Effects of glass ionomer cement on facial nerve: a clinical and histopathologic evaluation

Abstract

Objective: The aim of this study was to investigate the clinical and histopathologic effects of glass ionomer cement (GIC) on facial nerve.

Methods: Eight Wistar albino rats were included in the study. Under general anesthesia, bilateral facial nerves of rats were dissected. Saline was injected into right facial nerves, while GIC was dropped on left facial nerves. Facial nerve functions of the rats were evaluated using mustache and bling reflex scores everyday along 14 days. On the 14th day, rats were sacrificed, and facial nerves examined in terms of inflammation, granulation tissue, and foreign body reaction. The clinical and histopathologic changes on facial nerves were compared for both groups.

Results: In clinical evaluation, facial nerve functions did not significantly differ between two groups (p = .301). On histopathologic examination, bone cement group had significantly more foreign body reaction, granulation tissue, and inflammation compared with the control group (p = .001; p = .002; p = .003, respectively).

Conclusions: Bone cement directly applied on the nerve causes foreign body reaction increased inflammation and granulation tissue. Nonetheless it does not lead to a permanent facial nerve dysfunction.

Chinese abstract

结论: 直接将骨结合剂施于神经会引起异物反应, 增加炎症和肉芽组织。尽管如此, 它不会导致永久性面部神经功能障碍。

目的: 本研究之目的是调查玻璃离聚物粘固剂对面部神经的临床和组织病理的影响。

方法: 对8只Wistar白化大鼠进行研究。在全身麻醉下, 解剖大鼠的双侧面部神经。将盐水注射到右面神经中, 同时将玻璃离聚物粘固剂滴在左面部神经上。在十四天中, 每天根据胡须和闪光反射评分对大鼠的面部神经功能进行评价。在第14天, 结束大鼠生命, 然后检查面部神经的炎症、肉芽组织和异物反应。比较两组的面部神经的临床和组织病理的变化。

结果: 在临床评估中, 两组的面部神经功能没有显着差异 (p = 0.301) 。在组织病理检查中, 与对照组相比, 骨结合剂组明显地具有更多的异物反应、肉芽组织和炎症 (分别为p = 0.001; p = 0.002; p = 0.003) 。

Keywords:

• Facial nerve
• glass ionomer cement
• rat
• toxicity

Disclosure statement

The authors report no conflict of interest. The authors alone are responsible for the content and writing the paper.