![Sample our Food Science & Technology journals, sign in here to start your access, latest two full volumes FREE to you for 14 days]({"type":"image" , "src" : "/sda/5936/TOC-Subject-Sample-2021-Food-Science-Technology.jpg"})
Abstract
Modulation of a pyridoxal 5ʹ-phosphate-synthesizing enzyme, pyridoxine (pyridoxamine) phosphate oxidase (EC 1.4.3.5), was investigated in wheat suspension culture. Pyridoxine–HCl added to the culture medium enhanced the pyridoxine 5ʹ-phosphate (PNP) oxidase activity, but not pyridoxamine 5ʹ-phosphate (PMP) oxidase activity. Thiamine–HCl also showed a .similar effect. The PNP oxidase activity was most effectively enhanced by potassium nitrate from 40 to 80 mM, which was added to the culture medium as a nitrogen source. On the other hand, PMP oxidase activity was stimulated by ammonium-type nitrogen.