Nasopharyngeal Carriage of Drug-resistant Streptococcus pneumoniae in Children with Acute Otitis Media Evaluated by Polymerase Chain Reaction-based Genotyping of Penicillin-binding Proteins

Muneki Hotomi From the Department of Otolaryngology Head and Neck Surgery, Wakayama Medical University, Wakayama, Japan and Department of Pediatrics, New York State University at Buffalo, Buffalo, New York, USA

Noboru Yamanaka From the Department of Otolaryngology Head and Neck Surgery, Wakayama Medical University, Wakayama, Japan and Department of Pediatrics, New York State University at Buffalo, Buffalo, New York, USA

Howard Faden From the Department of Otolaryngology Head and Neck Surgery, Wakayama Medical University, Wakayama, Japan and Department of Pediatrics, New York State University at Buffalo, Buffalo, New York, USA

Jun Shimada From the Department of Otolaryngology Head and Neck Surgery, Wakayama Medical University, Wakayama, Japan and Department of Pediatrics, New York State University at Buffalo, Buffalo, New York, USA

Masaki Suzumoto From the Department of Otolaryngology Head and Neck Surgery, Wakayama Medical University, Wakayama, Japan and Department of Pediatrics, New York State University at Buffalo, Buffalo, New York, USA

Akihiro Sakai From the Department of Otolaryngology Head and Neck Surgery, Wakayama Medical University, Wakayama, Japan and Department of Pediatrics, New York State University at Buffalo, Buffalo, New York, USA

Tadahito Saito From the Department of Otolaryngology Head and Neck Surgery, Wakayama Medical University, Wakayama, Japan and Department of Pediatrics, New York State University at Buffalo, Buffalo, New York, USA

Kiyonori Kuki From the Department of Otolaryngology Head and Neck Surgery, Wakayama Medical University, Wakayama, Japan and Department of Pediatrics, New York State University at Buffalo, Buffalo, New York, USA

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Abstract

A polymerase chain reaction (PCR)-based genotyping of the penicillin-binding protein (PBP) genes pbp1a , pbp2x and pbp2b was used to characterize Streptococcus pneumoniae isolated from the nasopharynx of children with acute otitis media (AOM). Mutations were observed in pbp1a , pbp2x and pbp2b genes in 36.5% of the strains. Decreased susceptibility to &#103 -lactam antibiotics was closely associated with the frequency of mutations in the three PBP genes. Of penicillin-intermediately-resistant S. pneumoniae strains, 54.5% appeared to be genetically similar to penicillin-resistant S. pneumoniae strains. Of penicillin-susceptible S. pneumoniae strains, 33.3% had mutations in the pbp2x gene and showed relatively high MICs to cephalosporins. Strains with mutations in the three PBP genes were often isolated from children &#104 2 years old. Evaluation of mutations in PBP genes using PCR will prove useful for studying the epidemiology of antibiotic resistance.

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Nasopharyngeal Carriage of Drug-resistant Streptococcus pneumoniae in Children with Acute Otitis Media Evaluated by Polymerase Chain Reaction-based Genotyping of Penicillin-binding Proteins

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Nasopharyngeal Carriage of Drug-resistant Streptococcus pneumoniae in Children with Acute Otitis Media Evaluated by Polymerase Chain Reaction-based Genotyping of Penicillin-binding Proteins

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