Detection of Epstein-Barr Virus in Nasal T-cell Lymphoma

Tomohiro Makoshi From the Departments of 1Otorhinolaryngolog y and 2Pathology, School of Medicine, and the 3Department of Molecular Diagnostics,School of Allied Health Sciences, Kitasato University, Kanagawa, Japan

Hiroomi Takahashi From the Departments of 1Otorhinolaryngolog y and 2Pathology, School of Medicine, and the 3Department of Molecular Diagnostics,School of Allied Health Sciences, Kitasato University, Kanagawa, Japan

Toru Kameya From the Departments of 1Otorhinolaryngolog y and 2Pathology, School of Medicine, and the 3Department of Molecular Diagnostics,School of Allied Health Sciences, Kitasato University, Kanagawa, Japan

Kazuo Yao From the Departments of 1Otorhinolaryngolog y and 2Pathology, School of Medicine, and the 3Department of Molecular Diagnostics,School of Allied Health Sciences, Kitasato University, Kanagawa, Japan

Yuichi Sato From the Departments of 1Otorhinolaryngolog y and 2Pathology, School of Medicine, and the 3Department of Molecular Diagnostics,School of Allied Health Sciences, Kitasato University, Kanagawa, Japan

Meijin Nakayama From the Departments of 1Otorhinolaryngolog y and 2Pathology, School of Medicine, and the 3Department of Molecular Diagnostics,School of Allied Health Sciences, Kitasato University, Kanagawa, Japan

Benio Tsuchiya From the Departments of 1Otorhinolaryngolog y and 2Pathology, School of Medicine, and the 3Department of Molecular Diagnostics,School of Allied Health Sciences, Kitasato University, Kanagawa, Japan

Makito Okamoto From the Departments of 1Otorhinolaryngolog y and 2Pathology, School of Medicine, and the 3Department of Molecular Diagnostics,School of Allied Health Sciences, Kitasato University, Kanagawa, Japan

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Abstract

The close relationship between Epstein-Barr virus (EBV) and nasal T-cell lymphoma (NTL) has frequently been reported. However, the status of the infection, either lytic or latent, is obscure. This study involved 16 patients with NTL. Phenotypes of lymphoma cells were examined by immunohistochemical staining using CD3, CD4, CD8, CD20 and CD45RO monoclonal antibodies. EBV-encoded small nuclear RNA (EBER)-1 and EBV Not I tandem repeat region were detected by reverse transcription, using a rapid ( &#104 60 min) in situ hybridization technique. Tumor cells expressed at least one T-cell marker, such as CD3, CD4, CD8 and CD45RO. CD20 was not detected in any of the cases. EBER-1 was identified in all cases; no Not I tandem DNA repeat was demonstrated. All cases demonstrated a T-cell phenotype. These data suggest that NTL is associated with EBV infection in the latent phase.

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Detection of Epstein-Barr Virus in Nasal T-cell Lymphoma

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Detection of Epstein-Barr Virus in Nasal T-cell Lymphoma

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