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Abstract
It has been found that Gluconobacter liquefaciens metabolized 5-ketogluconic acid. In order to clarify metabolic pathways of this compound, the oxidation products by resting cells of this organism were investigated. Rubiginol, rubiginic, comenic, 2,5-diketogluconic, glycolic and tartronic acids were detected or identified in the reaction fluid. On the basis of these results and the data obtained by means of manometric experiments, the oxidation pathways of 5–ketogluconic acid were discussed.
Oxidation pathways of 5-ketogluconie acid by resting cells of Gluconobacter liquefaciens were further investigated. Arsenite inhibited the oxidation of this compound. The amount of carbonyl compounds in the oxidation products of 5–ketogluconic acid was increased by addition of 10-3 m arsenite. Pyruvic and α-ketoglutaric acids were identified among these carbonyl compounds. Members of the tricarboxylic acid cycle were oxidized actively by resting cells or cell-free extracts of this organism. These results suggested the presence of the tricarboxylic acid cycle in the terminal oxidatjon of 5-ketogluconic acid by this organism.