Abstract
Naringinase, which was induced from Aspergillus niger, consisted β-D-glucosidase and α-L-rhamnosidase. The former was successfully inactivated by heating the crude enzyme solution at 60°C and pH 6.4~6.8, whereas the latter was very stable under such treatment. By using this enzyme solution flavonoid gycosides were partially hydrolyzed and prunin from naringin, isosakuranin from poncirin, hesperetin-7-β-D-glucoside from hesperidin and neohesperidin, isoquercitrin from rutin, cosmociin from rhoifolin were obtained respectively in good yields. Furthermore kaempherol-3-robinobioside, a new flavonol glycoside, and afzelin were obtained from robinin and kaempheritrin, respectively.