An in vitro assessment of growth promoting activity of a synthetic basic fibroblast growth factor (b-FGF) using Rama-27 cell line

Peer review under responsibility of Taibah University.

Abstract

Growth factors (GFs) are naturally occurring proteins or steroid hormones which act as signaling molecules between cells that play a key role in the processes of proliferation, cell differentiation and maturation of a wide variety of cells and tissues. A recently purified synthetic basic b-FGF was assessed using a routine tissue culture assay via application of a wide range of doses ranged between 0.1 and 300 ng/mL of the basic fibroblast growth factor (b-FGF) in phosphate buffer saline (PBS) and 10% fetal calf serum (FCS) on the growth rate of Rama-27 mammary cell line. Applying SPSS “Student T-test” biostatistics the result showed significant increase (p ≤ 0.05), almost 7 folds in tissue proliferation at a low dose of 0.3 ng/mL FGF in comparison with control tissue (PBS) only. It is concluded that 0.3 ng/mL dose represents the lower optimal dose suggesting its possibility of an in vivo technique to test its potency in curing skin wounds in rats.

Keywords:

• In vitro
• Rama-27
• Synthetic FGF
• Tissue culture

Acknowledgements

The authors do acknowledge Professor David Fernig for his generous gift of the synthesized b-FGF being used in this project. FOB does thank Drs. Thamir Ismael and Nicola Wells for their technical assistance regarding preparation of the doses.

Notes

Peer review under responsibility of Taibah University.