Connections between the Dorsal Raphe Nucleus and a Hindbrain Region Consisting of the Cochlear Nucleus and Neighboring Structures

Abstract

Previous studies have shown that neurons in the raphe nuclei respond to acoustic stimuli. The present study investigated connections between the dorsal raphe nucleus (DRN) and a hindbrain region consisting of the cochlear nucleus (CN) and neighboring structures. A mixture of one or more tracers (cholera toxin B, biotinylated dextran amine (BDA), and 3H-leucine) was injected into the cat DRN. Retrograde-labeling results are presented whereby a new structure, to be called the juxta-acoustico-floccular fascicle (JAFF), is identified. The JAFF is surrounded by the CN, flocculus, lateral cerebellar nucleus, lateral vestibular nucleus, and restiform body. The JAFF is closely associated with the infracerebellar nucleus (ICN). Labeled neurons projecting to the DRN were concentrated in the JAFF, embedded among axons. Less numerous labeled neurons were in the ICN and CN. Anterograde-labeling results are presented showing fibers labeled with BDA or with BDA and ³H-leucine in the CN, cochlear nerve and vestibular nerve, indicating that the DRN projects to these structures. The ascending and descending connections between the DRN and the above hindbrain region may mediate a reflex that may alter the sensitivity of the auditory system in response to biologically salient (e.g. threatening or attractive) stimuli.

• Auditory Cerebellum Multisensory Estibular