Abstract
Conclusions: We did not identify any strong associations between HPV-16 viral load and any of the clinical or lifestyle factors.
Objective: The epidemiology of oropharyngeal SCC is changing, with an increasing proportion of HPV-positive cases seen in the last decade. It is known that a high viral load is linked to the development of cervical cancer, the relation between viral load and oropharyngeal SCC is less clear. We sought to determine HPV-16 viral load in HPV-positive oropharyngeal SCCs using highly sensitive digital PCR and to identify clinical and lifestyle factors associated with viral load.
Subjects and methods: We analysed 45 HPV-16 positive oropharyngeal SCCs diagnosed between 2013 and 2015. All patients completed a lifestyle questionnaire and clinical data were extracted from medical charts. Viral load was determined using digital PCR assays for HPV-L1 and RNAseP.
Results: We found large variations in HPV-16 viral load from 1 to 930 copies per cell (median 34 copies per cell).
Chinese abstract
结论:我们没有发现HPV-16病毒载量与任何临床或生活方式因素之间的紧密关系。
目的:口咽鳞状细胞癌的流行病学正在发生变化, 过去十年中, HPV阳性病例的比例越来越高。众所周知, 高病毒载量与宫颈癌的发展有关, 而病毒载量与口咽SCC之间的关系还不甚明了。我们试图使用高敏感数字PCR来确定HPV阳性口咽SCC中的HPV-16病毒载量, 并识别出与病毒载量相关的临床和生活方式因素。
研究对象和方法:我们分析了2013年至2015年间诊断的45例HPV-16阳性口咽鳞状细胞癌。所有患者均完成了生活方式问卷调查;从医疗图表中提取临床数据。使用HPV-L1和RNAseP的数字PCR测定来确定病毒载量。
结果:我们发现HPV-16病毒载量差异巨大, 单个细胞中有从1至930个拷贝(中值为每细胞34个拷贝)。
Disclosure statement
No potential conflict of interest was reported by the authors.