include the high mobility group box-1 (HMGB1) protein, advanced glycation end products (AGEs), and members of the S100/calgranulin family.Citation5
One of the proinflammatory mediators is the HMGB1. It was originally recognized as a DNAbinding protein but has recently been identified as a damage-associated molecular pattern (DAMP) molecule.Citation6,Citation7 This nuclear protein participates in chromatin architecture and transcriptional regulation,Citation8 but once released, it induces an inflammatory response.Citation9,Citation10 Extracellular HMGB1 binds to cell surface receptors, including RAGE, toll-like receptors 2 and 4, and others. Studies have shown that interaction between HMGB1 and RAGE results in the production of type -1 interferon, which plays a key role in the pathogenesis of SLE.Citation11,Citation12 In addition, tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) are produced upon HMGB-1 activation of macrophages.Citation13
It has also been hypothesized that these cytokines also affect some body organs as well as disease flareups.Citation14,Citation15 In addition, it has also been postulated that RAGE involvement in all pathophysiological processes is reliant on HMGB1.Citation16 Some studies have reported a relationship between the high serum level of HMGB1 and flare-ups of lupus disease activity.Citation17,Citation18 All of these observations support the notion that the HMBG1–RAGE pathway plays a part in the pathogenesis of SLE.
Another class of ligands is the AGEs. They result from a process in which non-enzymatic glycosylation attaches to circulating compounds such as lipids, proteins, or nucleic acids. This process occurs under the effect of oxidative stress (OS) and hyperglycemia.Citation19 Accumulations of AGEs have been found in certain diseases, including diabetes mellitus (DM) and Alzheimer’s. Since RAGE induction is under the effect of AGEs, the RAGE-AGEs pathway is thought to be incriminated in the pathogenesis of these diseases.Citation20,Citation21
Receptor for advanced glycation end products is a receptor for a family of about 20 related calcium binding proteins that are only expressed in vertebrates. These include the S100s, which are proteins that alter several intracellular functions.Citation22
In addition, many types of body cells release S100s during inflammation; therefore, they can be used as a measure of disease activity.Citation23,Citation24
Soluble RAGE (sRAGE), a truncated form of the receptor, has the same structure but lacks the cytosolic and transmembrane domains. Two general mechanisms are usually responsible for the generation of soluble receptors. These are either derived from the alternative splicing of messenger ribonucleic acid (mRNA) or the cleaved products of the membrane-bound form of metalloproteinase.Citation25,Citation26 Both sRAGE and complete RAGE have the same ligand-binding specificity. Furthermore, sRAGE may act as a trap for pro-inflammatory ligands such as HMGB1 and inhibit their interaction with the RAGE cell surface.Citation27,Citation28
2 Aim of the work
Was to measure the plasma level of sRAGE in SLE patients and to correlate it with the clinical and laboratory parameters of disease activity.
3 Patients and methods
The study was composed of 35 patients with SLE 31 females and 4 males (Group I) and 20 age and gender matched healthy subjects (Group II).
All of the patients conformed to the American College of Rheumatology (ACR) classification criteria for the diagnosis of SLE.Citation29 Active disease was identified using the systemic lupus erythematosus Disease Activity Index (SLE-DAI).Citation30
Demographic and clinical data, including cutaneous manifestations, arthritis, vasculitis, myositis, renal disorders were recorded. In addition; complete blood picture,Citation31 blood urea nitrogen, serum creatinine, 24 h urine proteins, protein/creatinine ratio, creatinine clearance,Citation32 C3, C4,Citation33 anti-nuclear antibody, (ANA),Citation34, and anti-double stranded DNA (Anti-ds DNA)Citation35 were conducted for all patients and controls.
Plasma concentrations of sRAGE levels were measured using enzyme Linked Immunosorbant Assay (ELISA).
Patients with other connective tissue diseases were excluded from the study, and also patients with diabetes, endocrinal diseases, and HCV positive patients.
Written consent was taken from all patients included in the study.
3.1 Statistical analysis of the data
Data were processed and analyzed using a computer based program (SPSS software version 10). The results were expressed as mean ± standard deviation (SD), and comparisons between the SLE patients and the controls were analyzed by the student’s t-test, one way analysis of variance (ANOVA), and spearman’s rank correlation coefficient. A two tailed P-value of <0.05 was considered to be statistically significant.
4 Results
: describes the distribution of the studied cases; 31 cases (88.6%) were females and 4 cases (11.4%) were males. Their age ranged from 18.0 to 52.0 with a mean of 33.60 ± 11.53. The disease duration ranged from 3.0 to 10.0 with a mean of 6.43 ± 2.08. : describes the distribution and frequency of organ involvement in SLE cases; 27 cases (77.1%) presented with arthritis, 14 cases (40.0%) presented with fever and malaise, 13 cases (37.1%) cases presented with myositis, 12 cases (34.3%) presented with pleurisy, 9 cases (25.7%) presented with oral ulcer, 6 cases (17.1%) presented with photosensitivity, 5 cases (14.3%) presented with malar rash, 4 cases (11.4%) presented with psychosis, 4 cases (11.4%) presented with pleurisy, 2 cases (5.7%) presented with hair fall, and 1 case (2.9%) presented with seizures. : describes the laboratory markers of the studied SLE cases; Hb % ranged from 8.0 to 10.50 with a mean of 9.41 ± 1.69, WBCs ranged from 2.40 to 3.51 with a mean of 3.37 ± 2.18, platelet count ranged from 80.0 to 130.0 with a mean of 117.46 ± 64.93, blood urea ranged from 60.0 to 458.0 with a mean of 64.70 ± 7.66, serum creatinine ranged from 2.32 to 8.20 with a mean of 2.50 ± 2.84, creatinine clearance ranged from 95.0 to 130.0 with a mean of 104.30 ± 8.94 and 24-h urine creatinine ranged from 40.0 to 4000.0 with a mean of 1637.43 ± 1587.86. : shows the immunological markers among the studied SLE cases; ANA ranged from 160.0 to 450.0 with a mean of 249.83 ± 230.57, anti-ds-DNA ranged from 250.0 to 600.0 with a mean of 355.17 ± 257.15, C3 level ranged from 95.0 to 160.0 with a mean of 147.70 ± 17.44, and C4 level ranged from 84 to 160 with a mean of 64.57 ± 48.03. : shows the distribution of the studied SLE cases according to SLE-DAI Scores; 4 cases (11.4%) were with mild SLE-DAI, 5 cases (14.3%) were with moderate SLE-DAI, and 26 cases (74.3%) were with severe SLE-DAI. : shows comparison between the two studied groups according to sRAGE; in SLE sRAGE ranged from 1124.0 to 1413.0 with a mean of 1253.54 ± 66.74, while in the control group ranged from 824.0 to 1118.0 with a mean of 975 ± 25.01. : shows correlation between sRAGE and frequency of organ involvement among the studied group; there was a positive correlation between sRAGE and the presence of arthritis (r = 0.608∗, P < 0.001), fever and malaise (r = 0.602∗, P < 0.001), pericarditis (r = 0.624∗, P < 0.001), hair fall (r = 0.904∗, P < 0.001), and seizures (r = 0.624∗, P < 0.001). : shows correlation between sRAGE and C3, C4, ANA Anti-ds DNA, SLE-DAI, 24-h urine proteins, and protein/creatinine ratio; there was a positive correlation between sRAGE and SLE-DAI (r = 0.947∗, P < 0.001), 24-h urine proteins (r = 0.545∗, P < 0.001), and protein/creatinine ratio (r = 0.575∗, P < 0.001).
Table 1 Comparison between the two studied groups according to demographic data.
Table 2 The clinical description and frequency of organ affection among the studied group.
Table 3 Comparison between the two studied groups according to laboratory markers.
Table 4 Comparison between the two studied groups according to immunological markers. (ANA, Anti-ds DNA, C3, C4).
Table 5 Distribution of the studied SLE cases according to SLE-DAI scores.
Table 6 Comparison between the two studied groups according to sRAGE.
Table 7 Correlation between sRAGE and frequency of organ involvement among the studied group.
Table 8 Correlation between sRAGE and C3, C4, ANA Anti-ds DNA, SLE-DAI, 24-h urine proteins, and protein/creatinine ratio.
5 Discussion
Systemic lupus erythematosus (SLE) is a chronic, multifaceted inflammatory disease that can affect every organ system of the body. SLE is variable in its manifestations and follows a relapsing and remitting course.Citation36 HMGB-1 seems to be an important molecule in the pathophysiology of SLE by supporting the inflammatory clearance of apoptotic cells by binding to molecules released from apoptotic cells such as nucleosomes and DNA thereby increasing the immunogenicity of macrophages through receptors for advanced glycation end products (RAGE) to initiate cell signaling, this uptake accompanied by inflammation.Citation37,Citation38
The advanced glycation end products may bind to cell surface receptors to initiate cell signaling. The advanced glycation end products receptor includes R1, wR2, R3, scavenger receptors SCR-2, CD-36, and RAGE.Citation39
As plasma sRAGE levels increased in patients with SLE, this suggests that sRAGE may play a different role in the initial and progressive stages of the disease. However, the use of anti-lupus therapy could be involved in the triggering of a compensating mechanism that alters sRAGE production and/or regulation.
In our study, the plasma level of sRAGE in SLE studied group of patients, ranged from 1124.0 to 1412.0 with a mean of 1253 ± 66.74 while, in the control group sRAGE ranged from 824.0 to 1118.0 with a mean of 975.30 ± 25.01. There was a statistically significant difference between SLE patients and controls as regards sRAGE (P < 0.001). Also, there was a significant positive correlation between sRAGE and SLE-DAI (P < 0.001).
In a study carried by Martens et al.,Citation40 receptor for advanced glycation end products (RAGE) was associated with systemic lupus erythematosus disease activity and severity.
Another study carried by Ma et al.,Citation41 the role of high mobility group box-1 (HMGB1) protein and its receptors in autoimmune diseases and documented that HMGB1 is associated with disease activity in patients with SLE. Also, plasma sRAGE in patients receiving long-period treatment was significantly increased compared to those with short-period treatment and comparable with those in healthy control.
In another study done by Chavakis et al.,Citation42 sRAGE is a multiligand receptor that propagates cellular dysfunction in several inflammatory disorders. RAGE is expressed at low levels in normal tissues, but becomes upregulated at sites where its ligand accumulate. RAGE may play a dual role in the inflammatory responses. sRAGE interaction occurs on leukocytes or endothelial cells with its ligands results in cellular activation involving the transcription factor NF-kappa B: on the other hand, sRAGE on endothelial cells may function as an adhesive receptor that directly interacts with leukocyte SS2-integrins, thereby directly being involved in inflammatory cell recruitment.
In another study by Millard et al.Citation28 the formation of advanced glycation end products (AGEs) is a result of the non-enzymatic reaction between sugars and free amino groups of proteins. AGEs, through interaction with their specific receptor for AGEs (RAGE), result in activation of pro-inflammatory states and involved in numerous pathologic conditions. The soluble form of RAGE is able to act as a decoy to avoid interaction of RAGE with its pro-inflammatory ligands (AGES, HMGB1, S100 proteins).
The results of the study emphasize the possible involvement of the sRAGE pathway in the immunopathogenesis of SLE, which could lead it to have a potential therapeutic role.
6 Recommendations
HMGB1 is a nuclear DNA-binding protein that residues inside the nucleus detected in renal biopsy of patients with lupus nephritis. Further studies are promptly needed in order to evaluate the importance in determining urinary HMGB-1 and its value as a biomarker in lupus nephritis patients with renal involvement.
HMGB1 can be released to the extra-cellular space under specific conditions. Whereas HMGB1 is actively released from lipopolysaccharide (LPS), TNF, IL-1 activated monocytes and macrophages, its release also occurs passively during late phase of apoptosis as well as during necrosis.
Urinary biomarkers might be more relevant than serum biomarkers and could detect both systemic and local inflammation, they directly reflect renal pathology and possibly, a therapeutic target.
Conflict of interest
We have no conflict of interest to declare.
Notes
Peer review under responsibility of Alexandria University Faculty of Medicine.
Available online 23 July 2015
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