![Sample our Environment & Agriculture journals, sign in here to start your access, latest two full volumes FREE to you for 14 days]({"type":"image" , "src" : "/sda/5934/TOC-Subject-Sample-2021-Environment-Agriculture.jpg"})
Abstract
Two allergenic substances (C 5 and P 4), which consisted of ribonucleic acid as their main component, were isolated from the heated extract of liver fluke (Fasciola hepatica) by means of precipitation by ammonium sulphate and phenol extraction, followed by extraction with potassium acetate and ethanol fractionation.
One of these substances, C 5, which was extracted with phenol was electrophoretically homogeneous, but ultracentrifugally was shown to contain one other substance in a small quantity.
The other substance, P 4, which was isolated from the phenol insoluble fraction was electrophoretically and ultracentrifugally homogeneous.
Both of these were almost the same in biological activity, and their solutions, which were diluted 1 : 100,000 with Ringer’s solution, were still active in the intradermal reaction to the fascioliasis of cattle. Judging from their activity and yield, it seems that all the allergenic substances which are in the heated extract of liver fluke, are represented by the protein allergen reported already, as well as by C 5 and P 4 described here.
Abstract
This paper deals with the composition of an allergenic substance which was isolated from liver fluke by means of phenol extraction and other methods. The preparation is composed of ribonucleic acid (95%) as its main component and a small amount of peptide (4.6%). The ribonucleic acid consists of nearly equal parts of adenylic, guanylic, uridylic and cytidylic acids, and the peptide is made up of aspartic acid, glutamic acid, glycine, cystine, alanine, serine, threonine, valine, leucine, histidine, lysine, and arginine.