Expression of C-type lectin receptor mRNA in chronic otitis media with cholesteatoma

Abstract

Conclusions: The levels of expression of various C-type lectin receptors (CLRs) messenger ribo nucleic acids (mRNAs) were significantly higher in cholesteatomas than in normal skin, suggesting that these CLRs may be involved in the pathogenesis of cholesteatoma.

Objectives: Altered expression of pattern recognition receptors may be associated with immune responses in patients with cholesteatoma. This study assessed the levels of expression of CLR mRNAs in normal skin and in cholesteatoma.

Methods: Cholesteatoma specimens were obtained from 38 patients with acquired cholesteatoma. The levels of expression of various CLR mRNAs were assessed quantitatively using real-time RT-PCR (Reverse transcription polymerase chain reaction) and correlated with age, sex, the presence of bacteria, hearing level, frequency of surgery, and degree of ossicle destruction.

Results: The levels of CD206 (cluster of differentiation 206), DEC-205 (Dendritic and epithelial cell-205), MGL (monoacylglycerol lipase), CLEC5A (C-type lectin domain family 5 member A), Dectin-2 (dendrite cell-associated C-type lectin-2), BDCA2 (Blood dendritic cell antigen 2), Mincle, DCIR (dendritic cell immunoreceptor), Dectin-1, MICL (Myeloid inhibitory C type-like lectin), and CLEC12B (C-type lectin domain family 12, member B) mRNAs were significantly higher in cholesteatoma than in control skin samples (p < 0.05). The levels of CLEC5A (C-type lectin domain family 5 member) and Dectin-1 mRNAs were significantly higher in cholesteatomas with ≥2 than ≤1 destroyed ossicles (p < 0.05), and the levels of MGL, Mincle, Dectin-1, and CLEC12B mRNAs were significantly higher in recurrent than initial cholesteatoma specimens (p < 0.05). The level of CLEC5A mRNAs was significantly higher in patients with severe than mild-to-moderate hearing loss (p < 0.05).

Chinese abstract

结论 各种C型凝集素受体（CLR）媒介核糖核酸（mRNA）的表征水平在胆脂瘤中显著高于正常皮肤, 这表明这些CLR可能参与胆脂瘤的发病机制。

目的 模式识别受体的经改变的表征可能与胆脂瘤患者的免疫反应相关。这项研究评估了CLR mRNA在正常皮肤和胆脂瘤中的表征水平。

方法 胆脂瘤标本取自38例获得性胆脂瘤患者。使用实时RT-PCR（逆转录聚合酶链反应）定量评估各种CLR mRNA的表达水平, 并将其与年龄、性别、细菌的存在、听力水平、手术频率和小骨破坏程度相联系。

结果 CD206（分化群206）、DEC-205（树突状细胞和上皮细胞-205）、MGL（单酰基甘油脂肪酶）、CLEC5A（C型凝集素结构域族系5成员A）、Dectin-2（树突状细胞相关的C型凝集素-2）、BDCA2（血液树突状细胞抗原2）、Mincle、DCIR（树突状细胞免疫受体）、Dectin-1, MICL（髓样抑制性C型凝集素）和CLEC12B（族系12, 成员B）mRNA在胆脂瘤中显著高于对照组皮肤样品（p <0.05）。 CLEC5A（C型凝集素结构域族系5成员A）和Dectin-1 mRNAs的含量在胆脂瘤中为≥2, 显著高于损坏的小骨（p <0.05）, 其值为≤1。并且, 在复发 胆脂瘤中的 MGL、Mincle、Dectin-1和CLEC12B mRNAs的含量显着高于原发胆脂瘤标本（p <0.05）。严重听力损失的患者的CLEC5A mRNA值显着高于轻度至中度听力损失的患者（p <0.05）。

Keywords:

• C-type lectin receptor
• cholesteatoma
• otitis media
• bacteria

Disclosure statement

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.

Additional information

Funding

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (No. 2011-0030072).