Abstract
About 2700 strains of various kinds of microorganisms isolated from soil were tested for phospholipase D activity. All of the 200 selected strains were actinomycetes which were divided into two groups on the basis of the isomeric form of diaminopimelic acid (A2pm) present in the cell wall. Among 18 strains containing meso-A2pm, two strains were selected on the basis of taxonomical characteristics and productivity of phospholipase D. These two strains were examined for microbiological characteristics and found to belong to the genera Actinomadura and Nocardiopsis.