Inhibition of experimental autoimmune uveoretinitis with anti-macrophage migration inhibitory factor antibodies

Nobuyoshi Kitaichi Department of Ophthalmology, School of Medicine, and Section of Pathology, Institute of Immunological Science, Central Research Institute, School of Medicine, Hokkaido University, Sapporo, Japan

Akira Matsuda Department of Ophthalmology, School of Medicine, and Section of Pathology, Institute of Immunological Science, Central Research Institute, School of Medicine, Hokkaido University, Sapporo, Japan

Satoshi Kotake Department of Ophthalmology, School of Medicine, and Section of Pathology, Institute of Immunological Science, Central Research Institute, School of Medicine, Hokkaido University, Sapporo, Japan

Kenichi Namba Department of Ophthalmology, School of Medicine, and Section of Pathology, Institute of Immunological Science, Central Research Institute, School of Medicine, Hokkaido University, Sapporo, Japan

Yoshitsugu Tagawa Department of Ophthalmology, School of Medicine, and Section of Pathology, Institute of Immunological Science, Central Research Institute, School of Medicine, Hokkaido University, Sapporo, Japan

Yoichi Sasamoto Department of Ophthalmology, School of Medicine, and Section of Pathology, Institute of Immunological Science, Central Research Institute, School of Medicine, Hokkaido University, Sapporo, Japan

Kazumasa Ogasawara Department of Ophthalmology, School of Medicine, and Section of Pathology, Institute of Immunological Science, Central Research Institute, School of Medicine, Hokkaido University, Sapporo, Japan

Kazuya Iwabuchi Department of Ophthalmology, School of Medicine, and Section of Pathology, Institute of Immunological Science, Central Research Institute, School of Medicine, Hokkaido University, Sapporo, Japan

Kazunori Onoé Department of Ophthalmology, School of Medicine, and Section of Pathology, Institute of Immunological Science, Central Research Institute, School of Medicine, Hokkaido University, Sapporo, Japan

Hidehiko Matsuda Department of Ophthalmology, School of Medicine, and Section of Pathology, Institute of Immunological Science, Central Research Institute, School of Medicine, Hokkaido University, Sapporo, Japan

Jun Nishihira Department of Ophthalmology, School of Medicine, and Section of Pathology, Institute of Immunological Science, Central Research Institute, School of Medicine, Hokkaido University, Sapporo, Japan

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Abstract

Purpose. The role of macrophage migration inhibitory factor (MIF) in the regulation of ocular autoimmune disease was studied in experimental autoimmune uveoretinitis (EAU) in rats following immunization with a retinal antigen (Ag), interphotoreceptor retinoid-binding protein (IRBP). Methods. LEW rats were immunized with a single injection of IRBP derived peptide, R16(ADGSSWEGVGVVPDV). A neutralizing monoclonal antibody (mAb, IgM) to MIF was injected intraperitoneally every second day from day 0 to day 6 (group A), or from day 8 to day 14 (group B). Control rats were treated with unrelated mouse IgM or PBS. T cell proliferative responses were measured 12 days after immunization. The occurrence and severity of EAU were observed and compared among experimental and control groups. Results. T cell proliferative responses against R16 were inhibited in rats treated with anti-MIF mAb compared with the control rats. The development of EAU was delayed in the rats of group A in comparison with those of group B and the control group. The mean histological EAU score on day 18 in group A was 1.11 ± 0.11 and significantly lower than those of the group B (1.29 ± 0.19) and the control (1.67 ± 0.19). Conclusions. The present result suggests that MIF plays an important role in induction of EAU.

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Inhibition of experimental autoimmune uveoretinitis with anti-macrophage migration inhibitory factor antibodies

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Inhibition of experimental autoimmune uveoretinitis with anti-macrophage migration inhibitory factor antibodies

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