Sex-related hormone receptor in laryngeal squamous cell carcinoma: correlation with androgen estrogen-ɑ and prolactin receptor expression and influence of prognosis

Abstract

Background: The expression and function of androgen receptors (AR) and estrogen receptor alpha (ER-ɑ) in laryngeal squamous cell carcinoma (LSCC) have remained controversial for decades. Prolactin receptor (PRLR) is a sex-related hormone, that has been rarely documented in terms of expression or function compared with data on other hormone receptors in laryngeal carcinoma.

Methods: This study reports on immunohistochemical and reverse transcription-polymerase chain reaction (RT-PCR) analysis of tumour tissue and adjacent normal tissue in 96 patients with LSCC (82 males and 14 females). The expression levels of the receptors were related to clinicopathologic parameters and survival data.

Results: At both protein and mRNA levels, the expression level of AR, ER-ɑ, and PRLR was much higher in LSCC than in adjacent normal tissues (p < .05). Among them, higher PRLR expression in tumour tissues tended to have a significantly poorer survival rate (p = .03) for patients with LSCC. Furthermore, higher expression of ER-ɑ in tumours was correlated with higher expression level of PRLR (r = .823, p = .03).

Conclusion: The findings of this study indicate that the sex-related hormone receptors play an important role in the development of LSCC. The PRLR represents a novel prognosticator, because of its negative effect on survival and its interaction with ER-ɑ.

Chinese abstract

背景：喉鳞状细胞癌（LSCC）中雄激素受体（AR）和雌激素受体α（ER-ɑ）的表达和功能几十年来一直存在争议。催乳素受体（PRLR）是一种性别相关的激素, 与喉癌中其他激素受体的资料相比, 在表达或功能方面有很少的记录。

方法：本研究报告了96例LSCC患者肿瘤组织和相邻正常组织的免疫组织化学和逆转录聚合酶链反应（RT-PCR）分析（82例男性和14例女性）。受体的表达水平与临床病理参数和存活资料相关。

结果：在蛋白质和mRNA水平上, LSCC中AR, ER-ɑ和PRLR的表达水平高于相邻正常组织（p < 0.05）。其中, 肿瘤组织中较高的PRLR表达往往表示LSCC患者显然较差的存活率（p = 0.03）。此外, ER-ɑ在肿瘤中的较高表达与PRLR的较高表达水平相关（r = .823, p = 0.03）。

结论：本研究结果表明, 性相关激素受体在LSCC发展中起重要作用。 PRLR代表了一种新型预后因子, 因为它对存活率及其与ER-ɑ的相互作用有负面影响。国家的能力。近几十年来, 中国研究人员在医学科学领域取得了长足的进步。然而, 耳鼻咽喉科出版物的数量和质量状况尚未有报道。本研究的目的是比较中国作者从中国大陆（MC）, 台湾（TW）和香港（HK）三个地区的产量。 方法：根据”科学引文索引扩大”（2015年）”科学核心收集”科目引用指数的主题类别”耳鼻咽喉科学”, 从43个耳鼻咽喉科杂志地

Keywords:

• Laryngeal carcinoma
• androgen receptor
• estrogen receptor alpha
• prolactin receptor
• overall survival

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was funded by National Natural Science Foundation of China (No 81600790) and special fund for the selection of outstanding young teachers in Colleges and universities in Shanghai (No 101005.001.14.115).