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Abstract
A water-insoluble glucan was synthesized by the β-transglycosylase of Trichoderma longibrachiatum in a reasonable yield from 1% cellopentaose as the substrate after 48 hr incubation. The glucan could be completely solubilized by cellulase to give glucose, cellobiose and cellotriose, although the reaction time was about 9 times longer than that required for the complete solubilization of the higher cellodextrin (DP 14) synthesized by the β-transglucosylase of Sclerotinia libertiana. The glucan was separated into two main fractions according to the solubility in NaOH solution, the major soluble in 4 N-NaOH and the minor insoluble in it. The structure of the major fraction was investigated by methylation analysis, and it was clearly shown to be a linear β-l,4-glucan having an average degree of polymerization of 19. These results indicated that the β-transglycosylase of Trichoderma longibrachiatum had a strict specificity of forming β-1,4-glucosidic linkages and also had a capacity to elongate the linkage to a higher extent.