Hydroxyl Radical Scavenging and Protective Role of Tephrosia purpurea in Gentamicin-Induced Kidney Cell Damage

Abstract

Tephrosia purpurea, commonly known as Sarphonka, is widely used in the traditional Indian system of medicine as an anti-inflammatory agent and also used in various liver, spleen and kidney disorders. The protective role of this plant has been investigated in gentamicin-induced rat kidney cortical cell damage. The alcohol extract of T. purpurea showed a significant hydroxyl radical scavenging activity in vitro. Using a Trypan blue exclusion assay, it was found that the extract markedly increased the percentage viability of the isolated rat kidney cortical cells in gentamicin-induced cell damage. By the evaluation of LDH activity and acid phosphatase content, it was established that the cell damage was minimized in the case of cells treated with the extract of T. purpurea. The hydroxyl radical scavenging effect of the extract was enhanced with increases in the concentration of drug, suggesting the role of free radical scavengers in minimizing gentamicin-induced kidney cell damage.