Abstract
Next generation sequencing (NGS) has supplanted traditional methods for microbiome analyses. Advantages include higher resolution and lower costs. Analyses have evolved from basic 16S rRNA surveys to metagenomic shotgun sequencing analyses. Limitations and obstacles still exist, e.g. 16S bias, bioinformatics bottlenecks, classification accuracies, and genome assembly using short reads. New tools are being developed to improve output and deal with larger amounts of data generated.
The work was supported by grants UH3CA140233, R01CA159036, R01AI063477 and U19DE018385 from the National Cancer Institute and the National Institute for Allergy and Infectious Diseases, and National Institute of Dental and Craniofacial Research.