Abstract
The focus of the review is to show how the Box-Behnken Design (BBD) could be applied in poultry nutrition research. BBD is a multivariate experimental design which uses less treatment combinations and allows researchers to estimate and optimise processes. Box-Behnken design was used in an experiment to estimate growing broiler requirements for total sulphur amino acids (TSAA), lysine (Lys) and threonine (Thr). In the experiment BBD has shown to be a useful tool in estimating and optimizing nutrient requirements in growing broilers.