Abstract
Objective: The purpose of this study was to examine the effects of a dentifrice containing 5% calcium sodium phosphosilicate (CSP) on the remineralization of the enamel using optical coherence tomography (OCT).
Materials and methods: Bovine incisors were sliced and shaped in a rectangular form. One group of five specimens was treated with undersaturated 0.1 M lactic acid buffer solution (pH 4.75) for 10 min and then placed in artificial saliva (pH 7.0) (De group). Other specimens were stored in solutions of toothpaste containing CSP for 10 min, followed by 10-min immersion in the lactic acid buffer solution twice a day before storage in artificial saliva (CSP group). An additional group was stored in only artificial saliva (control group). OCT imaging on the selected location of the enamel surface was performed. The peak intensity and width at 1/e2 were recorded in each of the six areas on the sample and averaged, and the sample size of each group was six. The integrated value in units (dB × μm) was calculated in the area of peak intensity. The data for each group was subjected to one-way repeated-measures ANOVA and Tukey HSD tests (α = 0.05).
Results: The changes in integrated values of each group were different. A slight but significant increase in the integrated value was observed in the control group, whereas a slight but significant decrease in the value was observed the De group. Integrated values increased in the CSP group.
Conclusions: Remineralization occurred upon immersion in the toothpaste containing CSP.
Acknowledgements
This work was supported, in part, by Grants-in-Aid for Scientific Research (C) 26462896, 16K11564, and 16K11565 from the Japan Society for the Promotion of Science (JSPS). This project was also supported, in part, by the Sato Fund, and by a grant from the Dental Research Centre of Nihon University School of Dentistry, Japan.
Disclosure statement
The authors certify that they have no proprietary, financial, or other personal interest of any nature or kind in any product, service, and/or company that is presented in this article.
Notes on contributors
Saki Matsuyosh, DDS, is a Graduate Student at the Department of Operative Dentistry, Nihon University School of Dentistry.
Ryosuke Murayama, DDS, PhD, is a Research Assistant at the Department of Operative Dentistry, Nihon University School of Dentistry.
Shunsuke Akiba, DDS, is a Graduate Student at the Department of Operative Dentistry, Nihon University School of Dentistry.
Chiaki Yabuki, DDS, is a Graduate Student at the Department of Operative Dentistry, Nihon University School of Dentistry.
Toshiki Takamizawa, DDS, PhD, is an Associate Professor at the Department of Operative Dentistry, Nihon University School of Dentistry.
Hiroyasu Kurokawa, DDS, PhD, is an Associate Professor at the Department of Operative Dentistry, Nihon University School of Dentistry.
Masashi Miyazaki, DDS, PhD, is a Professor and Chair at the Department of Operative Dentistry, Nihon University School of Dentistry.