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Abstract
Changes were investigated in trace alkaloid composition of MC-1 (bright) and Burley-21 (burley) leaf lamina during curing. Seventeen alkaloids were identified. The total level of trace alkaloids decreased by approximately 60% in both MC-1 (flue-cured) and Burley-21 (air-cured). Although the level of most alkaloids also decreased, 2,3´-bipyridyl and four nornicotine derivatives, i.e. N´-hexanoyl-, N´-octanoyl-, 1´-(6-hydroxyoctanoyl)- and 1´-(7-hydroxyoctanoyl)-nornicotine, increased during curing in both varieties. Differences in the trace alkaloid composition of cured leaf lamina among MC-1 (bright), Burley-21 (burley) and Basma (Orient) were also studied. In all varieties, N´-formylnornicotine, nicotyrine, anabasine and cotinine were present at high concentrations relative to the other trace alkaloids. The alkaloids which were present in a relatively high concentration in particular varieties, were as follows: MC-1; N´-formylanabasine, Burley-21; N´-hexanoyl- and N´-octanoyl-nornicotine, and Basma; cotinine and N´-formylanatabine.