https://orcid.org/0000-0002-1006-1670
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Abstract
Background
Human papillomavirus (HPV) plays a major etiological role in the increasing number of patients with head and neck squamous cell carcinoma (HNSCC).
Objectives
This study aimed at exploring the relationship between HPV infection and prognosis in patients with hypopharyngeal carcinoma (HPSCC).
Methods
We retrospectively analyzed 108 consecutive patients diagnosed with HPSCC from 2015 to 2018. Real-time fluorescent quantitative PCR and P16 immunohistochemistry were used to detect HPV infection in tissues of patients with hypopharyngeal carcinoma. The numbers of CD8, CD4 and Foxp3 cells in tumor parenchyma were obtained by immunohistochemical counting. Finally, the analysis was performed according to the clinicopathological variables and prognosis of the patients.
Results
Among 108 patients with HPSCC, 18 cases were detected by qPCR, and 16 subtypes accounted for the majority (77.8%). Kaplan–Meier analysis showed that HPV16+, higher CD8+, higher CD4+ and higher FoxP3+ TIL infiltration was strongly associated with superior three-year disease-free survival (DFS), cancer-specific survival (CSS) and overall survival (OS). Univariate analysis showed that HPV and CD4+ TIL had higher predictive value for prognosis.
Conclusions
HPV16 infection is significantly related to tumor immune infiltrating cells (TILs).
Chinese Abstract
背景：人乳头瘤病毒(HPV) 对于头颈部鳞状细胞癌 (HNSCC) 患者数量上升方面起着主要的病因学作用。
目的：本研究旨在探讨HPV感染与下咽癌 (HPSCC) 患者预后的关系。
方法：我们回顾性分析了 2015至2018 年108 名诊断为 HPSCC 的连续患者。实时荧光
定量PCR和P16免疫组化用于检测下咽癌患者组织的HPV感染。肿瘤实质中的CD8、CD4 和Foxp3 的细胞数量通过免疫组织化学计数获得。最后, 根据患者的临床病理变量和预后进行分析。
结果：108例HPSCC患者中, qPCR检测出18例, 大多数人 (77.8%) 检测出16个亚型。 Kaplan-Meier 分析显示, HPV16+、较高的CD8+、较高的CD4+ 和较高的 FoxP3+ TIL 浸润与较高的三年无病生存率 (DFS)、癌症特异性生存期 (CSS) 和总生存期 (OS) 密切相关。 单因素分析表明, HPV 和CD4+ TIL对预后有较高的预测价值。
结论：HPV16感染与肿瘤免疫浸润细胞（TILs）显著相关。
Disclosure statement
No potential conflict of interest was reported by the author(s).