Abstract
The chemical analyses showed that inositol deficiency caused especially the increase in content of glucan fraction and the decrease in contents of inositol, phospholipids and free-pool fraction. Other components, however, did not change in contents in inositol deficiency. More mannan fraction and free-pool substances were found to be released from the cells in inositol deficiency than in sufficiency. The respiratory and fermentative activities were lost in inositol deficient cells of 24 hr culture, which was considered to be the consequence of unbalanced growth death. But the respiratory activity did not so much decrease in inositol deficient cells of 8 hr culture as the fermentative activity, especially the aerobic fermentative activity, did. The release of mannan fraction and the decrease in intracellular free-pool fraction were accompanied with the loss of viability.
These results suggest that inositol deficiency caused the abnormality of the cell structure and permeability, and that this abnormality may be the possible cause of loss of viability due to inositol deficiency.