Association of PON1, TNF-α and TGF-β gene polymorphisms with prognosis in oral and oropharyngeal squamous cell carcinoma

Abstract

Objective

The oral and oropharyngeal squamous cell carcinoma (OOSCC) accounts for 90–95% of tumours in the oral cavity. Single nucleotide polymorphism (SNP) in the coding region of PON1, tumour necrosis factor-alpha (TNF-α) and transforming growth factor-beta (TGF-β) have been associated with to development of different cancers. Our aim was to investigate the prognostic value of PON1 (rs854560 and rs662), TNF-α (rs1800629 and rs361525) and TGF-β (rs1800469) SNPs in OOSCC.

Materials and methods

We genotyped 163 OOSCC patients and 146 patients from group of control for PON1 (rs854560 and rs662), TNF-α (rs1800629 and rs361525) and TGF-β (rs1800469) SNPs by real-time polymerase chain reaction (PCR).

Results

TNF-α (rs1800629) GG genotype was significantly more frequent in intraoral lesions and clinical stages III and IV, while the polymorphic AA genotype in lip lesion and clinical stages I and II. Moreover, TGF-β (rs1800469) AG and AA genotypes were significantly more frequent in larger tumours (T3 e T4). TNF-α (rs1800629) AG genotype had poor survival and patients carrying the PON1 (rs662) TT genotype tended to poor survival.

Conclusions

Results suggest that the rs1800629 and rs1800469 could exert influence in the more aggressive behaviour of OOSCC and the genotypes AG of rs1800629, and TT of rs662 could be markers with prognostic value in OOSCC.

Keywords:

• Oral cancer
• SNP
• PON1
• TNF-α
• TGF-β

Acknowledgments

This work has in part been presented at the 45th Brazilian Society of Oral Medicine and Oral Pathology (Santos KMS, Santos EM, Santos ITS, et al. Association of PON-1, TNF-α, and TGF-β gene polymorphisms with prognosis in oral and oropharyngeal squamous cell carcinoma. Oral Surg Oral Med Oral Pathol Oral Radiol. 2020;130(3): e250-e251).

Disclosure statement

The authors declare that they have no conflict of interest.

All authors have viewed and agreed to the submission of this article.

Additional information

Funding

This study was supported by the Brazilian National Council for Scientific and Technological Development (CNPq, Portuguese: Conselho Nacional de Desenvolvimento Científico e Tecnológico), Higher Education Personnel Improvement Coordination (CAPES, Portuguese: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) and Support Foundation for Research and Technological Innovation of the State of Sergipe (FAPITEC, Portuguese: Fundação de Apoio à Pesquisa e a Inovação Tecnológica do Estado de Sergipe).